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Journal Articles

The Role of ${it Deinococcus radiodurans}$ RecFOR proteins in homologous recombination

Sato, Katsuya; Kikuchi, Masahiro; Ishaque, A. M.*; Oba, Hirofumi*; Yamada, Mitsugu; Tejima, Kohei; Onodera, Takefumi; Narumi, Issei

DNA Repair, 11(4), p.410 - 418, 2012/04

https://doi.org/10.1016/j.dnarep.2012.01.008
 Times Cited Count：24 Percentile：59.83(Genetics & Heredity)

In an effort to gain insights into the role of ${it D. radiodurans}$ RecFOR proteins in homologous recombination, we generated ${it recF}$, ${it recO}$ and ${it recR}$ disruptant strains and characterized the disruption effects. Disruption of ${it recR}$ resulted in severe reduction of the transformation efficiency. On the other hand, the ${it recF}$ disruptant strain was the most sensitive phenotype to $$gamma$$ rays, UV irradiation and mitomycin C among the three disruptants. In the ${it recF}$ disruptant strain, the intracellular level of the LexA1 protein did not decrease following $$gamma$$ irradiation. These results demonstrate that the RecF protein plays a crucial role in the homologous recombination repair process by facilitating RecA activation. Thus, the RecF and RecR proteins are involved in the RecA activation and the stability of incoming DNA, respectively, during RecA-mediated homologous recombination processes that initiated the ESDSA pathway in ${it D. radiodurans}$.

Journal Articles

Development of a Rhenium-186-labeled MAG3-conjugated bisphosphonate for the palliation of metastatic bone pain based on the concept of bifunctional radiopharmaceuticals

Ogawa, Kazuma*; Mukai, Takahiro*; Arano, Yasushi*; Ono, Masahiro*; Hanaoka, Hirofumi*; Ishino, Seigo*; Hashimoto, Kazuyuki; Nishimura, Hiroshi*; Saji, Hideo*

Bioconjugate Chemistry, 16(4), p.751 - 757, 2005/07

https://doi.org/10.1021/bc040249w
 Times Cited Count：62 Percentile：86.86(Biochemical Research Methods)

Rhenium-186-1-hydroxyethylidene-1,1-diphosphonate ($$^{186}$$Re-HEDP) has been used for the palliation of metastatic bone pain. Delayed blood clearance and high gastric uptake of radioactivity have been observed upon injection, due to the instability of $$^{186}$$Re-HEDP in vivo. In this study, on the basis of the concept of bifunctional radiopharmaceuticals, we designed a stable $$^{186}$$Re-mercaptoacetylglycylglycylglycine (MAG3) complex-conjugated bisphosphonate ($$^{186}$$Re-MAG3-HBP). After purification by HPLC, $$^{186}$$Re-MAG3-HBP was synthesized with a radiochemical purity of over 95%. In biodistribution experiments, the radioactivity level of$$^{186}$$Re-MAG3-HBP in bone was significantly higher than that of $$^{186}$$Re-HEDP. Blood clearance of $$^{186}$$Re-MAG3-HBP was faster than that of $$^{186}$$Re-HEDP. In addition, the gastric accumulation of $$^{186}$$Re-MAG3-HBP radioactivity was lower than that of $$^{186}$$Re-HEDP. In conclusion, $$^{186}$$Re-MAG3-HBP is expected to be a useful radiopharmaceutical for the palliation of metastatic bone pain.

Journal Articles

Synthesis and evaluation of bisphosphonate derivative labeled with Rhenium-186 using monoaminemonoamidedithiols as a chelating group

Mukai, Takahiro*; Ogawa, Kazuma*; Arano, Yasushi*; Ono, Masahiro*; Fujioka, Yasushi*; Izumo, Mishiroku; Konishi, Junji*; Saji, Hideo*

Journal of Labelled Compounds and Radiopharmaceuticals, 44(Suppl.1), p.S617 - S618, 2001/05

no abstracts in English

Journal Articles

Labeling with radioactive rhenium for the purpose of the pain relief of cancerous bone metastasis

Ogawa, Kazuma*; Ono, Masahiro*; Fujioka, Yasushi*; Saji, Hideo*; Mukai, Takahiro*; Konishi, Junji*; Uehara, Tomoya*; Arano, Yasushi*; Onoma, Katsuyuki

Kaku Igaku, 37(5), P. 577, 2000/09

no abstracts in English

JAEA Reports

None

*; *

JNC TJ8420 2000-010, 171 Pages, 2000/03

JNC-TJ8420-2000-010.pdf:5.34MB

no abstracts in English

Journal Articles

Interaction between RF and edge plasma during ICRF heating in JT-60

Fujii, Tsuneyuki; Saigusa, Mikio; Kimura, Haruyuki; *; Tobita, Kenji; Nemoto, Masahiro; Kusama, Yoshinori; Seki, Masami; Moriyama, Shinichi; Nishitani, Takeo; et al.

Fusion Engineering and Design, 12, p.139 - 148, 1990/00

https://doi.org/10.1016/0920-3796(90)90074-G
 Times Cited Count：25 Percentile：89.09(Nuclear Science & Technology)

no abstracts in English

JAEA Reports

Fuel Centerline Temperature Measurement Experiment in JMTR(III) -Post-Irradiation Examination of the First Test Assembly -

; ; ; ; ; ; ; ; ; ; et al.

JAERI-M 82-034, 55 Pages, 1982/04

JAERI-M-82-034.pdf:4.63MB

no abstracts in English

Oral presentation

Synthesis and evaluation of $$^{186}$$Re-labeled biotin as a labeling agent for antibodies

Hirasawa, Makoto*; Kawashima, Hidekazu*; Ogawa, Kazuma*; Kimura, Hiroyuki*; Ono, Masahiro*; Hashimoto, Kazuyuki; Saji, Hideo*

no journal, , 

no abstracts in English

Oral presentation

Importance of RecFOR in homologous recombination mechanism in ${it Deinococcus radiodurans}$

Sato, Katsuya; Kikuchi, Masahiro; Oba, Hirofumi*; Yamada, Mitsugu; Tejima, Kohei; Onodera, Takefumi; Narumi, Issei

no journal, , 

In an effort to gain an insight into the role of the RecFOR in DNA repair through homologous recombination in ${it Deinococcus radiodurans}$, we generated a RecFOR disruptant strains and characterized the disruption effects. The RecF disruptant strain exhibited sensitivity to DNA-damaging agents, suggesting that RecF has a crucial role in DNA repair. This result indicates that RecF takes an important role in the RecA activation in the homologous recombination process with DNA damage. While, the recombination assay revealed that RecR is essential for the process of homologous intermolecular recombination, suggesting that RecR is important factor in the homologous recombination process without DNA damage. The importance of RecF and RecR in the homologous recombination processes is reversed due to the existence of DNA damage. Therefore, ${it D. radiodurans}$ will use the different homologous recombination processes through the RecFOR or RecOR, respectively.

Oral presentation

Pretargeted radioimmunotherapy of tumor using a novel radiorhenium-labeled biotin derivative and streptavidin conjugated anti-cytokeratin 19 monoclonal antibody

Kawashima, Hidekazu*; Hirasawa, Makoto*; Kimura, Hiroyuki*; Ono, Masahiro*; Hashimoto, Kazuyuki; Saji, Hideo*

no journal, , 

no abstracts in English

10 (Records 1-10 displayed on this page)
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