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Journal Articles

A New visualization technique for the study of the accumulation of photoassimilates in wheat grains using [$$^{11}$$C]CO$$_{2}$$

Matsuhashi, Shimpei; Fujimaki, Shu; Uchida, Hiroshi*; Ishioka, Noriko; Kume, Tamikazu

Applied Radiation and Isotopes, 64(4), p.435 - 440, 2006/04

 Times Cited Count:18 Percentile:77.84(Chemistry, Inorganic & Nuclear)

no abstracts in English

JAEA Reports

Proceedings of the 3rd Workshop on Ion-beam-applied Biology; September 29, 2004, JAERI, Takasaki

Matsuhashi, Shimpei; Suzui, Nobuo; Ishioka, Noriko

JAERI-Conf 2005-010, 79 Pages, 2005/09

JAERI-Conf-2005-010.pdf:8.08MB

no abstracts in English

Journal Articles

A Report on TIARA Research Review Meeting

Arakawa, Kazuo

Hoshasen Kagaku, (80), p.57 - 59, 2005/09

no abstracts in English

Journal Articles

Quantitative modeling of photoassimilate flow in an intact plant using the Positron Emitting Tracer Imaging System (PETIS)

Matsuhashi, Shimpei; Fujimaki, Shu; Kawachi, Naoki; Sakamoto, Koichi; Ishioka, Noriko; Kume, Tamikazu

Soil Science and Plant Nutrition, 51(3), p.417 - 423, 2005/06

 Times Cited Count:23 Percentile:53.04(Plant Sciences)

no abstracts in English

Journal Articles

Development of positron-emitting bromine nuclides production using an isotope separator

Fateh, B.; Ishioka, Noriko; Watanabe, Satoshi; Kume, Tamikazu; Sekine, Toshiaki*

JAERI-Review 2003-033, TIARA Annual Report 2002, p.259 - 261, 2003/11

no abstracts in English

Journal Articles

Research of new-functions in a plant using the positron imaging method

Matsuhashi, Shimpei

Isotope News, (592), p.2 - 7, 2003/08

no abstracts in English

Journal Articles

Transfer function analysis of positron-emitting tracer imaging system (PETIS) data

Keutgen, N.; Matsuhashi, Shimpei; Mizuniwa, Chizuko; Ito, Takehito*; Fujimura, Takashi; Ishioka, Noriko; Watanabe, Satoshi; Sekine, Toshiaki; Uchida, Hiroshi*; Hashimoto, Shoji

Applied Radiation and Isotopes, 57(2), p.225 - 233, 2002/09

 Times Cited Count:9 Percentile:52.78(Chemistry, Inorganic & Nuclear)

no abstracts in English

Journal Articles

Real-time [$$^{11}$$C]methionine translocation in barley in relation to mugineic acid phytosiderophore biosynthesis

Bughio, N.*; Nakanishi, Hiromi*; Kiyomiya, Shoichiro*; Matsuhashi, Shimpei; Ishioka, Noriko; Watanabe, Satoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; Osa, Akihiko; Kume, Tamikazu; et al.

Planta, 213(5), p.708 - 715, 2001/09

 Times Cited Count:16 Percentile:38.22(Plant Sciences)

[$$^{11}$$C]Methionine ([$$^{11}$$C]Met) was supplied through barley roots and the $$^{11}$$C signal was follwoed using a real-time imaging system (PETIS), with subsequent development of autoradiographic images of the whole plant. In all cases, [$$^{11}$$C]Met was first translocated to the discrimination center, and this part was strongly labeled. Met absorbed by roots of the plants was subsequently translocated to other parts of the plant. In Fe-deficient (-Fe) barley plants, a drastic reduction in [$$^{11}$$C]Met translocation from the roots to the shoot was observed, while a greater amount of $$^{11}$$C was found in the leaves of Fe-sufficient or Met-pretreated -Fe plants. Treatment of -Fe plants with amiooxyacetic acid increased the translocation of [$$^{11}$$C]Met to the shoot. The retention of exogenously supplied [$$^{11}$$C]Met in the roots of -Fe barley indicates that the Met is used in the biosynthesis of mugineic acid (MA) in barley roots. This and the absence of Met movement from shoots to the roots suggest that the MA phytosiderophores precursor Met originates in the roots of plants.

Journal Articles

Vanadium uptake and an effect of vanadium treatment on $$^{18}$$F-labeled water movement in a cowpea plant by positron emitting tracer imaging system (PETIS)

Furukawa, Jun*; Yokota, Harumi*; Tanoi, Keitaro*; Ueoka, Shiori*; Matsuhashi, Shimpei; Ishioka, Noriko; Watanabe, Satoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; Ito, Takehito*; et al.

