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Synthesis, crystal structure, local structure, and magnetic properties of polycrystalline and single-crystalline Ce$$_2$$Pt$$_6$$Al$$_{15}$$

太田 玖吾*; 渡部 悠貴*; 芳賀 芳範; Iesari, F.*; 岡島 敏浩*; 松本 裕司*

Symmetry (Internet), 15(8), p.1488_1 - 1488_13, 2023/07

Polycrystalline and single crystalline Ce$$_2$$Pt$$_6$$Al$$_{15}$$ samples were synthesized and characterized using EPMA, XRD, XAS and magnetization measurements. The valence of Ce in this compound is trivalent, in agreement with magnetic susceptibility and XAS spectra. The analysis of EXAFS spectra indicates the formation of a honeycomb lattice rather than the disordered hexagonal structure.


Deformation mechanism of a strong and ductile maraging steel investigated using ${it in situ}$ X-ray synchrotron diffraction

Li, H.*; Liu, Y.*; Zhao, W.*; Liu, B.*; 冨永 亜希; 菖蒲 敬久; Wei, D.*

International Journal of Plasticity, 165, p.103612_1 - 103612_20, 2023/06

 被引用回数:0 パーセンタイル:0.01(Engineering, Mechanical)



Data processing and visualization of X-ray computed tomography images of a JOYO MK-III fuel assembly

Tsai, T.-H.; 佐々木 新治; 前田 宏治

Journal of Nuclear Science and Technology, 60(6), p.715 - 723, 2023/06

 被引用回数:1 パーセンタイル:49.42(Nuclear Science & Technology)

A method for processing and visualizing X-ray computed tomography (CT) images of a fuel assembly is developed and applied to a JOYO MK-III fuel assembly. The method provides vertical-section-like images to observe the spatial distribution of CT values in fuel pins and also supplies images that show the relationship between the linear heat rate (LHR) and radial CT-value distribution. In addition, an attempt to analyze the radial cracks in the CT images is proposed, and the results demonstrate the correlation between LHR and the radial cracks.


A Study on convection in molten zone of aluminum alloy during Fe/Al resistance spot welding

伊與田 宗慶*; 松田 朋己*; 佐野 智一*; 茂田 正哉*; 菖蒲 敬久; 湯本 博勝*; Koyama, Takahisa*; Yamazaki, Hiroshi*; 仙波 泰徳*; 大橋 治彦*; et al.

Journal of Manufacturing Processes, 94, p.424 - 434, 2023/05

 被引用回数:1 パーセンタイル:84.05(Engineering, Manufacturing)

Aluminum alloys are increasingly being applied to automobile bodies to reduce the weight of automobiles. In joining steel materials and aluminum alloys using resistance spot welding (RSW), it is important to control the state of intermetallic compounds due to the temperature at the joining interface. In other words, in RSW of Fe/Al dissimilar materials, it is necessary to clarify the heating and cooling phenomena of the interface temperature during joining. Although the convection behavior of the molten aluminum alloy is thought to influence the temperature distribution at the joining interface, there are no studies that have directly observed this phenomenon. In this study, convection in molten zone of aluminum alloy during RSW of steel and aluminum alloy is discussed. Direct observations were attempted in order to clarify the convection behavior of the molten aluminum alloy in RSW of steel and aluminum alloy. The main feature of this experiment is that a real-scale test piece and an RSW apparatus used in actual production were used to observe convection during actual production. The observation experiments were conducted using synchrotron radiation X-ray at SPring-8. During welding, the specimens were irradiated with synchrotron radiation X-ray, and convection was observed from the behavior of tracer particles placed on the specimens. As a results, three types of convection were observed: radial outward convection from the center of the molten zone at the joining interface, convection from the edge of the molten zone toward its center, and weak circulating convection at the edge of the molten zone. And, small convection velocities were generated at the edge of the molten zone. Furthermore, the convection velocity inside the molten zone was calculated to be approximately 1.75 m/s. In addition, it was shown that there is a correlation between convection behavior and the shape of the molten zone.


