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Journal Articles

Deep microbial life in high-quality granitic groundwater from geochemically and geographically distinct underground boreholes

Ino, Kohei*; Konno, Yuta*; Kozuka, Mariko*; Hirota, Akinari*; Togo, Yoko*; Fukuda, Akari*; Komatsu, Daisuke*; Tsunogai, Urumu*; Tanabe, Akifumi*; Yamamoto, Satoshi*; et al.

Environmental Microbiology Reports (Internet), 8(2), p.285 - 294, 2016/04

 Times Cited Count:26 Percentile:68.59(Environmental Sciences)

To understand the subsurface biosphere, borehole investigation was conducted for 300-m deep granitic rocks at the Mizunami underground research laboratory, Japan. The initial biomass was the highest with the flourish of aerobic H$$_{2}$$-oxidizing Hydrogenophaga spp., whereas an uncultivated lineage of the phylum Nitrospirae became predominant after three years with decreasing biomass. The common occurrence of many species of Nitrospirae and Chlorobi phyla at the geographically distinct sites and the exclusive detection of their phylogenetically related environmental sequences from deep groundwaters and terrestrial hot springs, suggest that these bacteria are indigenous and potentially adapted to the deep terrestrial subsurface.

Journal Articles

Biogeochemical signals from deep microbial life in terrestrial crust

Suzuki, Yohei*; Konno, Yuta*; Fukuda, Akari*; Komatsu, Daisuke*; Hirota, Akinari*; Watanabe, Katsuaki*; Togo, Yoko*; Morikawa, Noritoshi*; Hagiwara, Hiroki; Aosai, Daisuke*; et al.

PLOS ONE (Internet), 9(12), p.e113063_1 - e113063_20, 2014/12

 Times Cited Count:12 Percentile:34.33(Multidisciplinary Sciences)

We present multi-isotopic evidence of microbially mediated sulfate reduction in a granitic aquifer, a representative of the terrestrial crust habitat. Deep groundwater of meteoric origin was collected from underground boreholes drilled into the Cretaceous Toki granite, central Japan. A large sulfur isotopic fractionation of 20-60 permil diagnostic to microbial sulfate reduction is associated with the investigated groundwater containing sulfate below 0.2 mM. In contrast, a small carbon isotopic fractionation ($$<$$ 30 permil) is not indicative of methanogenesis. Our results demonstrate that the deep biosphere in the terrestrial crust is metabolically active and playing a crucial role in the formation of reducing groundwater even under low energy fluxes.

Journal Articles

Occurrence and potential activity of denitrifiers and methanogens in groundwater at 140 m depth in Pliocene diatomaceous mudstone of northern Japan

Katsuyama, Chie*; Nashimoto, Hiroaki*; Nagaosa, Kazuyo*; Ishibashi, Tomotaka*; Furuta, Kazuki*; Kinoshita, Takeshi*; Yoshikawa, Hideki; Aoki, Kazuhiro; Asano, Takahiro*; Sasaki, Yoshito; et al.

FEMS Microbiology Ecology, 86(3), p.532 - 543, 2013/12

 Times Cited Count:14 Percentile:36.72(Microbiology)

Anaerobic microbial activity has a major influence on the subsurface environment, and should be considered in subsurface activities including the construction of radioactive waste repositories. We investigated denitrification and methanogenesis in anoxic groundwater from 140 m depth in two boreholes, where the redox potential fluctuated. The average maximum potential denitrification rates, measured under anaerobic conditions in the two boreholes using an $$^{15}$$N tracer. Methanogenesis candidates were detected by 16S rRNA gene analysis. Although the stable isotope signatures suggested that some of the dissolved methane was of biogenic origin, no potential for methane production was evident during the incubations. The groundwater at 140 m depth did not contain oxygen, had an Eh ranging from -144 to 6.8 mV, and was found to be a potential field for denitrification.

Journal Articles

Stoichiometric study of the dielectric and magnetic properties in charge frustrated system LuFe$$_{2}$$O$$_{4}$$

Michiuchi, Takamasa*; Yokota, Yusuke*; Komatsu, Takuma*; Hayakawa, Hironori*; Kuroda, Tomoko*; Maeda, Daisuke*; Matsuo, Yoji*; Mori, Shigeo*; Yoshii, Kenji; Hanasaki, Noriaki*; et al.

