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石井 里美; 鈴井 伸郎; 伊藤 小百合; 石岡 典子; 河地 有木; 大竹 憲邦*; 大山 卓爾*; 藤巻 秀
no journal, ,
To know kinetics of N fixation and transport of fixed N, a stable isotope,
N has been widely used. However, it has experimental limitations to analyze a quick (rapid) response to environmental changes such as temperature or light conditions with this method because it is invasive. We have developed imaging technique by using nitrogen gas labeled with a radio isotope
N (half life: 9.97 min), and PETIS (positron-emitting tracer imaging system) successfully, and we analyzed kinetics of fixation and fixed N quantitatively and noninvasively. In this study, we developed a rapid method to produce and purify
N-labeled nitrogen gas. The gas was mixed with 10 ml of O
and 5 ml of N
and 35 ml of helium, and the gas mixture was used in the tracer experiment. The tracer gas was fed to the underground part of intact nodulated soybean plants and serial images of distribution of
N were acquired by PETIS. The rates of nitrogen fixation of the six test plants were estimated as 0.17
0.10
mol N
h
from the dynamic data of PETIS images. The decreasing rates of assimilated nitrogen were also estimated as 0.01
20.011
mol N
h
.
藤巻 秀; 石井 里美; 鈴井 伸郎; 伊藤 小百合; 河地 有木; 大竹 憲邦*; 大山 卓爾*; 石岡 典子
no journal, ,
ポジトロンイメージング及び窒素13標識窒素ガストレーサを用いて、生きたダイズの根粒における共生的窒素固定の画像化と定量的解析を行った。ポジトロンイメージングは総合的な技術であり、近年ではわれわれはカドミウム107トレーサの製造などの技術開発を行ってきた。ダイズにおける窒素固定の研究へのポジトロンイメージング技術の応用について、おもにトレーサ開発などの技術論的観点から総合的に考察する。