Relationship between internal stress distribution and microstructure in a suspension-sprayed thermal barrier coating with a columnar structure

山崎 泰広*; 篠宮 啓介*; 奥村 忠晴*; 鈴木 賢治*; 菖蒲 敬久; 中村 唯我*

Quantum Beam Science (Internet), 7(2), p.14_1 - 14_12, 2023/05

The suspension plasma spray (SPS) technique has attracted attention because of its various microstructures, which can be achieved by mixing submicron spray particles with a solvent to form a suspension. Thermal barrier coatings (TBCs) with a columnar structure, which might achieve high strain tolerance, can be obtained using the SPS technique. In this study, the internal stress distribution of the SPS-TBC with different columnar structures was evaluated by hybrid measurement using high-energy synchrotron X-ray diffraction analysis and laboratory low-energy X-rays. The effect of microstructure on the internal stress distribution of the SPS-TBC was discussed on the basis of the experimental results. In addition, the in-plane internal stress was decreased by decreasing the column diameter. The thin columnar microstructure of the SPS-TBC has superior strain tolerance. The internal stresses in the column of the SPS-TBC are periodic decrements caused by stress relaxation in porous layers.


Selective adsorption of Pd(II) over Ag(I) in nitric acid solutions using nitrogen-donor-type adsorbents

鈴木 智也*; 大坪 右京*; 尾形 剛志*; 塩飽 秀啓; 小林 徹; 矢板 毅; 松岡 光昭*; 村山 憲弘*; 成田 弘一*

Separation and Purification Technology, 308, p.122943_1 - 122943_7, 2023/03

 被引用回数:1 パーセンタイル:26.15(Engineering, Chemical)

主にAgを含む使用済み製品からPd金属をリサイクルするために硝酸抽出が行われており、ほとんどのPd残留物はAg(I)含有溶液から分離される。しかし、このAg(I)溶液中には少量のPd(II)が残存していることが多い。そのため、硝酸溶液中のPd(II)とAg(I)を分離することは、Pdのリサイクルを効率的に進めるために必要不可欠である。本研究では、アミン(R-Amine),イミノ二酢酸(R-IDA),ピリジン(R-Py),ビスピコリルアミン(R-BPA)で機能化した4種類の窒素ドナー型吸着材を用いて硝酸溶液中のPd(II), Ag(I)を分離する研究を実施した。R-Amine,R-IDA,R-Pyは硝酸溶液からAg(I), Cu(II), Ni(II), Fe(III)を選択的に吸着したが、R-AmineはPdの吸着効率が低いことが判明した。一方、R-BPAはPd(II), Ag(I), Cu(II)を全濃度において90%以上吸着することができた。フーリエ変換赤外分光法及び広域X線吸収微細構造法(EXAFS)による吸着金属イオンの構造解析から、窒素ドナー型吸着剤の分離機構を明らかにした。R-IDA, R-Py, R-BPAへのPd(II)吸着は官能基(それぞれイミノ二酢酸,ピリジン,ビスピコリルアミン)へのPd(II)配位を介して起こり、R-Amineへの吸着はNO$$_{3}$$$$^{-}$$と[Pd(NO$$_{3}$$)$$_{4}$$]$$^{2-}$$のアニオン交換を介して起こることがわかった。R-IDA,R-Py,R-BPAは、配位性吸着機構により、高いPd(II)吸着挙動を示すことが明らかとなった。塩酸(5.0M)およびチオ尿素(0.1M)溶離液は、それぞれR-IDAから83%およびR-Pyから95%のPd(II)を脱離させた。R-Pyは吸着選択性と脱着効率から最も効果的なPd(II)吸着剤だった。


Insight on the mechanical properties of hierarchical porous calcium-silicate-hydrate pastes according to the Ca/Si molar ratio using ${it in situ}$ synchrotron X-ray scattering and nanoindentation test

Im, S.*; Jee, H.*; Suh, H.*; 兼松 学*; 諸岡 聡; Choe, H.*; 西尾 悠平*; 町田 晃彦*; Kim, J.*; Lim, S.*; et al.

