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Ueno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Tamaki, Hideyuki*; Naganuma, Takeshi*; et al.
International Journal of Systematic and Evolutionary Microbiology, 71(2), p.004683_1 - 004683_10, 2021/02
Times Cited Count:6 Percentile:63.69(Microbiology)A novel mesophilic sulfate-reducing bacterium, designated strain HN2, was isolated from groundwater sampled from the subsurface Miocene Wakkanai Formation located in Horonobe, Hokkaido, Japan. The cells were Gram-negative rods, with motility conferred by a single polar flagellum. The isolate expressed desulfoviridin, but no catalase or oxidase activities was detected. Strain HN2 grew in a temperature range of 5-43 C (optimum, 35 C) and in a pH range of 6.5-7.5 (optimum, pH 7.0-7.3). It used sulfate, thiosulfate, dimethyl sulfoxide, anthraquinone-2,6-disulfonate, Fe, and manganese oxide as electron acceptors, but not elemental sulfur, nitrite, or nitrate. The bacterium showed very weak growth with sulfite as the electron acceptor. The strain fermented pyruvate and cysteine in the absence of sulfate, but not malate or succinate. The bacterium did not require NaCl, but tolerated up to 4% NaCl (w/v). Strain HN2 did not require vitamins. The G+C content of the genomic DNA was 56.66 mol%. A 16S rRNA gene sequence analysis showed that the closest recognized relative of strain HN2 is JS1 (97.0% similarity). The average nucleotide identity (ANI) value between strain HN2 and D. JS1 was 79.8%. Based on the phenotypic and molecular genetic evidence, the isolate is assigned to the new species sp. nov. The type strain is HN2 (=DSM 101010 =NBRC 112213).
Miyazawa, Daisuke*; Sanada, Hiroyuki; Kiyama, Tamotsu*; Sugita, Yutaka; Ishijima, Yoji*
Journal of MMIJ, 127(3), p.132 - 138, 2011/03
The geology around the Japanese coast exhibits a wide distribution of mudstones composed of fossil diatoms and silica rocks. These bedrocks are categorized as porous and low permeability rock. When underground openings at rocks with such rock mechanics properties are constructed, evaluation which takes into account flow-stress coupling behavior is required. Poroelasticity treats both pore fluids and the mineral particles which make up rocks as compressible. Consequently, its application can extend to rock that is harder than soil materials. In this study, laboratory experiments by using the siliceous rocks distributed in the Horonobe area, Hokkaido, Japan were carried out as a case study. Proposal method for obtaining poroelasic coefficients as well as determination of poroelastic coefficients distributed in the Horonobe area are described in this paper.
Miyazawa, Daisuke*; Kiyama, Tamotsu*; Ishijima, Yoji*; Sugita, Yutaka; Sanada, Hiroyuki
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no abstracts in English
Miyazawa, Daisuke*; Kiyama, Tamotsu*; Ishijima, Yoji*; Kumakura, Satoshi*; Sugita, Yutaka; Sanada, Hiroyuki
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no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
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no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Murakami, Takuma*; Miyakawa, Kazuya; Tamamura, Shuji*; Kiyama, Tamotsu*; Inomata, Hidenori*; Naganuma, Takeshi*; Kaneko, Katsuhiko*; Igarashi, Toshifumi*
no journal, ,
no abstracts in English