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Journal Articles

Magnetically navigated protein transduction in vivo using iron oxide-nanogel chaperone hybrid

Kawasaki, Riku*; Sasaki, Yoshihiro*; Nishimura, Tomoki*; Katagiri, Kiyofumi*; Morita, Keiichi*; Sekine, Yurina; Sawada, Shinichi*; Mukai, Sadaatsu*; Akiyoshi, Kazunari*

Advanced Healthcare Materials, 10(9), p.2001988_1 - 2001988_8, 2021/05

 Times Cited Count:6 Percentile:61.24(Engineering, Biomedical)

Systems for "protein transduction", the intracellular delivery of functional proteins, are needed to address the deliverability challenges of protein therapy, but protein transfer in vivo remains difficult. In this study, we have developed a magnetically induced in vivo protein transfection system using a magnetic nanogel chaperone (MC) composed of iron oxide nanoparticles and polysaccharide nanogels. Experiments using an oral cancer model have shown that this MC system is useful for cancer treatment.

Journal Articles

Nanoscopic structural investigation of physically cross-linked nanogels formed from self-associating polymers

Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Takeda, Shigeo*; Mukai, Sadaatsu*; Fukazawa, Hiroshi; Littrell, K. C.*; Sasaki, Yoshihiro*; Akiyoshi, Kazunari*

Journal of Physical Chemistry B, 120(46), p.11996 - 12002, 2016/11

 Times Cited Count:10 Percentile:27.16(Chemistry, Physical)

The detailed structure of a nanogel formed by self-association of cholesterol-bearing pullulans (CHP) was determined by contrast variation small-angle neutron scattering. The decomposition of scattering intensities into partial scattering functions of each CHP nanogel component, i.e., pullulan, cholesterol, and the cross-term between the pullulan and the cholesterol allows us to investigate the internal structure of the nanogel. The effective spherical radius of the skeleton formed by pullulan chains was found to be about 8.1 nm. In the CHP nanogel, there are about 19 cross-linking points where a cross-linking point is formed by aggregation of trimer cholesterol molecules with the spatially inhomogeneous distribution of the mass fractal dimension of 2.6. The average radius of the partial chains can also be determined to be 1.7 nm. As the result, the complex structure of the nanogels is coherently revealed at the nanoscopical level.

Oral presentation

Nanoscopic structure of physically cross-linked nanogels investigated by small-angle neutron scattering

Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Takeda, Shigeo*; Mukai, Sadaatsu*; Fukazawa, Hiroshi; Sasaki, Yoshihiro*; Akiyoshi, Kazunari*

no journal, , 

Cholesterol-bearing pullulan (CHP) forms stable nanogel of 20 nm in size in water. The CHP nanogels can trap various proteins spontaneously. Owing to their characteristics, nanogels can be used in protein delivery systems, particularly for cancer vaccines. The spatial distribution and detailed structure of the cross-linking points are important factor determining their chemical and physical functions. The aim of this study is to investigate the structure of the nanogels by means of contrast variation small-angle neutron scattering (CV-SANS), which enables us to determine quantitative complex structure at nanometer scale. In this presentation, we will discuss the detailed structure of the CHP nanogel.

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