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Journal Articles

Ectopic Endoreduplication caused by sterol alteration results in serrated petals in ${it Arabidopsis}$

Hase, Yoshihiro; Fujioka, Shozo*; Yoshida, Shigeo*; Sun, G.; Umeda, Masaaki*; Tanaka, Atsushi

Journal of Experimental Botany, 56(414), p.1263 - 1268, 2005/04

 Times Cited Count:41 Percentile:64.65(Plant Sciences)

The ${it Arabidopsis frill1}$ (${it frl1}$) mutant, that has serrated petals and sepals but no other large changes in plant morphology, was studied. The ${it frl1}$ had a mutation in ${it STEROL METHYLTRANSFERASE 2}$ and an altered sterol composition. It was found that the ${it frl1}$ mutation causes ectopic endoreduplication in petal tips that do not normally endoreduplicate. The rosette leaves of ${it frl1}$ also showed an enhanced level of endoreduplication, but their morphology was hardly affected. These facts suggest that the suppression of endoreduplication is important for petal morphogenesis and the normal sterol composition is required for this suppression.

Journal Articles

${it TRANSPARENT TESTA 19}$ is involved in the accumulation of both anthocyanins and proanthocyanidins in ${it Arabidopsis}$

Kitamura, Satoshi; Shikazono, Naoya; Tanaka, Atsushi

Plant Journal, 37(1), p.104 - 114, 2004/01

 Times Cited Count:410 Percentile:99.11(Plant Sciences)

Flavonoid compounds such as anthocyanins and proanthocyanidins (PAs; so-called condensed tannins) must be transported from the site of synthesis in the cytosol to the vacuoles. Novel Arabidopsis mutants, transparent testa 19 (tt19), which were induced by ion beam irradiation, showed a great reduction of anthocyanin pigments in the vegetative parts as well as brown pigments in the seed coat. The TT19 gene was isolated by chromosome walking and the gene candidate approach, and was shown to be a member of the Arabidopsis glutathione S-transferase (GST) gene family. Heterologous expression of a putative ortholog, petunia AN9, in tt19 complemented the anthocyanin accumulation but not the brown pigmentation in the seed coat. This suggests that the TT19 gene is required for vacuolar uptake of anthocyanins into vacuoles, but that it has also a different function from AN9. The depositional pattern of PA precursors in the mutant was different from that in the wild type. These results indicate that TT19 participates in the PA pathway as well as the anthocyanin pathway of Arabidopsis.

Journal Articles

Disruption of the ${it AtREV3}$ gene causes hypersensitivity to Ultraviolet B light and $$gamma$$-rays in Arabidopsis; Implication of the presence of a translesion synthesis mechanism in plants

Sakamoto, Ayako; Lan, V. T. T.; Hase, Yoshihiro; Shikazono, Naoya; Matsunaga, Tsukasa*; Tanaka, Atsushi

Plant Cell, 15(9), p.2042 - 2057, 2003/09

 Times Cited Count:81 Percentile:83.37(Biochemistry & Molecular Biology)

In order to investigate UV-response mechanisms in higher plants, we isolated a UV-sensitive mutant, ${it rev3-1}$, in ${it Arabidopsis}$. The root growth of ${it rev3-1}$ was inhibited after UV-B irradiation under both photoreactivating and non-photoreactivating conditions. We found that chromosome 1 of ${it rev3-1}$ was broken at least at three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase $$zeta$$ (${it AtREV3}$), which is thought to be involved in translesion synthesis. The ${it rev3-1}$ seedlings were also sensitive to $$gamma$$-rays and MMC, which are known to inhibit DNA replication. The incorporation of BrdU after UV-B irradiation was less in ${it suv1}$ than in the wild type plant. These results suggest that UV-damaged DNA interrupted DNA replication in the ${it rev3-1}$ mutant, leading to the inhibition of cell division and root elongation.

Journal Articles

Mutation rate and novel ${it tt}$ mutants of ${it Arabidopsis thaliana}$ induced by carbon ions

Shikazono, Naoya; Yokota, Yukihiko*; Kitamura, Satoshi; Suzuki, Chihiro*; Watanabe, Hiroshi; Tano, Shigemitsu; Tanaka, Atsushi

Genetics, 163(4), p.1449 - 1455, 2003/04

Irradiation of ${it Arabidopsis thaliana}$ by carbon ions was carried out to investigate the mutational effect of ion particles in higher plants. Frequencies of embryonic lethals and chlorophyll deficient mutants were found to be significantly higher after carbon ion-irradiation than after electron-irradiation (11-fold and 7.8-fold per unit dose, respectively). During the isolation of mutants, two new classes of flavonoid mutants (${it tt18, tt19}$) were isolated from carbon ion-mutagenized M2 plants. From PCR and sequence analysis, two of the three ${it tt18}$ mutant alleles were found to have a small deletion within the ${it LDOX}$ gene and the other was revealed to contain a rearrangement. Using the segregation rates, the mutation rate of carbon ions was estimated to be 17-fold higher than that of electrons. The isolation of novel mutants and the high mutation rate suggest that ion particles can be used as a valuable mutagen for plant genetics.

