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Journal Articles

Target irradiation of individual cells using focusing heavy-ion microbeam of JAEA-Takasaki, 5; Irradiation of individual cells with scanned heavy-ion microbeam

Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 73, 2015/03

Using a collimating heavy-ion microbeam system, we have explored various effects of heavy-ion hit on biological materials. However, there are limitations of the collimating system in the size of the microbeam spot and in the irradiation speed that cannot be overcome in principle. Thus, we started the development of a focusing microbeam system for target-irradiating individual cells more precisely. In this year, we established the protocol for irradiating "actual" cell sample with scanned beam. In the experiment, the HeLa cells were inoculated on a CR-39 film, then place on the sample stage. The microscopic image of cells was analyzed, and the cells were irradiated with scanned neon microbeam. After irradiation, we found the correspondence of the distribution pattern of the ion hit positions and the $$gamma$$-H2AX foci on cell nuclei, indicating rapid and accurate irradiation of individual cells with the focusing heavy-ion microbeam.

Journal Articles

Mechanisms for the induction of radioadaptive response by radiation-induced bystander response

Matsumoto, Hideki*; Tomita, Masanori*; Otsuka, Kensuke*; Hatashita, Masanori*; Maeda, Munetoshi*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Ikeda, Hiroko; et al.

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 76, 2015/03

The objective of this project is to elucidate molecular mechanisms for the induction of radioadaptive response through radiation-induced bystander responses induced by irradiation with heavy ion microbeams in JAEA. We found that the adaptive response was induced by Ar (520 MeV $$^{40}$$Ar$$^{14+}$$) microbeam-irradiation of a limited number of cells, followed by the broad beam-irradiation and that the adaptive response was almost completely suppressed by the addition of carboxy-PTIO, as a nitric oxide (NO) scavenger. In addition, we found several genes induced specifically and preferentially when radioadaptive response could be induced. We confirmed that ${it iNOS}$ expression was specifically induced only when radioadaptive response could be induced. Our findings strongly suggested that radioadaptive response can be induced by NO-mediated bystander responses evoked by irradiation with heavy ion microbeams.

Journal Articles

Analysis of bystander response in 3D cultured tissue induced by heavy-ion microbeam irradiation

Tomita, Masanori*; Matsumoto, Hideki*; Otsuka, Kensuke*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 77, 2015/03

Radiation-induced bystander responses are defined as responses in cells that have not been directly targeted by radiation but are in the neighborhood of cells that have been directly exposed. In this study, we aim to clarify a role of bystander response to sustain the homeostasis of damaged tissue using heavy-ion microbeams. We established the heavy-ion microbeam irradiation method to a 3D cultured human epidermis. Using this method, a viable cell rate of the 3D cultured human epidermis irradiated with 260 MeV $$^{20}$$Ne-ion microbeams or broadbeams was analyzed by the MTT method.

Journal Articles

Ion-species dependent bystander mutagenic effect on ${it HPRT}$ locus in normal human fibroblasts induced by C-, Ne- and Ar-ion microbeams

Suzuki, Masao*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Suzuki, Michiyo; Ikeda, Hiroko; Hattori, Yuya; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 78, 2015/03

We have been studying the radiation-quality dependent bystander cellular effects, such as cell killing, mutation induction and chromosomal damage, using heavy-ion microbeams with different ion species. This year we focused on the ion-species dependent bystander mutagenic effect on ${it HPRT}$ locus in normal human fibroblasts. The confluent culture were irradiated using a 256 (16$$times$$16)-cross-stripe method using C, Ne and Ar microbeam. Gene mutation on ${it HPRT}$ locus was detected with 6-thioguanine resistant clones. The mutation frequency in cells irradiated with C-ion microbeams was 6 times higher than that of non-irradiated control cells and of the sample treated with specific inhibitor of gap-junction cell-to-cell communication. On the other hand, no enhanced mutation frequencies were observed in cells irradiated with either Ne- or Ar-ion microbeams. There is clear evidence that the bystander mutagenic effect via gap-junction communication depends on radiation quality.

Journal Articles

Medaka blastoderm cells are capable of compensating the injured cells irradiated by carbon-ion micro-beam

Yasuda, Takako*; Oda, Shoji*; Asaka, Tomomi*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Yasuhiko; Mitani, Hiroshi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 85, 2015/03

In this present study, we examined the effects of heavy carbon-ions on development in pre-implantation period utilizing medaka blastula stage embryos (st. 11: blastderm diameter is about 500 $$mu$$m). We performed targeted irradiation by carbon-ion micro-beam (diameters of 120, 180 $$mu$$m) to a central parts of blastoderm and observed the abnormalities during development compared with whole-body irradiated embryos. As a results, retardation and characteristic malformed eyes were observed during development when blastoderm cells were partially irradiated, However, more than half of 50 Gy-irradiated embryos (area size=120 $$mu$$m diameter) could hatch normally in contrast to all embryos with 2 Gy of whole-body irradiation being lethal before hutching.