Ferroelectrics, 378(1), p.175 - 180, 2009/00

 Times Cited Count:18 Percentile:59.66(Materials Science, Multidisciplinary)

We have synthesized the samples of LuFe$$_{2}$$O$$_{4}$$, which shows the ferroelectricity due to charge ordering of Fe ions, under several different reducing conditions using a CO$$_{2}$$-CO flow. The reducing condition was changed by changing a flow ratio of CO$$_{2}$$ and CO. It was found that the flow ratio of CO$$_{2}$$/CO of about 5 provided the sample with the highest magnetic transition temperature of 240-250 K. This sample showed the dielectric constant of 10000 at room temperature. The imaginary part of the dielectric response offered the activation energy of 0.4-0.5 eV, which is slightly larger than that reported previously (about 0.3 eV). We will perform further investigation of the physical properties of this sample to clarify full details and their origin of LuFe$$_{2}$$O$$_{4}$$.

Journal Articles

Magnetic and dielectric properties of HoFe$$_{2}$$O$$_{4}$$ and R$$_{1-x}$$R'$$_{x}$$Fe$$_{2}$$O$$_{4}$$ (R, R': rare earths)

Yoshii, Kenji; Yoneda, Yasuhiro; Maeda, Daisuke*; Yokota, Yusuke*; Michiuchi, Takamasa*; Komatsu, Takuma*; Ikeda, Naoshi*; Matsuo, Yoji*; Mori, Shigeo*

Japanese Journal of Applied Physics, 47(9), p.7599 - 7602, 2008/09

 Times Cited Count:8 Percentile:33.28(Physics, Applied)

We have investigated the physical properties of HoFe$$_{2}$$O$$_{4}$$ and R$$_{1-x}$$R'$$_{x}$$Fe$$_{2}$$O$$_{4}$$ (R, R': rare earths). Dielectric measurements showed their dielectric constants of 1000 at around room temperature, which is characteristic of the RFe$$_{2}$$O$$_{4}$$ series (R: rare earths). However, the magnetic transition temperatures and the coherency in dielectric response were found to be different from those of RFe$$_{2}$$O$$_{4}$$ reported so far. Interestingly, these materials suggested higher ferroelectric temperatures than those reported so far, suggesting a possibility of application of these materials.

Oral presentation

Hydrogen and carbon isotope geochemistry of freshwater aquifers at the Mizunami Underground Research Laboratory; Implications for ongoing biogeochemical processes in granitic rocks

Konno, Yuta*; Fukuda, Akari; Kozuka, Mariko*; Komatsu, Daisuke*; Tsunogai, Urumu*; Aosai, Daisuke; Mizuno, Takashi; Suzuki, Yohei*

no journal, , 

Our knowledge of biogeochemical processes mediated in those associated with freshwater should be integrated. We collected the groundwater samples from 200 m to 1150 m depths below ground level at Mizunami Underground Research Laboratory located in central Japan. We measured the concentration and both hydrogen and carbon isotopic compositions of dissolved methane, total inorganic carbon (TIC) and molecular hydrogen. The origin of methane could be speculated to be not biogenic CO$$_{2}$$ reduction or acetate fermentation but thermogenic or abiogenic. In contrast, C1/C2+C3 ratios supported biogenic methane production. The 300 m deep samples were enriched in hydrogen and acetate and depleted in sulfate, which is characteristic of acetogenesis. From these results, it is suggested that the production of acetate rather than methane could be dominant biogeochemical processes in the reducing portions of freshwater aquifers in granitic rocks.

Oral presentation

Shifts in microbial sulfate reduction to CO$$_{2}$$ reduction depending on groundwater flow characteristics in deep granitic aquifers

Konno, Yuta*; Komatsu, Daisuke*; Nishimura, Shusaku*; Fukuda, Akari; Aosai, Daisuke; Mizuno, Takashi; Nagao, Seiya*; Tsunogai, Urumu*; Suzuki, Yohei*

no journal, , 

no abstracts in English

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