DNA gyrase of is characterized as Type II bacterial topoisomerase and its activity is differentially regulated by PprA in vitro

Kota, S.*; Rajpurohit, Y. S.*; Charaka, V. K.*; Sato, Katsuya; Narumi, Issey*; Misra, H, S.*

Extremophiles, 20(2), p.195 - 205, 2016/03

https://doi.org/10.1007/s00792-016-0814-1

Accumulation of cesium and its association with intracellular polyphosphate in

Ueda, Ryoshiro*; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 101, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Genome sequence analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding, 2

Masubuchi, Takashi*; Toguchi, Kazuki*; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Sato, Katsuya; Ono, Yutaka

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 106, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Draft genome sequence of the radioresistant bacterium , isolated from freshwater fish in Japan

Sato, Katsuya; Onodera, Takefumi*; Omoso, Kota*; Takeda-Yano, Kiyoko*; Katayama, Takeshi*; Ono, Yutaka; Narumi, Issey*

Genome Announcements (Internet), 4(1), p.e01631-15_1 - e01631-15_2, 2016/01

https://doi.org/10.1128/genomeA.01631-15

Modification of cesium absorption in plants and microorganisms by the ion-beam breeding technology

Ono, Yutaka; Hase, Yoshihiro; Sato, Katsuya; Nozawa, Shigeki; Narumi, Issey*

Hoshasen To Sangyo, (138), p.17 - 20, 2015/06

no abstracts in English

Development of cesium-accumulating bacteria by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro; Hase, Yoshihiro; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 117, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=149

Molecular analysis of polyphosphate biosynthesis-related genes in

Ueda, Ryoshiro; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 118, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=150

Genome analysis of the high temperature tolerant mutant of USDA110 generated by ion-beam irradiation

Takeda, Kiyoko*; Sato, Katsuya; Narumi, Issey*; Ono, Yutaka; Otsu, Naoko*; Yokoyama, Tadashi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 120, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=152

Benomyl-resistant (Hypocreales: Clavicipitaceae) mutants induced by ion beams

Fitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*

Applied Entomology and Zoology, 50(1), p.123 - 129, 2015/02

https://doi.org/10.1016/j.apsusc.2015.02.144

Interaction of double-stranded DNA with polymerized PprA protein from

Adachi, Motoyasu; Hirayama, Hiroshi; Shimizu, Rumi; Sato, Katsuya; Narumi, Issey*; Kuroki, Ryota

Protein Science, 23(10), p.1349 - 1358, 2014/10

https://doi.org/10.1002/pro.2519

Pleiotropic protein promoting DNA repair A (PprA) is a key protein that facilitates the extreme radioresistance of . To clarify the role of PprA in the radioresistance mechanism, the interaction between recombinant PprA expressed in Escherichia coli with several double-stranded DNAs was investigated. In a gel-shift assay, the band shift of supercoiled pUC19 DNA caused by the binding of PprA showed a bimodal distribution, which was promoted by the addition of 1 mM Mg, Ca, or Sr ions. The dissociation constant of the PprA-supercoiled pUC19 DNA complex, calculated from the relative portions of shifted bands, was 0.6 M with a Hill coefficient of 3.3 in the presence of 1 mM Mg acetate. This indicates that at least 281 PprA molecules are required to saturate a supercoiled pUC19 DNA, which is consistent with the number of bound PprA molecules estimated by the UV absorption of the PprA-pUC19 complex purified by gel filtration. This saturation also suggests linear polymerization of PprA along the dsDNA. On the other hand, the bands of linear dsDNA and nicked circular dsDNA that eventually formed PprA complexes did not saturate, but created larger molecular complexes when the PprA concentration was greater than 1.3 M. This result implies that DNA-bound PprA aids association of the termini of damaged DNAs, which is regulated by the concentration of PprA.