Journal Articles

LET dependence of lethality in ${it Arabidopsis thaliana}$ irradiated by heavy ions

Shikazono, Naoya; Tanaka, Atsushi; Kitayama, Shigeru*; Watanabe, Hiroshi; Tano, Shigemitsu*

Radiation and Environmental Biophysics, 41(2), p.159 - 162, 2002/04

 Times Cited Count:41 Percentile:70.86(Biology)

no abstracts in English

Journal Articles

FRL1 is required for petal and sepal development in arabidopsis

Hase, Yoshihiro; Tanaka, Atsushi; Baba, Tomohiro*; Watanabe, Hiroshi

Plant Journal, 24(1), p.21 - 32, 2000/10

 Times Cited Count:47 Percentile:70.55(Plant Sciences)

no abstracts in English

JAEA Reports

None

; Tokizawa, Takayuki; ; Nagara, Shuichi*

JNC TY6400 2000-014, 39 Pages, 2000/05

JNC-TY6400-2000-014.pdf:1.35MB

None

Journal Articles

The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis

Choe, S.*; Dilkes, B. P.*; Gregory, B. D.*; Ross, A. S.*; Yuan, H.*; Noguchi, Takahiro*; Fujioka, Shozo*; Takatsuto, Suguru*; Tanaka, Atsushi; Yoshida, Shigeo*; et al.

Plant Physiology, 119(3), p.897 - 907, 1999/03

 Times Cited Count:205 Percentile:97.49(Plant Sciences)

no abstracts in English

Journal Articles

The Arabidopsis dwf7/ste1 mutant is defective in the $$Delta^{7}$$ sterol C-5 desaturation step leading to brassinosteroid biosynthesis

Choe, S.*; Noguchi, Takahiro*; Fujioka, Shozo*; Takatsuto, Suguru*; Tissier, C. P.*; Gregory, B. D.*; Ross, A. S.*; Tanaka, Atsushi; Yoshida, Shigeo*; Tax. F. E.*; et al.

Plant Cell, 11, p.207 - 221, 1999/02

 Times Cited Count:214 Percentile:97.70(Biochemistry & Molecular Biology)

no abstracts in English

Journal Articles

A New arabidopsis mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa

; Tano, Shigemitsu*; T.Chantes*; Yokota, Yukihiko*; Shikazono, Naoya; Watanabe, Hiroshi

Genes and Genetic Systems, 72(3), p.141 - 148, 1997/06

 Times Cited Count:52 Percentile:72.97(Biochemistry & Molecular Biology)

no abstracts in English

Journal Articles

A New ${it Arabidopsis}$ mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa

Tanaka, Atsushi; Tano, Shigemitsu*; Chantes, T.*; Yokota, Yukihiko*; Shikazono, Naoya; Watanabe, Hiroshi

Genes and Genetic Systems, 72(3), p.141 - 148, 1997/00

 Times Cited Count:52 Percentile:72.45(Biochemistry & Molecular Biology)

no abstracts in English

Journal Articles

Effects of heavy ions on the germination and survival of arabidopsis thaliana

; Shikazono, Naoya; Yokota, Yukihiko*; Watanabe, Hiroshi; Tano, Shigemitsu*

International Journal of Radiation Biology, 72(1), p.121 - 127, 1997/00

no abstracts in English

Journal Articles

UV-B resistant mutations in Arabidopsis thaliana induced by ion beam irradiation

; Tano, Shigemitsu*; Watanabe, Hiroshi; Shikazono, Naoya; Yokota, Yukihiko*

Ikushugaku Zasshi, 46(SUPPL.1), 60 Pages, 1996/00

no abstracts in English

Journal Articles

Biological effects of heavy charged particles in Arabidopsis thaliana

; Yokota, Yukihiko*; Watanabe, Hiroshi; Shikazono, Naoya; Tano, Shigemitsu*

GSI-95-10, 0, p.87 - 90, 1995/00

no abstracts in English

Journal Articles

Effects of ion beams on arabidopsis thaliana(Abstract)

; Yokota, Yukihiko*; Shikazono, Naoya; Watanabe, Hiroshi; Tano, Shigemitsu*

Radiation Research 1985-1995, Congress Proc., Vol. I, 0, 445 Pages, 1995/00

no abstracts in English

15 (Records 1-15 displayed on this page)
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