Construction and Building Materials, 365, p.130034_1 - 130034_18, 2023/02

 被引用回数:3 パーセンタイル:97.57(Construction & Building Technology)

Nanocrystalline calcium-silicate-hydrate (C-S-H) is a typical heterogeneous material with a multiscale structure spanning a wide length scale from angstrom to micrometer, and whose structure is determined by the Ca/Si ratio. In this study, we directly applied compressive loads on synthetic C-S-H pastes with Ca/Si ratios of 0.6-1.2 and investigated their mechanical properties using the elastic modulus calculated at three length scale levels (i.e., angstrom to nanometer, micrometer, and millimeter) via in-situ synchrotron X-ray scattering, nanoindentation tests, and strain gauges, respectively. Further, $$^{29}$$Si nuclear magnetic resonance spectroscopy was conducted on the C-S-H pastes to elucidate the alterations in the silicate polymerization. The experimental results confirmed the deformation behavior of the C-S-H paste with different Ca/Si ratios under external loading, which was demonstrated to be transferred from the surface of the pastes to particles owing to the presence of multiscale pores.


$$beta$$$$gamma$$、X線同時解析による迅速・高感度放射性核種分析法の開発(委託研究); 令和3年度英知を結集した原子力科学技術・人材育成推進事業

廃炉環境国際共同研究センター; 日本分析センター*

JAEA-Review 2022-037, 118 Pages, 2023/01


日本原子力研究開発機構(JAEA)廃炉環境国際共同研究センター(CLADS)では、令和3年度英知を結集した原子力科学技術・人材育成推進事業(以下、「本事業」という)を実施している。本事業は、東京電力ホールディングス株式会社福島第一原子力発電所の廃炉等を始めとした原子力分野の課題解決に貢献するため、国内外の英知を結集し、様々な分野の知見や経験を、従前の機関や分野の壁を越えて緊密に融合・連携させた基礎的・基盤的研究及び人材育成を推進することを目的としている。平成30年度の新規採択課題から実施主体を文部科学省からJAEAに移行することで、JAEAとアカデミアとの連携を強化し、廃炉に資する中長期的な研究開発・人材育成をより安定的かつ継続的に実施する体制を構築した。本研究は、研究課題のうち、令和2年度に採択された「$$beta$$$$gamma$$、X線同時解析による迅速・高感度放射性核種分析法の開発」の令和3年度の研究成果について取りまとめたものである。本研究は、燃料デブリ・廃棄物中放射性核種の迅速分析の実現を目指し、多重$$gamma$$線検出法などの最新計測システムを整備し、スペクトル定量法(Spectral Determination Method: 以下、「SDM法」という)を開発する。令和3年度の研究においては、令和2年度に引き続き、$$gamma$$線測定装置を整備し、Ge検出器、CeBr$$_{3}$$検出器、NaI検出器からなる各計測システムを完成させた。また、高速データ収集システムを整備し、1次元及び同時計数データ取得を可能にした。SDM法開発においては、標準線源を測定およびシミュレーション計算を活用し、$$gamma$$線シングルス、多重$$gamma$$線測定、$$beta$$線スペクトルの標準スペクトルを生成し、全40核種のスペクトルデータベースを整備した。また、$$beta$$(+X)線、$$gamma$$線、多重$$gamma$$線スペクトルを統合解析するSDM法(SDM-BG法及びSDM-BGG法)を開発した。SDM法の高精度化のため、畳み込みニューラルネットワーク(CNN)を用いて、複数核種(Co-60、Cs-134、Cs-137、Eu-152等7核種)の核種識別が可能な機械学習モデルを構築した。


Effect of magnesium silicate hydrate (M-S-H) formation on the local atomic arrangements and mechanical properties of calcium silicate hydrate (C-S-H); In situ X-ray scattering study

Kim, G.*; Im, S.*; Jee, H.*; Suh, H.*; Cho, S.*; 兼松 学*; 諸岡 聡; 小山 拓*; 西尾 悠平*; 町田 晃彦*; et al.

