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Terashima, Motoki; Nagao, Seiya*; Iwatsuki, Teruki; Fujitake, Nobuhide*; Seida, Yoshimi*; Iijima, Kazuki; Yoshikawa, Hideki
Journal of Nuclear Science and Technology, 49(8), p.804 - 815, 2012/08
Times Cited Count：12 Percentile：66.54(Nuclear Science & Technology)Terashima, Motoki; Seida, Yoshimi; Iwatsuki, Teruki; Iijima, Kazuki; Yoshikawa, Hideki; Yui, Mikazu; Nagao, Seiya*
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Eu(III) binding abilities of dissolved humic substances (HSs), which were extracted from deep groundwater in the Horonobe Underground Research Center, Hokkaido, Japan, were investigated by fluorescence quenching technique. In the synthetic groundwater condition, the fluorescence quenching due to the binding with Eu(III) was not observed for the groundwater HSs, while that was observed for the Aldrich humic acid (HA). This result indicates that the binding ability of the groundwater HSs are different from that of Aldrich HA. Based on structural characteristics of the groundwater HSs, the differences in the binding ability are discussed.
Terashima, Motoki; Nagao, Seiya*; Iwatsuki, Teruki; Sasaki, Yoshito; Seida, Yoshimi; Yoshikawa, Hideki
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no abstracts in English
Amano, Yuki; Nanjo, Isao; Iwatsuki, Teruki; Sasaki, Yoshito; Asano, Takahiro; Terashima, Motoki; Nagaoka, Toru*; Nakamura, Takamichi*; Yoshikawa, Hideki; Aoki, Kazuhiro
no journal, ,
In-situ experiment of redox reaction was conducted to assess the redox buffur capacity of water-rock-microbes system in the subsurface environment at 140 m depth of Horonobe Underground Research Laboratory. Dissolved oxygen was injected into a borehole, and the physico-chemical parameters (i.e. pH, ORP, DO, EC, etc.) and geochemical composition, microbial cell number, and microbial community structure were monitored continuously during the groundwater circulation. DO concentration could not detected after 10 hours from the injection. The concentration of Fe(II) was decreased with time, then the injected oxygen could be consumed by Fe(II). The rate of oxygen consumption during the experiment was 9.64 mg/L/day. The Eh value become stable at -208 mV after 5 days, then it showed that the reducing condition would be recovered quickly after closed condition.
Terashima, Motoki; Okazaki, Mitsuhiro; Iijima, Kazuki; Yoshikawa, Hideki
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no abstracts in English
Terashima, Motoki; Tachi, Yukio; Saito, Takumi*; Iijima, Kazuki; Shimoda, Satoko*; Nakazawa, Toshiyuki*; Yoshikawa, Hideki
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Terashima, Motoki; Saito, Takumi*; Iijima, Kazuki; Yoshikawa, Hideki
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no abstracts in English
Amano, Yuki; Ise, Kotaro; Terashima, Motoki; Sasaki, Yoshito; Amamiya, Hiroki; Yoshikawa, Hideki
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Microorganisms are widely distributed in the subsurface environments. However, the distribution, role and rate of metabolisms, and the origin of the activity are not well known. In this study, we investigated deep groundwater samples of sedimentary rocks, which is contained supersuturated methane and carbon dioxide, using boreholes in the Horonobe Underground Research Laboratory (URL), in the northern part of Hokkaido, Japan. Abundant methanogens were detected in the groundwater, and 80% of them were related to Methanoregula boonei and Methanobacterium flexile that can utilize hydrogen and carbon dioxide as methanogenesis. Phylotypes clustered within the phylum Firmicutes, beta-Proteobacteria, delta-Proteobacteria and Candidatte division TM7 were dominant in the groundwater samples. Laboratory experiment using culture a technique showed that humic substances purified from Horonobe area could be degraded by microorganisms. Our results suggest that microbial spatial distribution in the subsurface environment was correlate closely with geochemical conditions, such as redox condition and carbon sources.
Sakamoto, Mizuki*; Oki, Kensuke*; Yoshikawa, Motoki*; Terakado, Akihiro*; Nohara, Ryo*; Nojiri, Kumpei*; Nakashima, Yosuke*; Fukumoto, Masakatsu; Ichimura, Kazuya*; Hosoda, Yasunari*; et al.
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no abstracts in English
Nakashima, Yosuke*; Ichimura, Kazuya*; Takeda, Hisahito*; Iwamoto, Miki*; Hosoda, Yasunari*; Shimizu, Keita*; Oki, Kensuke*; Sakamoto, Mizuki*; Ono, Noriyasu*; Kado, Shinichiro*; et al.
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no abstracts in English
