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resolutionYagi, Daichi*; Yamada, Taro*; Kurihara, Kazuo; Onishi, Yuki*; Yamashita, Masahiro*; Tamada, Taro; Tanaka, Ichiro*; Kuroki, Ryota; Niimura, Nobuo*
Acta Crystallographica Section D, 65(9), p.892 - 899, 2009/09
Times Cited Count：17 Percentile：80.39(Biochemical Research Methods)A neutron crystallographic analysis of phosphate-free bovine pancreatic RNase A has been carried out at 1.7 resolution using the BIX-4 single-crystal diffractometer at the JRR-3 reactor of the Japan Atomic Energy Agency. The high resolution structural model allowed us to determine that His12 acts mainly as a general base in the catalytic process of RNase A. Numerous other distinctive structural features such as the hydrogen positions of methyl groups, hydroxyl groups, prolines, asparagines and glutamines were also determined at 1.7 resolution. The protonation and deprotonation states of all of the charged amino-acid residues allowed us to provide a definitive description of the hydrogen-bonding network around the active site and the H atoms of the key His48 residue. Differences in hydrogen-bond strengths for the 
-helices and 
-sheets were inferred from determination of the hydrogen-bond lengths and the H/D-exchange ratios of the backbone amide H atoms. The correlation between the B factors and hydrogen-bond lengths of the hydration water molecules was also determined.
10th International Congress of the International Radiation Protection Association, 0 Pages, 1999/00
None
Fuchiwaki, Hirotaka; Munakata, Masahiro; Kimura, Hideo; Imai, Hisashi*; Shiozaki, Isao*; Yamashita, Ryo*
no journal, ,
It is thought that in an external factor that influences the underground flow, there is a climatic variation repeated at the cycle of the order of 100,000 years, the following phenomena are caused by this climatic variation, and it influences the underground flow. It is thought that the following phenomena are caused by this climatic variation, and it influences the underground flow. (1) Development and disappearance of glacier, (2) Change in precipitation, (3) Decrease in amount of training because of development of frozen soil, (4) Sea-level rhythms, (5) Upheaval and erosion by gaining the weight to the earth's crust according to development of river and disappearance, (6) Dilution of seawater according to glacier disappearance, etc. The analytical result and the evaluation are announced.
Kato, Shingo; Yoshida, Keisuke; Inoue, Mutsuo*; Yamashita, Masahiro; Okuyama, Shinichi; Ishimori, Yuu
no journal, ,
no abstracts in English
Be depositions in Hokuriku, JapanYoshida, Keisuke; Kato, Shingo; Uchida, Kengo*; Yamashita, Masahiro; Okuyama, Shinichi; Inoue, Mutsuo*
no journal, ,
In winter, Be deposition profile shows wide variation in Hokuriku region. Temporal and regional variations of Be deposition data during 30 years (Apr. 1991 to Mar. 2021) were analyzed and material transport mechanisms from arctic region were studied. Due to pressure pattern around Japan in winter and inhomogeneous precipitation in Hokuriku region caused by topography, characteristic seasonal variation was found and highest Be deposition was observed in mountainous area in Kanazawa City. In addition, the period of high Be deposition observed approximately agreed with that of minimum sunspot number or maximum precipitation. Therefore, sunspot number and precipitation affect temporal variations of Be deposition.
Hagiwara, Hiroki; Ishimori, Yuu; Yoshida, Keisuke; Nakano, Masanao; Tsunokawa, Shoji; Yamashita, Masahiro
no journal, ,
no abstracts in English
Be and 
Na deposition in the Hokuriku region in winterYoshida, Keisuke; Tsunokawa, Shoji; Umeda, Masayuki; Yamashita, Masahiro; Nakano, Masanao; Ishimori, Yuu; Inoue, Mutsuo*
no journal, ,
no abstracts in English
