Phenotypic characterization of high temperature tolerant mutants of USDA110 generated by ion-beam irradiation

Takeda, Kiyoko; Tejima, Kohei*; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 107, 2013/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2012-046.pdf

The Effect of -sterilization of biofertilizer's carrier on bacterial inoculants survival

Tejima, Kohei*; Yokoyama, Tadashi*; Sato, Katsuya; Takeda, Kiyoko; Narumi, Issei

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 112, 2013/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2012-046.pdf

The Effect of -sterilization of carrier materials made with different types of soils on the shelf life of biofertilizer containing strain USDA110

Tejima, Kohei; Sato, Katsuya; Takeda, Kiyoko; Yokoyama, Tadashi*; Narumi, Issei

Radioisotopes, 61(4), p.161 - 171, 2012/04

https://doi.org/10.3769/radioisotopes.61.161

A biofertilizer is a substance that holds beneficial microorganisms for plant growth in a carrier material. To demonstrate the effect of -sterilization, the survival of the was monitored to assess the shelf life of biofertilizers. As biofertilizer carriers, five kinds of typical Japanese soil-based materials were used. Following the sterilization of carrier materials by -irradiation or autoclaving, was inoculated into each material. The biofertilizer was stored for 12 months at 4C or 30C. After storage, viable inoculants in the biofertilizer were enumerated. Results indicated that inoculant density after storage was greater than the initial density in biofertilizers made from sterilized carriers, whereas it decreased significantly in biofertilizers made from non-sterilized carriers. -sterilization was superior to autoclave sterilization in enhancing inoculant survival in some cases.

Colony morphology of high temperature tolerant mutants of USDA 110 obtained by ion-beam microbial mutation-breeding technology

Takeda, Kiyoko; Tejima, Kohei; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 110, 2012/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2011-043.pdf

Acquisition of new useful characteristics by microbial breeding using ion beams

Tejima, Kohei; Sato, Katsuya; Narumi, Issei

Tsuchi To Biseibutsu, 65(1), p.78 - 82, 2011/04

https://doi.org/10.18946/jssm.65.1_78

Along with world population growth, production increase and stable supply of food as well as global environment conservation are critical issues. Decrease in agricultural productivity and increase in food consumption in recent years are a grave situation in Japan because of Japan's low self-sufficiency in food. The spread of biological resources that possess environmental resistance and new functionality is necessary to make possible production increase and stable supply of food as well as global environment conservation. Since mutation breeding can contribute to spread biological resources, Quantum Beam Science Directorate, JAEA, has engaged in R&D on ion beam mutation breeding using the cyclotron at TIARA. This article presents an overview of the current state of plant mutation breeding technology using ion beam and its expansive application to microbial mutation breeding.

Phenotypic improvement of USDA 110 into a high temperature tolerant strain in terms of ion-beam microbial mutation-breeding technology

Takeda, Kiyoko*; Tejima, Kohei; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 75, 2011/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2010-065.pdf

The Effect of -sterilization of carrier materials on the shelf life of biofertilizer

Tejima, Kohei; Yokoyama, Tadashi*; Sato, Katsuya; Narumi, Issei

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 83, 2011/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2010-065.pdf

no abstracts in English

The Advantage of -irradiation of biofertilizer's carrier in bacterial inoculants survival

Tejima, Kohei; Yokoyama, Tadashi*; Sato, Katsuya; Narumi, Issei

no journal, , 

Biofertilizer is a substance that holds beneficial microorganism for plant growth (e.g. ) in the carrier such as peat, perlite, charcoal and soil. To use biofertilizer more efficiently, it is necessary to keep the amount of viable inoculants in the biofertilizer for a definite period of time. The inoculants survival in the biofertilizer could be affected by the physical and chemical properties of carrier materials and by the competition with native microorganisms in the carrier. -irradiation is expected to sterilize the carrier materials without changes in physical and chemical properties. In an effort to demonstrate the advantage of -sterilization, the survivals of inoculants were monitored to assess the shelf life of biofertilizer.

Importance of RecFOR in homologous recombination mechanism in

Sato, Katsuya; Kikuchi, Masahiro; Oba, Hirofumi*; Yamada, Mitsugu; Tejima, Kohei; Onodera, Takefumi; Narumi, Issei

no journal, , 

In an effort to gain an insight into the role of the RecFOR in DNA repair through homologous recombination in , we generated a RecFOR disruptant strains and characterized the disruption effects. The RecF disruptant strain exhibited sensitivity to DNA-damaging agents, suggesting that RecF has a crucial role in DNA repair. This result indicates that RecF takes an important role in the RecA activation in the homologous recombination process with DNA damage. While, the recombination assay revealed that RecR is essential for the process of homologous intermolecular recombination, suggesting that RecR is important factor in the homologous recombination process without DNA damage. The importance of RecF and RecR in the homologous recombination processes is reversed due to the existence of DNA damage. Therefore, will use the different homologous recombination processes through the RecFOR or RecOR, respectively.

Lethal effects of different LET radiations on the radioresistant bacterium

Sato, Katsuya; Tejima, Kohei; Narumi, Issei

no journal, , 

no abstracts in English