Cement and Concrete Research, 159, p.106869_1 - 106869_17, 2022/09

 被引用回数:3 パーセンタイル:63.89(Construction & Building Technology)

This study explored the effect of M-S-H formation on the local atomic arrangements and mechanical properties of C-S-H. The elastic moduli of the samples were calculated using shifted atomic distances (r) and d-spacings (d) acquired by applying an external load on the pastes during X-ray scattering experiments. The experimental results indicated that the crystal structure of C-S-H remained intact with MgCl$$_{2}$$ addition. At the highest Mg/Si ratio (Ca/Si = 0.6, Mg/Si = 0.2), change in the dominant phase occurred from C-S-H to M-S-H because the low pH environment hindered the formation of C-S-H and facilitated the formation of M-S-H. The elastic modulus decreased with increasing Mg/Si ratio up to 0.1 owing to both C-S-H destabilization and low M-S-H content in the samples. Conversely, the elastic modulus increased in the paste synthesized with the highest Mg/Si ratio because considerable M-S-H had formed, which exhibited a higher elastic modulus than C-S-H.


Micro- to nano-scale areal heterogeneity in pore structure and mineral compositions of a sub-decimeter-sized Eagle Ford Shale

Wang, Q.*; Hu, Q.*; Zhao, C.*; Yang, X.*; Zhang, T.*; Ilavsky, J.*; Kuzmenko, I.*; Ma, B.*; 舘 幸男

International Journal of Coal Geology, 261, p.104093_1 - 104093_15, 2022/09

 被引用回数:3 パーセンタイル:71.11(Energy & Fuels)

To understanding the spatial heterogeneity of mineral and pore structure variations in fine-grained shale, microscale X-ray fluorescence (micro-XRF) mapping, (ultra-) small-angle X-ray scattering [(U)SAXS] and wide-angle X-ray scattering were applied for two samples from a piece of Eagle Ford Shale in South Texas. Thin section petrography and field emission-scanning electron microscopy, X-ray diffraction (XRD), total organic carbon, and pyrolysis were also utilized to investigate the potential spatial heterogeneity of pore types, mineral and organic matter compositions for both samples. Overall, the siliceous-carbonate mineral contents in these carbonate-rich Eagle Ford Shale vary between laminations at mm scales. By analyzing six selected sub-samples on each of two samples with X-ray scattering and XRD techniques, nm-sized pores are mainly interparticle ones in the higher calcite regions, where the porosity is also relatively lower, while the lower calcite regions consist of both interparticle and intraparticle pore types with higher porosity. Finally, the micro-XRF and (U)SAXS are combined to generate porosity distribution maps to provide more insights about its heterogeneity related to the laminations and fractures at our observational scales.


Study on the relation between the crystal structure and thermal stability of FeUO$$_{4}$$ and CrUO$$_{4}$$

秋山 大輔*; 日下 良二; 熊谷 友多; 中田 正美; 渡邉 雅之; 岡本 芳浩; 永井 崇之; 佐藤 修彰*; 桐島 陽*

Journal of Nuclear Materials, 568, p.153847_1 - 153847_10, 2022/09

 被引用回数:2 パーセンタイル:72.55(Materials Science, Multidisciplinary)



