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Journal Articles

Application of real time PCR for the quantitative detection of radiation-induced genomic DNA strand breaks

Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; et al.

Journal of Insect Biotechnology and Sericology, 77(1), p.17 - 24, 2008/02

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with $$^{137}$$Cs $$gamma$$-rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by $$gamma$$-ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.

Journal Articles

Natural circular dichroism of amino acid films observed in soft X-ray and VUV region using polarizing undulator

Nakagawa, Kazumichi*; Kaneko, Fusae*; Ota, Yoshimi*; Tanaka, Masahito*; Kitada, Tomo*; Agui, Akane; Fujii, Kentaro; Yokoya, Akinari; Watanabe, Kazutoshi*; Yamada, Toru*

Journal of Electron Spectroscopy and Related Phenomena, 144-147, p.271 - 273, 2005/06

 Times Cited Count:12 Percentile:50.31(Spectroscopy)

no abstracts in English

Journal Articles

Degradation of amino acids irradiated with vacuum-UV radiation(60nm, 120nm)

Yokoya, Akinari; Kobayashi, Katsumi*; Usami, Noriko*; *; Hieda, Kotaro*; *

Shinku Shigaisen (50nm Ijo) Niyoru Kakusan Sonsho Yuhatsu Kiko No Sogoteki Kenkyu, p.56 - 59, 1992/03

no abstracts in English

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