Journal of Radioanalytical and Nuclear Chemistry, 249(2), p.495 - 498, 2001/08

 Times Cited Count:14 Percentile:70.37(Chemistry, Analytical)

We present real time Vanadate (V$$^{5+}$$) uptake imaging in a cowpea plant by Positron Emitting Tracer Imaging System (PETIS). Vanadium-48 was produced by bombarding a Sc foil target with 50 MeV $$alpha$$-particles at Takasaki Ion accelerators for Advanced Radiation Application (TIARA) AVF cyclotron. Then $$^{48}$$V was added to the culture solution to investigate the V distribution in a cowpea plant. The real time uptake of the $$^{48}$$V was monitored by PETIS. We measured the distribution of $$^{48}$$V in a whole plant after 3, 6 and 20 hours of V treatment by Bio-imaging Analyzer System (BAS). After the 20 hour treatment, vanadate was detected at the up-ground part of the plant. To know the effect of V uptake on plant activity, $$^{18}$$F-labeled water uptake was analyzed by PETIS. When a cowpea plant was treated with V for 20 hours before $$^{18}$$F-labeled water uptake experiment, the total amount of $$^{18}$$F-labeled water absorption was drastically desreased. Results suggest the inhibition of water uptake was mainly caused by the vanadate already moved to the up-ground part of the plant.

Journal Articles

$$^{18}$$F used as tracer to study water uptake and transport imaging of a cowpea plant

Nakanishi, Tomoko*; Tanoi, Keitaro*; Yokota, Harumi*; Kang, D.-J.*; Ishii, Ryuichi*; Ishioka, Noriko; Watanabe, Satoshi; Osa, Akihiko; Sekine, Toshiaki; Matsuhashi, Shimpei; et al.

Journal of Radioanalytical and Nuclear Chemistry, 249(2), p.503 - 507, 2001/08

We present the water uptake ability of cowpea ($$it {Vigna unguliculata Walp.}$$) which has been regarded as one of the most drought resistant species among the pulse crops. It has been suggested that in the lower part of the stem, parenchymatous tissue for storing water had been developed for the function of deought resistance. We confirmed that in this tissue, water amount was high compared to the other stems by neutron radiography. Then the water uptake manner was measured by positron emitting tracer imaging system (PETIS) using $$^{18}$$F labeled water produced by a cyclotron. Comparing the water uptake manner of cowpea plant with that of common bean, cowpea plant was found to maintain high water uptake activity after drying treatment, suggesting the high drought resistant character.

Journal Articles

Study on plant physiological functions using positron emitting tracer

Chino, Mitsuo*; Kume, Tamikazu

Nihon Genshiryoku Gakkai-Shi, 41(10), p.35 - 36, 1999/10

no abstracts in English

Journal Articles

Application of positron emitting tracer imaging system (PETIS) for plant research

Kume, Tamikazu

Radioisotopes, 48(1), p.37 - 47, 1999/00

no abstracts in English

Journal Articles

Positron imaging for plants; Status of study using PETIS

Kume, Tamikazu

Hoshasen To Sangyo, (80), p.4 - 5, 1998/00

no abstracts in English

Journal Articles

Positron emitting tracer in plants

Kume, Tamikazu; Fujimura, Takashi; Ishioka, Noriko; Uchida, Hiroshi*; Tsuji, Atsunori*

Nature, 389(6649), 1 Pages, 1997/09

no abstracts in English

Oral presentation

Visualization of source and sink strength in ${it Arabidopsis thaliana}$ using $$^{11}$$CO$$_{2}$$ and a positron-emitting tracer imaging system (PETIS)

Fujimaki, Shu; Koyanagi, Atsushi*; Kawachi, Naoki; Suzui, Nobuo; Yin, Y.-G.; Ishii, Satomi; Shimada, Hiroaki*

no journal, , 

We developed an experimental system to evaluate photosynthetic activities of ${it Arabidopsis thaliana}$ individuals using $$^{11}$$CO$$_{2}$$ tracer gas and a positron-emitting tracer imaging system (PETIS). Two petri dishes each containing nine Arabidopsis plants (16 days or 20 days after sown) were placed into an airtight cabinet, and approximately 40 MBq of $$^{11}$$CO$$_{2}$$ was introduced with normal air. The gas in the cabinet was flushed out after 10 minutes of contact, then the dishes were mounted on the center of field of view of the imaging system, and the dynamic image of carbon distribution in the plants was acquired for 1 hour. As a result, the rates of carbon fixation and of carbon translocation in the respective individuals were estimated through quantitative analysis of the image data. Furthermore, the source and sink strength were visualized as a pixel-by-pixel map. Our method is valuable for quantitative analysis of effects of specific genes or substances on photoassimilation and partitioning.

15 (Records 1-15 displayed on this page)
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