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Inagaki, Atsushi*; Kanda, Manabu*; Ahmad, N. H.*; Yagi, Ayako*; Onodera, Naoyuki; Aoki, Takayuki*
Boundary-Layer Meteorology, 164(2), p.161 - 181, 2017/08
Times Cited Count：30 Percentile：75.23(Meteorology & Atmospheric Sciences)The applicability of outer-layer scaling is examined by numerical simulation of a developing neutral boundary layer over a realistic building geometry of Tokyo. Large-eddy simulations are carried out over a large computational domain 19.2 km 
4.8 km 1 km, with a fine grid spacing (2 m) using the lattice-Boltzmann method with massively parallel graphics processing units. Results from simulations show that outer-layer features are maintained for turbulence statistics in the upper part of the boundary layer, as well as the width of predominant streaky structures throughout the entire boundary layer. This is caused by the existence of very large streaky structures extending throughout the entire boundary layer, which follow outer-layer scaling with a self-preserving development. We assume the top-down mechanism in the physical interpretation of results.
Affrida, A. H.*; Ahmad, Z.*; Idris, N. A.*; Mohd Randzan, N.*; Idris, N.*; Basiran, M. N.*; Tanaka, Atsushi; Shikazono, Naoya; Ono, Yutaka; Hase, Yoshihiro
no journal, ,
Mutation induction by ion beam provides an alternative for improvement of orchids. The protocorm-like bodies (PLBs) of three orchid species; 
, 
and 
were irradiated with 
C
ions, accelerated by AVF Cyclotron at Japanese Atomic Energy Agency (JAEA), Japan, at various doses ranging from 0 to 12.0 Gray (Gy). Since no morphological change was detected, Amplified Fragment Length Polymorphism (AFLP) technique was used to analyse the irradiated orchids. This PCR based DNA fingerprinting technique involves digestion of DNA with restriction enzymes, ligation of adapter and selective amplification using primer with one (pre-amplification) and three (selective amplification) arbitrary nucleotides. The fingerprinting of the control and mutant lines were compared in order to detect their genetic difference. Polymorphic fragments ranging from 250 to 1200 bp length were detected.
