Feasibility study of X-ray imaging with near carbon K-shell edges on

竹本 邦子*; 鳴海 一成; 佐藤 勝也; 大東 琢治*; 難波 秀利*; 木原 裕*

no journal, , 

In order to actually observe a cell in the living state using X-ray microscopy, an observation method needs to be optimized. We propose a new method for observing cells in "carbon window" region, with energy slightly lower than that of the K-absorption edges of carbon instead of "water window" region. Using antifreeze solution instead of water, it is possible to decrease the temperature of biological specimens to subzero temperature without freezing. The low temperature promises reduction of radiation damage of the biological specimens. Since organic matters are quite transparent in carbon window region, contrast enhancing labeling technique is applied. Using the method, the label material binding site is clearly visualized like fluorescence of optical microscopy observation. In addition, because has been known to withstand radiation levels up to 1,000 times than that would kill normal human cells, X-ray imaging that makes an observation object will be more closely to the living condition.