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Oral presentation

Current status of neutron diffraction experiments on biological macromolecules using steady-state reactor

Kurihara, Kazuo; Tamada, Taro; Ohara, Takashi; Kuroki, Ryota

no journal, , 

no abstracts in English

Oral presentation

Crystallization and a preliminary neutron diffraction experiment of an antifreeze protein from Notched-fin eelpout

Ohara, Takashi; Adachi, Motoyasu; Tamada, Taro; Kuroki, Ryota; Kondo, Hidemasa*; Nishimiya, Yoshiyuki*; Tsuda, Sakae*

no journal, , 

no abstracts in English

Oral presentation

Crystallization of NADH-cytochrome ${it b}$$$_{5}$$ reductase aimed to neutron crystallography

Shoyama, Yoshinari; Tamada, Taro; Kuroki, Ryota; Kimura, Shigenobu*; Takeda, Kazuki*; Hayashi, Takuro*; Miki, Kunio*

no journal, , 

NADH-cytochrome ${it b}$ $$_{5}$$ reductase (b5R) is a pyridine nucleotide-dependent flavin reductase which contains a FAD in a molecule. The b5R catalyzes the electron transfer from NADH to cytochrome b5. The b5R is related to fatty acid metabolism and reduction of cytochrome P450. In order to clarify detailed structure of b5R, we carried out purification of b5R from E coli cell according to the method previously reported. Since a large-size crystal is necessary for the neutron structure analysis. We addressed enlargement of crystal by macroseeding method and periodical addition of protein sample to the crystallization solution. We succeeded in a preparation of large-scale crystal with volume of 4 mm$$^{3}$$, and we did preliminary neutron diffraction study using this crystal. Then we got 2${AA}$ resolution neutron diffraction data at BIX4.

Oral presentation

Neutron protein crystallography; For significant structural information for biology

Tamada, Taro; Ohara, Takashi; Kurihara, Kazuo; Kuroki, Ryota

no journal, , 

no abstracts in English

Oral presentation

Sequence dependencies of DNA deformability and hydration in the minor groove

Yonetani, Yoshiteru; Fujii, Satoshi*; Sarai, Akinori*; Kono, Hidetoshi; Go, Nobuhiro*

no journal, , 

no abstracts in English

Oral presentation

Structure analysis of HIV-1 protease by neutron diffraction

Adachi, Motoyasu; Ohara, Takashi; Kurihara, Kazuo; Tamada, Taro; Honjo, Eijiro; Okazaki, Nobuo; Arai, Shigeki; Shoyama, Yoshinari; Matsumura, Hiroyoshi*; Sugiyama, Shigeru*; et al.

no journal, , 

no abstracts in English

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