Formation of rod-like particles with periodic interior by hydroxypropylmethyl cellulose samples with narrow molar mass distributions in aqueous solutions

Yoshida, Misato*; Arai, Kengo*; Nakagawa, Daiki*; Horikawa, Yoshiki*; Iwase, Hiroki*; Kumada, Takayuki; Motokawa, Ryuhei; Shikata, Toshiyuki*

Biomacromolecules, 26(11), p.8332 - 8342, 2025/11

https://doi.org/10.1021/acs.biomac.5c01969

Calixarene-assisted protein refolding via liquid-liquid extraction

Shimojo, Kojiro; Oshima, Tatsuya*; Naganawa, Hirochika; Goto, Masahiro*

Biomacromolecules, 8(10), p.3061 - 3066, 2007/10

https://doi.org/10.1021/bm070418q

We have found that a calix[6]arene acetic acid derivative forms a supramolecular complex with urea-denatured cytochrome c at the oil-water interface, which enables quantitative transfer of the protein from an 8 M urea aqueous solution into an organic phase through a proton-exchange mechanism. Denatured cytochrome c is completely separated from the denaturant, and is isolated from other denatured cytochrome c molecules to suppress the generation of aggregates due to protein-protein interactions. The recovery of cytochrome c from the organic phase is successfully achieved under acidic conditions using an appropriate amount of 1-butanol. UV-visible, CD, and fluorescence spectroscopic characterizations demonstrate that cytochrome c transferred into a denaturant-free aqueous solution regains its native structure. The reduction kinetics of refolded cytochrome c using ascorbic acid indicates that the protein provides approximately 72% of native activity as an electron-transfer protein.

Crown ether-mediated extraction and functional conversion of cytochrome in ionic liquids

Shimojo, Kojiro; Nakashima, Kazunori*; Kamiya, Noriho*; Goto, Masahiro*

Biomacromolecules, 7(1), p.2 - 5, 2006/01

https://doi.org/10.1021/bm050847t

no abstracts in English