$$beta$$$$gamma$$、X線同時解析による迅速・高感度放射性核種分析法の開発(委託研究); 令和2年度英知を結集した原子力科学技術・人材育成推進事業

廃炉環境国際共同研究センター; 日本分析センター*

JAEA-Review 2021-060, 105 Pages, 2022/03


日本原子力研究開発機構(JAEA)廃炉環境国際共同研究センター(CLADS)では、令和2年度英知を結集した原子力科学技術・人材育成推進事業(以下、「本事業」という)を実施している。本事業は、東京電力ホールディングス株式会社福島第一原子力発電所の廃炉等をはじめとした原子力分野の課題解決に貢献するため、国内外の英知を結集し、様々な分野の知見や経験を、従前の機関や分野の壁を越えて緊密に融合・連携させた基礎的・基盤的研究及び人材育成を推進することを目的としている。平成30年度の新規採択課題から実施主体を文部科学省からJAEAに移行することで、JAEAとアカデミアとの連携を強化し、廃炉に資する中長期的な研究開発・人材育成をより安定的かつ継続的に実施する体制を構築した。本研究は、研究課題のうち、令和2年度に採択された「$$beta$$$$gamma$$、X線同時解析による迅速・高感度放射性核種分析法の開発」の令和2年度の研究成果について取りまとめたものである。本研究は、燃料デブリ・廃棄物中放射性核種の迅速分析の実現を目指し、多重$$gamma$$線検出法などの最新計測システムを整備し、これを用いた測定、また放射線シミュレーション計算により$$beta$$線、X線を含む核種毎のスペクトルデータベースを構築し、これらを統合解析するスペクトル定量解読法(Spectral Determination Method: SDM法)を開発することにより、多核種同時定量を可能にし、化学分離プロセスを軽減することを目的とする。


Influence of distant scatterer on air kerma measurement in the evaluation of diagnostic X-rays using Monte Carlo simulation

富永 正英*; 永安 結花里*; 佐々木 幹治*; 古田 琢哉; 林 裕晃*; 笈田 将皇*; 西山 祐一*; 芳賀 昭弘*

Radiological Physics and Technology, 14(4), p.381 - 389, 2021/12



Temperature effects on local structure, phase transformation, and mechanical properties of calcium silicate hydrates

Im, S.*; Jee, H.*; Suh, H.*; 兼松 学*; 諸岡 聡; 小山 拓*; 西尾 悠平*; 町田 晃彦*; Kim, J.*; Bae, S.*

Journal of the American Ceramic Society, 104(9), p.4803 - 4818, 2021/09

 被引用回数:8 パーセンタイル:67.73(Materials Science, Ceramics)

This study aims to elucidate the effect of heating on the local atomic arrangements, structure, phase transformation, and mechanical properties of synthesized calcium-silicate-hydrate (C-S-H). The alteration in the atomic arrangement of the synthesized C-S-H (Ca/Si = 0.8) and the formation of crystalline phases that occurred in three distinct transformation stages of dehydration (105-200 $$^{circ}$$C), decomposition (300-600 $$^{circ}$$C), and recrystallization (700-1000 $$^{circ}$$C) were investigated via powder X-ray diffraction, $$^{29}$$Si nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. Further, the deformation of the local atomic bonding environment and variations in mechanical properties during the three stages were assessed via pair distribution function analysis based on in-situ total X-ray scattering. The results revealed that the C-S-H paste before heating exhibited a lower elastic modulus in real space than that in the reciprocal space in the initial loading stage because water molecules acted as a lubricant in the interlayer. At the dehydration stage, the strain as a function of external loading exhibited irregular deformation owing to the formation of additional pores induced by the evaporation of free moisture. At the decomposition stage, the structural deformation of the main d-spacing (d $$approx$$ 3.0 ${AA}$) was similar to that of the real space before the propagation of microcracks. At the recrystallization stage, the elastic modulus increased to 48 GPa owing to the thermal phase transformation of C-S-H to crystalline $$beta$$-wollastonite. The results provide direct experimental evidence of the micro- and nanostructural deformation behavior of C-S-H pastes after exposure to high temperature under external loading.


