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論文

DNA gyrase of ${it Deinococcus radiodurans}$ is characterized as Type II bacterial topoisomerase and its activity is differentially regulated by PprA in vitro

Kota, S.*; Rajpurohit, Y. S.*; Charaka, V. K.*; 佐藤 勝也; 鳴海 一成*; Misra, H, S.*

Extremophiles, 20(2), p.195 - 205, 2016/03

 被引用回数:6 パーセンタイル:47.77(Biochemistry & Molecular Biology)

The multipartite genome of ${it Deinococcus radiodurans}$ forms toroidal structure. It encodes topoisomerase IB and both the subunits of DNA gyrase (DrGyr) while lacks other bacterial topoisomerases. Recently, PprA a pleiotropic protein involved in radiation resistance in ${it D. radiodurans}$ has been suggested for having roles in cell division and genome maintenance. In vivo interaction of PprA with topoisomerases has also been shown. DrGyr constituted from recombinant gyrase A and gyrase B subunits showed decatenation, relaxation and supercoiling activities. Wild type PprA stimulated DNA relaxation activity while inhibited supercoiling activity of DrGyr. Thus, we showed that DrGyr confers all three activities of bacterial type IIA family DNA topoisomerases, which are differentially regulated by PprA, highlighting the significant role of PprA in DrGyr activity regulation and genome maintenance in ${it D. radiodurans}$.

論文

${it Deinococcus radiodurans}$ YgjD and YeaZ are involved in the repair of DNA cross-links

小野寺 威文; 佐藤 勝也; 太田 敏博*; 鳴海 一成

Extremophiles, 17(1), p.171 - 179, 2013/01

 被引用回数:6 パーセンタイル:76.68(Biochemistry & Molecular Biology)

Orthologs of ${it Escherichia coli ygjD}$ and ${it yeaZ}$ genes are highly conserved in various organisms. The genome of the radioresistant bacterium ${it Deinococcus radiodurans}$ possesses single orthologs of ${it ygjD}$ (DR_0382) and ${it yeaZ}$ (DR_0756). Complete loss of either one or both genes did not result in any significant changes in cell growth efficiency, indicating that both genes are not essential for cell viability in ${it D. radiodurans}$, unlike the case with other species such as ${it E. coli}$, ${it Bacillus subtilis}$ and ${it Saccharomyces cerevisiae}$. Survival rates following DNA damage induced by hydrogen peroxide, $$N$$-methyl-$$N'$$-nitro-$$N$$-nitrosoguanidine, ultra violet radiation, $$gamma$$-rays, cisplatin and mitomycin C (MMC) were compared among the wild-type strain and ${it D. radiodurans ygjD}$/${it yeaZ}$ null mutants. Interestingly, the null mutants exhibited high sensitivity to MMC, which mainly causes interstrand DNA cross-links. The sensitivity of the null mutants to MMC was restored to that of the wild-type by transformation with plasmids expressing these genes. These results suggest that ${it D. radiodurans ygjD}$ and ${it yeaZ}$ genes are involved in DNA repair and play a role in the repair of DNA cross-links in ${it D. radiodurans}$.

論文

Identification of PprM; A Modulator of the PprI-dependent DNA damage response in ${it Deinococcus radiodurans}$

大庭 寛史; 佐藤 勝也; Sghaier, H.; 柳沢 忠*; 鳴海 一成

Extremophiles, 13(3), p.471 - 479, 2009/05

 被引用回数:15 パーセンタイル:62.22(Biochemistry & Molecular Biology)

デイノコッカス・ラジオデュランスは、PprIが中心的な役割を果たすDNA損傷応答機構を持っており、RecAやPprAの発現を誘導している。このDNA損傷応答機構の特質をさらに明らかにするために、PprI依存性情報伝達経路の新たな因子の同定を試みた結果、コールドショックタンパク質(Csp)と相同性を持つ新たな制御タンパク質を発見し、このタンパク質をPprMと命名した。${it pprM}$遺伝子破壊株は、$$gamma$$線に著しく感受性を示した。PprMタンパク質は、デイノコッカス・ジオサーマリスやサーマス・サーモフィルスのCSPとともに亜群を形成する独特な分岐群に属していた。また、PprMタンパク質の働きによって、PprAの発現は誘導されるが、RecAの発現は誘導されなかった。PprMタンパク質は、大腸菌のCspDの場合と同様に、生理的条件下で2量体を形成していた。${it pprA}$ ${it pprM}$二重遺伝子破壊株は、どちらか一方の遺伝子が欠損した菌株よりも高い放射線感受性を示したことから、PprMタンパク質はPprAタンパク質以外の放射線耐性に重要な未知タンパク質を制御していると考えられた。

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