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Morooka, Satoshi; Xu, P. G.; Shobu, Takahisa
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no abstracts in English
Oikawa, Kenichi; Nishiura, Shinsuke*; Watanabe, Kenichi*; Kiyanagi, Yoshiaki*; Sato, Hirotaka*; Ito, Masakazu*; Parker, J. D.*; Shinohara, Takenao
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no abstracts in English
Hasemi, Hiroyuki; Nakatani, Takeshi*; Inamura, Yasuhiro; Moriyama, Kentaro*
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no abstracts in English
Kurihara, Kazuo*; Hirano, Yu*; Hiromoto, Takeshi*; Kono, Fumiaki*; Tamura, Itaru; Tamada, Taro*
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no abstracts in English
Nakagawa, Hiroshi; Saio, Tomohide*; Nagao, Michihiro*; Inoue, Rintaro*; Sugiyama, Masaaki*; Tominaga, Taiki*; Kawakita, Yukinobu
no journal, ,
The flexible conformation of a multidomain protein is responsible for its biological function. 3-domain protein: MurD (47kDa) changes its domain conformation sequentially from open to semi-closed to closed conformation during enzymatic reactions. However, the dynamics of the domains in each conformation is unknown. In this study, we combined small-angle X-ray and neutron scattering (SAXS and SANS), dynamic light scattering (DLS), neutron back scattering (NBS), neutron spin echo (NSE), and molecular dynamics (MD) simulations to investigate the conformational dynamics of MurD in the three corresponding states (apo and ATP, inhibitor-bound state). The analysis showed that the conformational dynamics of MurD during the enzymatic reaction was not affected. The analysis suggests that the changes in domain dynamics during enzymatic reactions are related to the affinity and reaction efficiency with ligands that bind specifically to each reaction state.
Xu, P. G.; Morooka, Satoshi; Gong, W.; Suzuki, Tetsuya*; Harjo, S.; Shobu, Takahisa
no journal, ,
Shobu, Takahisa; Mukai, Tomohisa*; Ariki, Katsuyoshi*; Choe, H.*; Yamamoto, Shin*; Han, B.*; Morooka, Satoshi; Kurita, Keisuke; Iikura, Hiroshi
no journal, ,
In this study, we conducted basic research to evaluate the long-term adhesion characteristics of post-installed anchors by using the neutron beam from JRR-3 in the future. The experiments were carried out TNRF and RESA in JRR-3. In the cross-sectional CT image near the center of the concrete, multiple holes artificially created in the concrete inside the iron can with an inner diameter of about 60 mm were clearly photographed. As a result of comparing the dimensions with the actual measurement and the image, it was confirmed that the values were almost the same, and the tip of the hole could be observed with high accuracy.
Yamauchi, Hiroki; Kaneko, Koji; Osakabe, Toyotaka; Kubota, Masato; Hagihara, Masato
no journal, ,
no abstracts in English
Kurita, Keisuke; Iikura, Hiroshi; Shobu, Takahisa
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no abstracts in English
Hagihara, Masato; Torii, Shuki*; Yamauchi, Sara*; Kamiyama, Takashi*
no journal, ,
no abstracts in English
Kawasaki, Takuro; Harjo, S.; Gong, W.
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no abstracts in English
Kawakita, Yukinobu; Matsuura, Masato*; Tominaga, Taiki*; Yamada, Takeshi*; Tamatsukuri, Hiromu; Nakagawa, Hiroshi
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no abstracts in English
Takeda, Masayasu
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no abstracts in English
Gong, W.; Harjo, S.; Mayama, Tsuyoshi*; Kawasaki, Takuro; Aizawa, Kazuya; Tsuji, Nobuhiro*
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Harjo, S.; Kawasaki, Takuro; Gong, W.; Mao, W.; Aizawa, Kazuya
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no abstracts in English
Harjo, S.; Aizawa, Kazuya; Gong, W.; Kawasaki, Takuro
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no abstracts in English
Watanabe, Masao; Oku, Takayuki; Kawamura, Seiko; Takata, Shinichi; Su, Y. H.; Takahashi, Ryuta*; Yamauchi, Yasuhiro*; Nakamura, Masatoshi*; Ishikado, Motoyuki*; Sakaguchi, Yoshifumi*; et al.
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At Material Life Science Laboratory (MLF) in J-PARC, each beamline (BL) has standard sample environment (SE) equipment, while our SE team is organized to maintain BL common sample environment equipment and support the users. We have expensive equipment that is not frequently used but indispensable, and that requires specialized knowledge and skills for operation as BL common SE equipment. The SE team has sub-teams as;(1) cryo & magnet, (2) high temperature, (3) stress (high pressure, tension and fatigue machine), (4) soft matter, (5) humidity, (6) special environment (pulsed magnet, light irradiation and computation). In this presentation, we will explain the current status of BL common sample environment equipment.
Kajimoto, Ryoichi; Inamura, Yasuhiro
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no abstracts in English
Kumada, Takayuki; Miura, Daisuke*; Akutsu, Kazuhiro*; Torikai, Naoya*; Niizeki, Tomotake*
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Scattering of neutrons against protons remarkably depends on relative direction of their spins. Spin contrast variation is a technique to determine structure of composite materials using the polarization-dependent neutron scattering against proton. Recently, we have developed the first SCV neutron reflectivity technique for structural analysis of multilayer films. Structure of multilayer films can be determined from the proton-polarization dependent polarized neutron scatterings.
Hiroi, Kosuke; Motokawa, Ryuhei; Oba, Yojiro; Kumada, Takayuki; Okudaira, Takuya*; Oku, Takayuki; Suzuki, Junichi*
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no abstracts in English