A Multi-technique tomography-based approach for non-invasive characterization of additive manufacturing components in view of vacuum/UHV applications; Preliminary results

Grazzi, F.*; Cialdai, C.*; Manetti, M.*; Massi, M.*; Morigi, M. P.*; Bettuzzi, M.*; Brancaccio, R.*; Albertin, F.*; 篠原 武尚; 甲斐 哲也; et al.

Rendiconti Lincei. Scienze Fisiche e Naturali, 32(3), p.463 - 477, 2021/09

 被引用回数:2 パーセンタイル:19.8(Multidisciplinary Sciences)

In this paper, we have studied an additively manufactured metallic component, intended for ultra-high vacuum application, the exit-snout of the MACHINA transportable proton accelerator beam-line. Metal additive manufacturing components can exhibit heterogeneous and anisotropic microstructures. Two non-destructive imaging techniques, X-ray computed tomography and Neutron Tomography, were employed to examine its microstructure. They unveiled the presence of porosity and channels, the size and composition of grains and intergranular precipitates, and the general behavior of the spatial distribution of the solidification lines. While X-ray computed tomography evidenced qualitative details about the surface roughness and internal defects, neutron tomography showed excellent ability in imaging the spatial density distribution within the component. The anisotropy of the density was attributed to the material building orientation during the 3D printing process. Density variations suggest the possibility of defect pathways, which could affect high vacuum performances. In addition, these results highlight the importance of considering building orientation in the design for additive manufacturing for UHV applications.


Structure of the {U$$_{13}$$} polyoxo cluster U$$_{13}$$O$$_{8}$$Cl$$_{x}$$(MeO)$$_{38-x}$$ (x = 2.3, MeO = methoxide)

Fichter, S.*; Radoske, T.*; 池田 篤史

Acta Crystallographica Section E; Crystallographic Communications (Internet), 77(8), p.847 - 852, 2021/08

A new type of polyoxo cluster complex that contains thirteen uranium atoms, {U$$_{13}$$}, was synthesised and characterised as [U$$_{13}$$($$mu$$$$_{4}$$-O$$_{rm oxo}$$)$$_{8}$$($$mu$$$$_{4}$$-O$$_{rm MeO}$$)$$_{2}$$($$mu$$$$_{2}$$-O$$_{rm MeO}$$)$$_{24}$$Cl$$_{x}$$(O$$_{rm MeO}$$)$$_{12-x}$$] (x = 2.3, MeO = methoxide) (I). The complex crystallises from methanol containing tetravalent uranium (U$$^{rm IV}$$) with a basic organic ligand. The characterised {U$$_{13}$$} polyoxo cluster complex possesses a single cubic uranium polyhedron at the centre of the cluster core. The observed shortening of U-O bonds, together with BVS calculations and the overall negative charge (2-) of (I), suggests that the central uranium atom in (I), that forms the single cubic polyhedron, is presumably oxidised to the pentavalent state (U$$^{rm V}$$) from the original tetravalent state (U$$^{rm IV}$$). Complex I is, hence, the first example of a polyoxo cluster possessing a single cubic coordination polyhedron of U$$^{rm V}$$.


Feasibility study of magnetic-domain observation by means of non-resonant magnetic X-ray diffraction

稲見 俊哉*; 菖蒲 敬久; 石井 賢司*

IEEE Transactions on Magnetics, 57(3, Part2), p.6400105_1 - 6400105_5, 2021/03

 被引用回数:0 パーセンタイル:0(Engineering, Electrical & Electronic)

Although the observation of magnetic domains well below the surface of a sample appears to be relatively easy, in reality, magnetic domains inside metallic samples have not been investigated in detail due to a lack of adequate techniques. Non-resonant magnetic X-ray diffraction (NRMXRD) is a candidate for such 3-D magnetic microscopy. A relatively small focus size of less than 100 nm and a relatively long attenuation length of more than 100 $$mu$$m can be obtained by using high-energy synchrotron X-rays. However, NRMXRD has a disadvantage in that the magnetic contrast is low. It is known that the magnetic contrast in NRMXRD increases with increasing the total polarization of the incident X-rays. In this feasibility study, we carried out NRMXRD experiments on a metallic iron sample to examine whether the magnetic contrast can be sufficiently enhanced even for high-energy X-rays.


