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Ishino, Masahiko; Kado, Masataka; Shinohara, Kunio*; Yamamoto, Yoshimasa*; Hirai, Itaru*; Kishimoto, Maki; Nishikino, Masaharu; Hasegawa, Noboru; Tamotsu, Satoshi*; Yasuda, Keiko*; et al.
Proceedings of SPIE Europe Optics + Optoelectronics 2011, Vol.8139, p.81390R_1 - 81390R_8, 2011/09
Times Cited Count：0 Percentile：0.01(Optics)Ultra thin gold films are favorable laser plasma targets for a soft X-ray microscopy, because the thin films emit intense soft X-rays at the wavelength of water window region. Using rear side emissions, the distance between the X-ray source and the specimens can be reduced so that the X-ray flux on specimens increases. The microscope system can be designed to be compact when the specimen holder and X-ray source are combined in one piece. The biological specimen holder combined with an ultra thin film target has been developed. This X-ray microscope system needs not any X-ray optics which causes a decrease in X-ray photons for imaging. X-ray images of hydrated living cells have been obtained successfully by use of the newly developed specimen holder. Specimen holder combined with plasma X-ray source will be a key component of a compact soft X-ray microscope using in a laboratory.
Ishino, Masahiko; Kado, Masataka; Kishimoto, Maki; Nishikino, Masaharu; Hasegawa, Noboru; Oba, Toshiyuki; Kawachi, Tetsuya; Tamotsu, Satoshi*; Yasuda, Keiko*; Yamamoto, Yoshimasa*; et al.
no journal, ,
no abstracts in English
Kado, Masataka; Ishino, Masahiko; Kishimoto, Maki; Shinohara, Kunio*; Tamotsu, Satoshi*; Yasuda, Keiko*; Yamamoto, Yoshimasa*; Kinjo, Yasuhito*
no journal, ,
Contact soft X-ray microscopes with laser plasma X-ray sources are anticipated technology to be able to observe live hydrated biological cells with high spatial resolution. However there were no works reported which identified specific organelles clearly, because the obtained X-ray images are too complicated due to overlapping of cell structures. We have observed the same biological cells with both of a contact soft X-ray microscope and a confocal laser microscope and compared images obtained with both microscopes in order to identify organelles obtained with the soft X-ray microscope. As the results we succeeded to identify actin filaments and mitochondria clearly and found that the organelles obtained with the soft X-ray microscope were more detailed than those with the confocal laser microscope.