Journal Articles

Genetic changes in progeny of bystander human fibroblasts after microbeam irradiation with X-rays, protons or carbon ions; The Relevance to cancer risk

Autsavapromporn, N.*; Plante, I.*; Liu, C.*; Konishi, Teruaki*; Usami, Noriko*; Funayama, Tomoo; Azzam, E.*; Murakami, Takeshi*; Suzuki, Masao*

International Journal of Radiation Biology, 91(1), p.62 - 70, 2015/01

 Times Cited Count:19 Percentile:6.73(Biology)

Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful effects in progeny of bystander cells. Confluent human skin fibroblasts were exposed to microbeam radiations with different linear energy transfer (LET) by which 0.036$$sim$$0.4% of the cells were directly targeted by radiation. Following 20 population doublings, the cells were harvested and assayed for micronucleus formation, gene mutation and protein oxidation. The results showed that expression of stressful effects in the progeny of bystander cells is dependent on LET.

Journal Articles

Observation of radiation damage induced by single-ion hits at the heavy ion microbeam system

Kamiya, Tomihiro; Sakai, Takuro; Hirao, Toshio; Oikawa, Masakazu*

Nuclear Instruments and Methods in Physics Research B, 181(1-4), p.280 - 285, 2001/07

 Times Cited Count:2 Percentile:78.21

no abstracts in English

Journal Articles

Fate mapping of the cellular blastoderm stage egg of the silkworm, ${{it Bombyx mori}}$, by localized irradiation of heavy ion microbeams

Kiguchi, Kenji*; Kinjo, Y.*; Masahashi, K.*; Tu, Z.; Tamura, Hajime*; Shirai, K.*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

JAERI-Review 2000-024, TIARA Annual Report 1999, p.51 - 53, 2000/10

no abstracts in English

Journal Articles

Radio-microsurgery using heavy ion microbeams

Kiguchi, Kenji*; Kobayashi, Yasuhiko

Hoshasen To Sangyo, (85), p.30 - 36, 2000/03

no abstracts in English

Journal Articles

Effects of heavy ion microbeams on embryogenesis in the silkworm, ${{it Bombyx mori}}$; Morphological changes of the nuclei and cells after spot irradiation

Kiguchi, Kenji*; Shima, T.*; Kinjo, Y.*; Tu, Z. L.*; Yamasaki, Shuhei*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

JAERI-Review 99-025, TIARA Annual Report 1998, p.53 - 55, 1999/10

no abstracts in English

Journal Articles

Development of a collimated heavy ion microbeam system for irradiating mammalian cells individually

Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi; Yamamoto, Kazuo

JAERI-Review 99-025, TIARA Annual Report 1998, p.50 - 52, 1999/10

no abstracts in English

Journal Articles

Effects of local irradiation of heavy ion beams to the hematopoietic organs of the silkworm, Bombyx mori

Tu, Z. L.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

Nippon Sanshigaku Zasshi, 68(6), p.491 - 500, 1999/00

no abstracts in English

Journal Articles

An Automated single ion hit at JAERI heavy ion microbeam to observe individual radiation damage

Kamiya, Tomihiro; Sakai, Takuro; *; *; Hirao, Toshio

Nuclear Instruments and Methods in Physics Research B, 158(1-4), p.255 - 259, 1999/00

 Times Cited Count:6 Percentile:52.28(Instruments & Instrumentation)

no abstracts in English

Journal Articles

Fine single ion hit pattern drawing with heavy ion microbeams

Kamiya, Tomihiro; Sakai, Takuro; *; Hirao, Toshio

Dai-11-Kai Tandemu Kasokuki Oyobi Sono Shuhen Gijutsu No Kenkyukai Hokokushu, p.126 - 128, 1998/07

no abstracts in English

Journal Articles

JAERI heavy ion microbeam single ion hit experiment

Kamiya, Tomihiro; Sakai, Takuro; *; *; Hirao, Toshio

F-113-'98/NIES, p.60 - 63, 1998/00

no abstracts in English

Journal Articles

Diagnosis of the profile of the heavy-ion microbeam and estimation of the aiming accuracy of the single-ion-hit with using CR-39

*; Hirao, Toshio; Nashiyama, Isamu; Sakai, Takuro; Kamiya, Tomihiro; *; Ishii, Keizo*

Radiation Physics and Chemistry, 53(5), p.461 - 467, 1998/00

 Times Cited Count:0 Percentile:100(Chemistry, Physical)

no abstracts in English

Journal Articles

Recent progress in JAERI single ion hit system

; *; Suda, Tamotsu*; Hirao, Toshio; Kamiya, Tomihiro

Nuclear Instruments and Methods in Physics Research B, 130(1-4), p.498 - 502, 1997/00

 Times Cited Count:15 Percentile:23.87(Instruments & Instrumentation)

no abstracts in English

Journal Articles

Sub-micron microbeam apparatus for high resolution materials analyses

Kamiya, Tomihiro; Suda, Tamotsu*; Tanaka, Ryuichi

Nuclear Instruments and Methods in Physics Research B, 118(1-4), p.447 - 450, 1996/09

 Times Cited Count:31 Percentile:8.2(Instruments & Instrumentation)

no abstracts in English

Journal Articles

Beam positioning and single ion hit system in the high energy heavy ion microbeam apparatus

Kamiya, Tomihiro; ; Suda, Tamotsu*; *

BEAMS 1995: Dai-6-Kai Ryushisen No Sentanteki Oyo Gijutsu Ni Kansuru Shimpojiumu, 0, p.127 - 130, 1995/00

no abstracts in English

Journal Articles

Ion microbeam technique and development for its new application

Tanaka, Ryuichi; Kamiya, Tomihiro

Hoshasen, 20(3), p.21 - 32, 1994/00

no abstracts in English

32 (Records 1-20 displayed on this page)