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Higuchi, Kyoko*; Kurita, Keisuke; Sakai, Takuro; Suzui, Nobuo*; Sasaki, Minori*; Katori, Maya*; Wakabayashi, Yuna*; Majima, Yuta*; Saito, Akihiro*; Oyama, Takuji*; et al.
Plants (Internet), 11(6), p.817_1 - 817_11, 2022/03
Times Cited Count：2 Percentile：37.57(Plant Sciences)Genetic diversity in the rate of Fe uptake by plants has not been broadly surveyed among plant species or genotypes, although plants have developed various Fe acquisition mechanisms. We adopted the "Live-autoradiography" technique with radioactive 
Fe to directly evaluate the uptake rate of Fe by barley cultivars from a nutrient solution containing a very low concentration of Fe. Our observations revealed that the ability to acquire Fe from the low Fe solution was not always the sole determinant of tolerance to Fe deficiency among the barley genotypes.
N-labeled nitrogen gas tracer for the imaging of nitrogen fixation in soybean nodulesIshii, Satomi; Igura, Masato*; Yin, Y.-G.; Hung, N. V. P.*; Suzui, Nobuo; Kawachi, Naoki; Koyanagi, Atsushi*; Oyama, Takuji*; Fujimaki, Shu
JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 98, 2014/03
partial pressure on nitrogen fixation in soybean plant using positron-emitting tracerHung, N. V. P.; Ishii, Satomi; Suzui, Nobuo; Kawachi, Naoki; Yin, Y.-G.; Koyanagi, Atsushi; Oyama, Takuji*; Fujimaki, Shu
JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 99, 2014/03
The impacts of oxygen in rhizosphere on the symbiotic nitrogen fixation in real-time was evaluated under various O partial pressure. Soybean nodules were treated with mixed gas containing N labeled with a radioisotope N and various concentrations of O, and the nitrogen fixation activity in the nodules was visualized by the positron-emitting tracer imaging system (PETIS). The results showed that under normal condition the nitrogen fixation ability of soybean plant was higher compared to that of under the other conditions (0% O and 10% O). There was no significant difference in the nitrogen fixation activity between 0% O and 10% O.
N as a tracerHung, N. V. P.*; Watanabe, Shiori*; Ishikawa, Shinji*; Otake, Norikuni*; Sueyoshi, Kuni*; Sato, Takashi*; Ishii, Satomi; Fujimaki, Shu; Oyama, Takuji*
Soil Science and Plant Nutrition, 59(6), p.888 - 895, 2013/12
Times Cited Count：4 Percentile：14.01(Plant Sciences)Oyama, Takuji; Fujikake, Hiroyuki*; Yashima, Hiroyuki*; Tanabata, Sayuri*; Ishikawa, Shinji*; Sato, Takashi*; Nishiwaki, Toshikazu*; Otake, Norikuni*; Sueyoshi, Kuni*; Ishii, Satomi; et al.
Soybean Physiology and Biochemistry, p.333 - 364, 2011/11
no abstracts in English
Ito, Sayuri; Fujimaki, Shu; Kato, Taichi*; Otake, Norikuni*; Sueyoshi, Kuni*; Oyama, Takuji*
Nitrogen assimilation in plants, p.205 - 214, 2010/07
Fujimaki, Shu; Ishii, Satomi; Oyama, Takuji*
Hoshasen To Sangyo, (124), p.32 - 35, 2009/12
The nodule is a symbiotic organ developed on roots of leguminous plants which have been infected with rhizobia, a group of soil bacteria. The plants with nodules can obtain nitrogen nutrition as ammonia converted from nitrogen gas in the air by rhizobia in the nodules. This function, "symbiotic nitrogen fixation", plays a fundamental role in the food production in the world. Recently, we reported that we have successfully visualized the symbiotic nitrogen fixation in soybean plants non-invasively using a positron-emitting tracer imaging system (PETIS) and nitrogen-13-labelled nitrogen gas tracer. In this article, this study is described with the scientific and social backgrounds.
N-labeled nitrogen gas tracer and the imaging of nitrogen fixation in soybean nodulesIshii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 106, 2009/12
N-labeled nitrogen gasIshii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
Soil Science and Plant Nutrition, 55(5), p.660 - 666, 2009/10
Times Cited Count：15 Percentile：40.97(Plant Sciences)N-labeled nitrogen gas for imaging of nitrogen fixation by soybean noduleIshii, Satomi; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 111, 2008/11
Oyama, Takuji*; Sueyoshi, Kuni*; Otake, Norikuni*; Ito, Sayuri*; Ishibashi, H.*; Hara, T.*; Kimura, T.*; Matsuhashi, Shimpei; Fujimaki, Shu; Suzui, Nobuo; et al.
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 122, 2007/02
Mori, Satoshi*; Nakanishi, Tomoko*; Hayashi, Hiroaki*; Oyama, Takuji*; Uchida, Hiroshi*; Matsuhashi, Shimpei; Sekine, Toshiaki
Radioisotopes, 50(9), p.408 - 418, 2001/09
no abstracts in English
NIshii, Satomi; Otake, Norikuni*; Ishioka, Noriko; Suzui, Nobuo; Fujimaki, Shu; Matsuhashi, Shimpei; Oyama, Takuji*
no journal, ,
no abstracts in English
Ishii, Satomi; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Ito, Sayuri; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
no journal, ,
no abstracts in English
N labeled nitrogen gas for imaging of nitrogen fixation by soybean noduleIshii, Satomi; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Fujimaki, Shu; Otake, Norikuni*; Oyama, Takuji*
no journal, ,
no abstracts in English
Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Kawachi, Naoki; Ishioka, Noriko; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
no journal, ,
no abstracts in English
Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
no journal, ,
NFujimaki, Shu; Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Ishioka, Noriko
no journal, ,
The positron-emitting tracer imaging system (PETIS) is one of the non-invasive imaging tools for living organisms. PETIS is specialized for plant nutrition with very compact detection heads which can be installed in a growth chamber. We have been developing the total methods of PETIS, including production of various positron-emitting radiotracers such as 
Cd, and many studies in plant nutrition have been done using PETIS in this decade. Here, we employed N for the study of symbiotic nitrogen fixation and successfully developed a rapid method to produce and purify N-labeled nitrogen gas. The gaseous tracer was fed as a mixture of N, O and He (10:20:70) to the underground part of intact nodulated soybean plants. As a result, serial images of distribution of the fixed N were obtained, and the fixation rates were estimated 0.17
0.10 
mol N h
plant (mean SD, n=6) from the data.
N-labeled nitrogen gasIshii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
no journal, ,
no abstracts in English
Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu
no journal, ,
no abstracts in English
