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Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Takeda, Shigeo*; Mukai, Sadaatsu*; Fukazawa, Hiroshi; Littrell, K. C.*; Sasaki, Yoshihiro*; Akiyoshi, Kazunari*
Journal of Physical Chemistry B, 120(46), p.11996 - 12002, 2016/11
Times Cited Count:10 Percentile:27.16(Chemistry, Physical)The detailed structure of a nanogel formed by self-association of cholesterol-bearing pullulans (CHP) was determined by contrast variation small-angle neutron scattering. The decomposition of scattering intensities into partial scattering functions of each CHP nanogel component, i.e., pullulan, cholesterol, and the cross-term between the pullulan and the cholesterol allows us to investigate the internal structure of the nanogel. The effective spherical radius of the skeleton formed by pullulan chains was found to be about 8.1 nm. In the CHP nanogel, there are about 19 cross-linking points where a cross-linking point is formed by aggregation of trimer cholesterol molecules with the spatially inhomogeneous distribution of the mass fractal dimension of 2.6. The average radius of the partial chains can also be determined to be 1.7 nm. As the result, the complex structure of the nanogels is coherently revealed at the nanoscopical level.
Sekine, Yurina; Fukazawa, Hiroshi; Endo, Hitoshi*; Iwase, Hiroki*; Mukai, Sadaatsu*; Littrell, K. C.*; Fernandez-Baca, J. A.*; Akiyoshi, Kazunari*
no journal, ,
Cholesterol-bearing pullulan (CHP) spontaneously forms stable nanogel of ca. 30 nm in size in water. Because the CHP nanogels can trap various proteins spontaneously and release them in their native form to environment, they are valuable for use in protein delivery systems. The aim of this study is to investigate the distribution and position of cross-linking domain of nanogels by means of contrast variation small-angle neutron scattering (CV-SANS). We had SANS measurement of the CHP nanogel dispersed in complex solvent of DO/HO to clarify the microscopic structure of cross-linking domain. The result shows that the particle radius of the pullulan and the cholesterol are 8.09 and 6.31 nm, respectively. The dispersion of the cross-linking domains might have important implications for the interactions of nanogels with other molecules.