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USDA110 generated by ion-beam irradiationTakeda, Kiyoko*; Sato, Katsuya; Narumi, Issey*; Ono, Yutaka; Otsu, Naoko*; Yokoyama, Tadashi*
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 120, 2015/03
USDA110 generated by ion-beam irradiationTakeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 114, 2014/03
Makino, Amane*; Sueyoshi, Kuni*; Suzuki, Yuji*; Kawachi, Naoki; Tawaraya, Keitaro*; Otsu, Naoko*; Nakanishi, Hiromi*; Fujiwara, Toru*
Nihon Dojo Hiryo Gaku Zasshi, 82(2), p.152 - 159, 2011/04
This paper is a proceeding of the fourth division (Plant nutrition) symposium theme of discussed in the annual conference of 2011 soil science and plant nutrition at Hokkaido University. N. Kawachi, an author who takes charge of third section, discuss nutritional physiology revealed by imaging analysis of element kinetics in plants. This section focuses on introducing a RI imaging system developed in Japan Atomic Energy Agency. In addition, it is expressed that the analytical research conducted on nutritional physiology in plants as revealed by imaging of element kinetics.
USDA110 generated by ion-beam microbial mutation-breeding technologyTakeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
no journal, ,
We have tried to generate high temperature tolerant mutants of USDA110 using ion beam breeding technology. Consequently, we obtained a mutant, M14, which can survive at 42
C for at least 7 days. The genome sequence data of M14 were accumulated using a GS Junior System. The data were then mapped to the reference sequence of strain USDA110 using a GS Reference Mapper software. About 99.5% of the reference sequence was covered with the M14 sequence. Genome comparison revealed a large-scale structural change in the circular M14 genome. Namely, a DNA region at the coordinates 4,029,225 
5,299,697 (1,270,473 bp) was inverted. In addition, about 20 mutations such as base substitutions, insertions and deletions were found in M14. The acquirement of high temperature tolerance in M14 could be attributed to a large-scale inversion and/or small-scale mutations.
USDA110 generated by ion-beam irradiationTakeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
no journal, ,
USDA110 generated by ion-beam irradiationTakeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
no journal, ,
With the aims of utilization as a biofertilizer inoculant and elucidation of high-temperature tolerance mechanism of soybean nodule bacteria, we had applied the ion beam breeding technology to a strain (
) USDA110. Consequently, we obtained a mutant named M14 which was able to maintain high viable cell numbers under high-temperature (42C) for at least 7 days. Genome comparison with USDA110 revealed that (1) 1.27 Mbp inversion mutagenesis and (2) 18 single base mutations were occurred in M14. This study was the first attempt to apply the ion beam breeding technology to a prokaryote and succeeded in proving the effectiveness of it.
USDA110 generated by Ion-beam breeding technologySato, Katsuya; Takeda, Kiyoko*; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
no journal, ,
no abstracts in English
USDA110 generated by ion-beam irradiationTakeda, Kiyoko*; Sato, Katsuya; Ono, Yutaka; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*
no journal, ,
no abstracts in English