Development of experimental technology for simulated fuel-assembly heating to address core-material-relocation behavior during severe accident

阿部 雄太; 山下 拓哉; 佐藤 一憲; 中桐 俊男; 石見 明洋

Journal of Nuclear Engineering and Radiation Science, 6(2), p.021113_1 - 021113_9, 2020/04

The authors are developing an experimental technology for simulating severe accident (SA) conditions using simulate fuel material (ZrO$$_{2}$$) that would contribute, not only to Fukushima Daiichi (1F) decommissioning, but also to enhance the safety of worldwide existing and future nuclear power plants through clarification of accident progression behavior. Nontransfer (NTR) type plasma, which has been in practical use with a large torch capacity as high as 2 MW, has the potential to heat subject materials to very high temperatures without selecting the target to be heated. When simulating 1F with SA code, the target of this core-material-melting and relocation (CMMR) experiment was to confirm that NTR plasma has a sufficient heating performance realizing large temperature gradients ($$>$$ 2000 K/m) expected under 1F conditions. The authors selected NTR-type plasma-heating technology that has the advantage of continuous heating in addition to its high-temperature level. The CMMR-2 experiments were carried out in 2017 applying the improved technology (higher heating power and controlled oxygen concentration). The CMMR-2 experiment adopted a 30-min heating period, wherein the power was increased to a level where a large temperature gradient was expected at the lower part of the core under actual 1F accident conditions. Most of the control blade and channel box migrated from the original position. After heating, the simulated fuel assembly was measured by X-ray computed tomography (CT) technology and by electron probe micro-analyzer (EPMA). CT pictures and elemental mapping demonstrated its excellent performance with rather good precision. Based on these results, an excellent perspective, in terms of applicability of the NTR-type plasma-heating technology to the SA experimental study, was obtained.


Segmental motions of proteins under non-native states evaluated using quasielastic neutron scattering

藤原 悟*; 松尾 龍人*; 杉本 泰伸*; 柴田 薫

Journal of Physical Chemistry Letters (Internet), 10(23), p.7505 - 7509, 2019/12

 被引用回数:4 パーセンタイル:24.74(Chemistry, Physical)

無秩序なポリペプチド鎖のダイナミクスの特性評価は、本質的に無秩序状態なタンパク質およびフォールディングプロセスに関連する非ネイティブ状態下のタンパク質の挙動を解明するために必要である。本研究では、小角X線散乱測定データと動的光散乱測定データと組み合わせて準弾性中性子散乱測定データから、タンパク質のセグメント運動と分子全体の拡散および局所側鎖運動を評価する方法を独自に開発した。そしてこの方法を、非フォールディング状態およびメルトグロビュール(MG)状態のタンパク質RNase Aに適用し、セグメント運動から生じる拡散係数を評価し、非フォールディング状態とMG状態で異なる値をとることを明らかにした。またこの方法で得られた値は、蛍光現象を用いた別の測定技術を使用して得られた値と一致していることも確認できた。これらの研究成果は、この方法の、さまざまな無秩序状態でのタンパク質の挙動を特徴付ける実行可能性だけでなく、有用性も示している。


Dynamic properties of human $$alpha$$-synuclein related to propensity to amyloid fibril formation

藤原 悟*; 河野 史明*; 松尾 龍人*; 杉本 泰伸*; 松本 友治*; 成田 哲博*; 柴田 薫

Journal of Molecular Biology, 431(17), p.3229 - 3245, 2019/08

 被引用回数:11 パーセンタイル:46.92(Biochemistry & Molecular Biology)


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