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Inoue, Rintaro*; Oda, Takashi*; Nakagawa, Hiroshi; Tominaga, Taiki*; Saio, Tomohide*; Kawakita, Yukinobu; Shimizu, Masahiro*; Okuda, Aya*; Morishima, Ken*; Sato, Nobuhiro*; et al.
Scientific Reports (Internet), 10, p.21678_1 - 21678_10, 2020/12
Times Cited Count：3 Percentile：13.48(Multidisciplinary Sciences)Incoherent quasielastic neutron scattering (iQENS) is a fascinating technique for investigating the internal dynamics of protein. However, both low flux of neutron beam and absence of analytical procedure for extracting the internal dynamics from iQENS profile have been obstacles for studying it under physiological condition (in solution). Thanks to the recent development of neutron source, spectrometer and computational technique, they enable us to decouple internal dynamics, translational and rotational diffusions from the iQENS profile. The internal dynamics of two proteins: globular domain protein (GDP) and intrinsically disordered protein (IDP) in solution were studied. It was found that the average relaxation rate of IDP was larger than that of GDP. Through the detailed analyses on their internal dynamics, it was revealed that the fraction of mobile H atoms in IDP was much higher than that in GDP. Interestingly, the fraction of mobile H atoms was closely related to the fraction of H atoms on highly solvent exposed surfaces. The iQENS study presented that the internal dynamics were governed by the highly solvent exposed amino acid residues depending upon protein molecular architectures.
Iwase, Hiroki*; Kawai, Risa*; Morishima, Ken*; Takata, Shinichi; Yoshimura, Tomokazu*; Shibayama, Mitsuhiro*
Journal of Colloid and Interface Science, 538, p.357 - 366, 2019/03
Times Cited Count：13 Percentile：42.15(Chemistry, Physical)Oda, Takashi; Inoue, Rintaro*; Morishima, Ken*; Oi, Rika*; Ishino, Yoshizumi*; Sato, Mamoru*; Sugiyama, Masaaki*
no journal, ,
no abstracts in English
Oda, Takashi; Inoue, Rintaro*; Morishima, Ken*; Aizawa, Naoki*; Oi, Rika*; Ishino, Yoshizumi*; Oku, Takayuki; Sato, Mamoru*; Sugiyama, Masaaki*
no journal, ,
no abstracts in English
