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Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.
Journal of Radiation Research, 48(3), p.247 - 253, 2007/05
Times Cited Count：17 Percentile：47.71(Biology)To carry out the radio-microsurgery study using silkworm, 
, we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon (
C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.
, after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strainKiguchi, Kenji*; Shirai, Koji*; Sakata, Toshiyuki*; Fukamoto, Kana; Kakizaki, Takehiko; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 117, 2007/02
no abstracts in English
Sakata, Toshiyuki*; Shirai, Koji*; Kiguchi, Kenji*; Fukamoto, Kana; Kakizaki, Takehiko; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
Fukamoto, Kana; Sakata, Toshiyuki*; Shirai, Koji*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; Hara, Takamitsu*; Suzuki, Michiyo*; et al.
no journal, ,
Silkworm is an experimental insect good to investigate developmental biology or cell differentiation. Knobbed mutant is a quite unique and important model of cell differentiation, in that cells in the knob region consist of abnormally proliferated and stratified cells. In this study, the new application of irradiation with heavy ion microbeam for the first instar silkworm larvae was developed to clarify that when and where the knob mutant would form abnormal proliferation of epidermal cells. The holed aluminum plates were designed to fix the first instar larvae of silkworm during irradiation. After carbon ions microbeam irradiation, larvae were reared to evaluate the accuracy of irradiation. The deletion of knob was observed in over 70% of the larvae at fifth instar. The epidermal cells stayed, as it was a monolayer at irradiated region. These results indicate that heavy ion beam irradiation can control the abnormal cell division of epidermis in the knob mutant.
Fukamoto, Kana; Sakata, Toshiyuki*; Shirai, Koji*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; Hara, Takamitsu*; Suzuki, Michiyo; et al.
no journal, ,
no abstracts in English
Sakata, Toshiyuki*; Shirai, Koji*; Tsuchiya, Shiori*; Kiguchi, Kenji*; Fukamoto, Kana; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Sato, Shigeru*
no journal, ,
no abstracts in English
, after heavy-ion irradiation; Detection and identification of the stage-specific markerKobayashi, Satoshi*; Sakata, Toshiyuki*; Tsuchiya, Shiori*; Shirai, Koji*; Kiguchi, Kenji*; Fukamoto, Kana; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
, after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strainShirai, Koji*; Kiguchi, Kenji*; Sakata, Toshiyuki*; Fukamoto, Kana; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
no journal, ,
