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Journal Articles

Application of real time PCR for the quantitative detection of radiation-induced genomic DNA strand breaks

Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; et al.

Journal of Insect Biotechnology and Sericology, 77(1), p.17 - 24, 2008/02

https://doi.org/10.11416/jibs.77.1_17

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with $$^{137}$$Cs $$gamma$$-rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by $$gamma$$-ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.

Journal Articles

Process of ${it in vivo}$ cyst formation from the implanted larval integument in the sweet potato hornworm, ${it Agrius convolvuli}$; A Simple model for studying wound healing

Fukamoto, Kana; Shirai, Koji*; Sato, Shigeru*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.99 - 106, 2006/10

https://doi.org/10.11416/jibs.75.99

no abstracts in English

Journal Articles

Effects of heavy-ion irradiation on the differentiation of epidermal cells in the silkworm, ${it Bombyx mori}$

Fukamoto, Kana; Shimura, Sachiko*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.107 - 114, 2006/10

https://doi.org/10.11416/jibs.75.107

no abstracts in English

Journal Articles

Regeneration of hemopoietic organs in the silkworm, ${it bombyx mori}$, after locally targeted irradiation with heavy ion beams

Ling, E.*; Fukamoto, Kana*; Xu, S.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*

Journal of Insect Biotechnology and Sericology, 72(2), p.95 - 100, 2003/09

https://doi.org/10.11416/jibs.72.95

no abstracts in English

Journal Articles

Why does hemocyte density rise during the wandering stage of the silkworm, ${it bombyx mori}$?

Ling, E.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*

Journal of Insect Biotechnology and Sericology, 72(2), p.101 - 109, 2003/09

https://doi.org/10.11416/jibs.72.101

no abstracts in English

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