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Fukuda, Akari*; Hagiwara, Hiroki; Ishimura, Toyoho*; Kozuka, Mariko*; Ioka, Seiichiro*; Amano, Yuki; Tsunogai, Urumu*; Suzuki, Yohei*; Mizuno, Takashi
Microbial Ecology, 60(1), p.214 - 225, 2010/05
Times Cited Count：29 Percentile：65.76(Ecology)To better understand the geochemical and microbiological relationships, we characterized granitic groundwater collected from a 1,148 to 1,169 -m deep borehole interval at the Mizunami Underground Research Laboratory site, Japan, in 2005 and 2008. Geochemical analyses of the groundwater samples indicated that major electron acceptors, such as NO
and SO
, were not abundant, while dissolved organic carbon (not including organic acids), CH and H
were moderately rich. The most common phylotypes were both related to 
spp., the cultivated members of which can utilize minor electron donors, such as aromatic and aliphatic hydrocarbons. Geomicrobiological results suggest that deep granitic groundwater has been stably colonized by spp. probably owing to the limitation of O, NO and organic acids.
Fukuda, Akari*; Hagiwara, Hiroki; Ishimura, Toyoho*; Kozuka, Mariko*; Ito, Kazumasa*; Tsunogai, Urumu*; Suzuki, Yohei*; Mizuno, Takashi
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JAEA and AIST have been conducting collaborative work on hydrochemical study at MIU construction site. The aim of this collaborative study is to establish the methodology for investigation, analysis and evaluation of colloid/organics/microbes study. This paper reports the biogeochemical study. As the result, it is suggested that groundwater taken from deeper part (1150m depth) is relatively oxidized.
Suzuki, Yohei*; Fukuda, Akari*; Kozuka, Mariko*; Ishimura, Toyoho*; Tsunogai, Urumu*; Hagiwara, Hiroki; Mizuno, Takashi
no journal, ,
no abstracts in English
Ito, Kazumasa*; Suzuki, Yohei*; Fukuda, Akari*; Kozuka, Mariko*; Mizuno, Takashi; Hagiwara, Hiroki
no journal, ,
no abstracts in English
Fukuda, Akari; Kozuka, Mariko*; Aosai, Daisuke; Hagiwara, Hiroki; Mizuno, Takashi; Suzuki, Yohei*
no journal, ,
no abstracts in English
Konno, Yuta*; Fukuda, Akari; Kozuka, Mariko*; Komatsu, Daisuke*; Tsunogai, Urumu*; Aosai, Daisuke; Mizuno, Takashi; Suzuki, Yohei*
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Our knowledge of biogeochemical processes mediated in those associated with freshwater should be integrated. We collected the groundwater samples from 200 m to 1150 m depths below ground level at Mizunami Underground Research Laboratory located in central Japan. We measured the concentration and both hydrogen and carbon isotopic compositions of dissolved methane, total inorganic carbon (TIC) and molecular hydrogen. The origin of methane could be speculated to be not biogenic CO reduction or acetate fermentation but thermogenic or abiogenic. In contrast, C1/C2+C3 ratios supported biogenic methane production. The 300 m deep samples were enriched in hydrogen and acetate and depleted in sulfate, which is characteristic of acetogenesis. From these results, it is suggested that the production of acetate rather than methane could be dominant biogeochemical processes in the reducing portions of freshwater aquifers in granitic rocks.
Fukuda, Akari; Mizuno, Takashi; Aosai, Daisuke; Hagiwara, Hiroki; Yamamoto, Yuhei; Shingu, Shinya; Ito, Kazumasa*; Suzuki, Yohei*; Kozuka, Mariko*; Konno, Yuta*
no journal, ,
no abstracts in English
Fukuda, Akari; Kozuka, Mariko*; Konno, Yuta*; Aosai, Daisuke; Hagiwara, Hiroki; Mizuno, Takashi; Suzuki, Yohei*
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To quantify microbial influences on the hydrogeochemistry of a 99-1169-m deep granitic aquifer system, we developed methodology for sensitive measurements of a variety of aerobic and anaerobic metabolic activities. Briefly, microbial cells were ca. 30-fold concentrated in groundwater by filtration to incubate with electron acceptors. Aerobic respiration was more than 400 umol/L/year at depths of 99 and 175 m and decreased with increasing depth down to 36 umol/L/year at a depth of 1169 m. Nitrate respiration increased from 99 to 308 m (4.3 to 37 umol/L/year) and decreased with increasing depth down to 0.20 umol/L/year. Sulfate respiration ranging from 1.4 to 3.2 umol/L/year was detected only at depths above 200 m. The depletion of O and NO and a gradual decrease in sulfate with increasing depth in the granitic aquifer could be attributed to microbial respirations at rates clarified in this study. Our research was partially founded by Nuclear and Industry Safety Agency.
Fukuda, Akari; Mizuno, Takashi; Aosai, Daisuke; Hagiwara, Hiroki; Yamamoto, Yuhei; Shingu, Shinya; Takeno, Naoto*; Suzuki, Yohei*; Konno, Yuta*; Kozuka, Mariko*
no journal, ,
no abstracts in English
Fukuda, Akari; Kozuka, Mariko*; Konno, Yuta*; Aosai, Daisuke; Shingu, Shinya; Hagiwara, Hiroki; Mizuno, Takashi; Suzuki, Yohei*
no journal, ,
no abstracts in English
Suzuki, Yohei*; Fukuda, Akari; Konno, Yuta*; Kozuka, Mariko*; Hagiwara, Hiroki; Aosai, Daisuke; Takeno, Naoto*; Mizuno, Takashi
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