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sp. nov., a mesophilic sulfate-reducing deltaproteobacterium isolated from a deep siliceous mudstone formationUeno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Tamaki, Hideyuki*; Naganuma, Takeshi*; et al.
International Journal of Systematic and Evolutionary Microbiology, 71(2), p.004683_1 - 004683_10, 2021/02
Times Cited Count：8 Percentile：62.07(Microbiology)A novel mesophilic sulfate-reducing bacterium, designated strain HN2
, was isolated from groundwater sampled from the subsurface Miocene Wakkanai Formation located in Horonobe, Hokkaido, Japan. The cells were Gram-negative rods, with motility conferred by a single polar flagellum. The isolate expressed desulfoviridin, but no catalase or oxidase activities was detected. Strain HN2 grew in a temperature range of 5-43 
C (optimum, 35 C) and in a pH range of 6.5-7.5 (optimum, pH 7.0-7.3). It used sulfate, thiosulfate, dimethyl sulfoxide, anthraquinone-2,6-disulfonate, Fe
, and manganese oxide as electron acceptors, but not elemental sulfur, nitrite, or nitrate. The bacterium showed very weak growth with sulfite as the electron acceptor. The strain fermented pyruvate and cysteine in the absence of sulfate, but not malate or succinate. The bacterium did not require NaCl, but tolerated up to 4% NaCl (w/v). Strain HN2 did not require vitamins. The G+C content of the genomic DNA was 56.66 mol%. A 16S rRNA gene sequence analysis showed that the closest recognized relative of strain HN2 is 
JS1 (97.0% similarity). The average nucleotide identity (ANI) value between strain HN2 and D. 
JS1 was 79.8%. Based on the phenotypic and molecular genetic evidence, the isolate is assigned to the new species sp. nov. The type strain is HN2 (=DSM 101010 =NBRC 112213).
Nakayama, Masashi; Saiga, Atsushi; Kimura, Shun; Mochizuki, Akihito; Aoyagi, Kazuhei; Ono, Hirokazu; Miyakawa, Kazuya; Takeda, Masaki; Hayano, Akira; Matsuoka, Toshiyuki; et al.
JAEA-Research 2019-013, 276 Pages, 2020/03
JAEA-Research-2019-013.pdf:18.72MB
The Horonobe Underground Research Laboratory (URL) Project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies for geological disposal of High-level Radioactive Waste through investigations of the deep geological environment within the host sedimentary rock at Horonobe Town in Hokkaido, north Japan. The investigations will be conducted in three phases, namely "Phase 1: Surface based investigations", "Phase 2: Construction phase" (investigations during construction of the underground facilities) and "Phase 3: Operation phase" (research in the underground facilities). According to the research plan described in the 3rd Mid- and Long- term Plan of JAEA, "Near-field performance study", "Demonstration of repository design option", and "Verification of crustal-movement buffering capacity of sedimentary rocks" are important issues of the Horonobe URL Project, and schedule of future research and backfill plans of the project will be decided by the end of 2019 Fiscal Year. The present report summarizes the research and development activities of these 3 important issues carried out during 3rd Medium to Long-term Research Phase.
Tanaka, Masaru*; Kawara, Osami*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Kawase, Keiichi; Tokizawa, Takayuki; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2018-001, 98 Pages, 2018/06
JAEA-Research-2018-001.pdf:2.49MB
In the 2016 fiscal year, communication cases on general waste disposal facility construction plans in recent years were surveyed. Results suggested as follows: (1) Existing long-term relationships or agreements in local area promote local accepting. (2) An operator needs to consider alternative plans and explain reasons for the decision making to local stakeholders. (3) Even after first announcement of a new plan, an operator needs to review the plan depending on local concerns. (4) Announcement of a new plan will activate communications on local development including the site redevelopment.
Sato, Toshinori; Aoyagi, Kazuhei; Matsuzaki, Yoshiteru; Miyara, Nobukatsu; Miyakawa, Kazuya
Rock Dynamics; Experiments, Theories and Applications, p.575 - 580, 2018/06
Rock dynamics is one of key issue for research and development of techniques for safe geological disposal of high-level radioactive waste. Horonobe Underground Research Laboratory (URL) is off-site URL constructed in soft sedimentary rock to the depth of 350m with three shafts and three level experimental galleries. Earthquake-resistant design of underground openings, observation of seismic records and groundwater pressure change due to earthquakes, and excavation disturbed zone experiment have been performed relating to the study of rock dynamics in URL project. This paper shows current status of Horonobe URL project and results of earthquake-resistant design of shafts, observation of seismic records and groundwater pressure change due to the 2011 off the Pacific coast of Tohoku Earthquake.
