Hydrogen and hydration in proteins

Niimura, Nobuo; Chatake, Toshiyuki; Kurihara, Kazuo; Maeda, Mitsuru

Cell Biochemistry and Biophysics, 40(3), p.351 - 369, 2004/06

https://doi.org/10.1385/CBB:40:3:351

Neutron diffraction provides an experimental method of directly locating hydrogen atoms in proteins. High resolution neutron diffractometers dedicated to biological macromolecules (BIX-type diffractometer) have been constructed at the Japan Atomic Energy Research Institute (JAERI) and they have been used in the 1.5 AA -resolution crystal structure analyses of several proteins.