Tamamura, Shuji*; Miyakawa, Kazuya; Aramaki, Noritaka*; Igarashi, Toshifumi*; Kaneko, Katsuhiko*
Groundwater, 56(1), p.118 - 130, 2018/01
Groundwater saturated with gas forms bubble under atmospheric pressure, bothering most authorized methods to determine in-situ dissolved gas concentrations. To circumvent this problem, a simple theory to quantify effect of 
[bar]) of a gas component "
" (e.g., CH
, CO
and H) in equilibrium with in-situ dissolved concentration (
[mol L 
]) was related to partial pressure of the 
[bar]) emerged from groundwater in the form: 
= 
+ 
(k' /(
k' 
)), where [bar] is groundwater pressure difference before and after the 
" The k
' and k
' corresponds to the Henry's constant [bar L mol] of (k) and (k), respectively, except for k
', which is pH-dependent function. Dissolved CH concentrations were successfully estimated by the model within the error of 
4.0% from the direct measurements by the sealed sampler method. Similar TIC concentration in the groundwater before and after the rm bubble formation was consistent with the model prediction. The wide application of the model is suggested without selecting sampling locations.
Tanaka, Masaru*; Aoyama, Isao*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Kawase, Keiichi; Watanabe, Masanori; Tokizawa, Takayuki; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2017-003, 65 Pages, 2017/06
JAEA-Research-2017-003.pdf:2.92MB
JAEA Ningyo-toge Environmental Engineering Center and Fukushima Environmental Safety Center have same challenges in risk communication. As reference, similar domestic cases were investigated by our two Centers, and requirements for building long-term relationship were clarified. As follows; (1) Develop new relationship with various stakeholders in the region. (2) Make better use of existing resources (personnel, land and facilities, etc.). (3) Make a concerted effort to create new values with local stakeholders. (4) Make an opportunity which local stakeholders confirm safety and build confidence to the project. These efforts will enhance the opportunities for operators and residents to learn about environment management and environmental protection.
Tanaka, Masaru*; Aoyama, Isao*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2016-017, 76 Pages, 2017/01
JAEA-Research-2016-017.pdf:10.57MB
From 1955 to 2001, Japan Atomic Energy Agency (JAEA) carried out research and development projects related to uranium exploration, mining refining, conversion and enrichment at/around Ningyo-toge in Japan. Subsequently, JAEA has been conducting projects related to decommissioning of nuclear fuel cycle facilities and remediation of closed mine sites. JAEA had opportunities of communication with local stakeholders through the projects. Consensus building with local stakeholders and maintain it for over decades are important challenges for JAEA Ningyo-toge Environmental Engineering Center. For this aim, similar domestic case were investigated and compared, and evaluated about required measures for long term relationship with local stakeholder.
Sasano, Nakashi*; Enomoto, Atsushi*; Hosoi, Yoshio*; Katsumura, Yosuke; Matsumoto, Yoshihisa*; Shiraishi, Kenshiro*; Miyagawa, Kiyoshi*; Igaki, Hiroshi*; Nakagawa, Keiichi*
Journal of Radiation Research, 48(6), p.495 - 503, 2007/11
Times Cited Count：21 Percentile：53.82(Biology)Iwadachi, Takaharu*; Uchida, Munenori*; Mishima, Yoshinao*; Fujita, Akitsugu*; Kawamura, Hiroshi; Shestakov, V.*; Miyakawa, Masaru*
JAERI-Conf 2004-006, p.196 - 202, 2004/03
no abstracts in English
Miyakawa, Kazuya; Miyara, Nobukatsu; Tokuyasu, Kayoko; Shimizu, Mayuko
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Murakami, Takuma*; Miyakawa, Kazuya; Tamamura, Shuji*; Kiyama, Tamotsu*; Inomata, Hidenori*; Naganuma, Takeshi*; Kaneko, Katsuhiko*; Igarashi, Toshifumi*
no journal, ,
no abstracts in English
