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Wada, Seiichi*; Ando, Tatsuhiko*; Watanabe, Aya*; Kakizaki, Takehiko*; Natsuhori, Masahiro*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 79, 2015/03
So far, we clarified that X-ray irradiation induced cell killing by bystander effect mediated-secreted factor. This phenomenon was related with sphingomyelinase (SMase). In this study we analyzed mechanism of secreted SMase from irradiated cells after irradiation. SMase was detected in the culture medium after irradiation by SDS-PAGE. Then, SMase was detected in the exosome of culture medium, but not out of exosome after irradiation. This result indicates that SMase was secreted as exosome from the irradiated cells.
Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Shimozawa, Yoko; Fukamoto, Kana*; Yokota, Yuichiro; Sora, Sakura*; Kakizaki, Takehiko*; Wada, Seiichi*; Funayama, Tomoo; et al.
Biological Sciences in Space, 26, p.21 - 25, 2012/10
High linear energy transfer (LET) radiation is important cosmic rays that has neurobiological effects: it is known to induce conditioned taste aversion, and suppress neurogenesis that may underlie cognitive impairment. However, the impact of high-LET radiation on other learning effects remains largely unknown. Here, we focus on kinetics of the radiation response for the salt chemotaxis learning (SCL) behavior in the nameatode, , because the SCL during the learning conditioning was modulated after low-LET 
-irradiation. Firstly, the SCL ability was examined following high-LET irradiation (
C, 18.3 MeV/u, LET = 113 keV/
m), revealing its dose-dependent decrease after high- and low-LET exposure. Next, we demonstrate that the SCL at the early phase of the learning conditioning is greatly affected by high- and low-LET irradiation, and interestingly, the magnitude of these effects by high-LET radiation was smaller than that by low-LET one. Moreover, the analysis of 
mutant showed that the G-protein subunit, GPC-1 is responsible for such early phase response. This study is the first to provide the evidence for the kinetics of changes in SCL after high-LET irradiation of C. 
.
against high-LET radiation exposureSakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Shimozawa, Yoko; Fukamoto, Kana*; Yokota, Yuichiro; Sora, Sakura*; Kakizaki, Takehiko*; Wada, Seiichi*; Funayama, Tomoo; et al.
Biological Sciences in Space, 26, p.7 - 11, 2012/07
Here, we investigated the resistance to high-LET radiation exposure for two behaviors of the nematode, , which is known as a model organism for the nervous system. Tested behaviors were locomotion and chemotaxis to NaCl. In addition, egg hatchability was examined as an indicator of high-LET radiation sensitivity. Relative biological effectiveness (RBE) of high-LET radiation (C, 18.3 MeV/u, LET = 113 keV/m) relative to low-LET radiation for hatchability was 4.5, whereas RBEs for locomotion and chemotaxis were 1.4 and 1.1, respectively. This study shows that the behavioral system for locomotion and chemotaxis of 
is highly resistant to high-LET radiation exposure.
, induces the somatic mutationFurusawa, Toshiharu*; Fukamoto, Kana*; Sakashita, Tetsuya; Suzuki, Eiko*; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Funayama, Tomoo; Suzuki, Hiromi*; Ishioka, Noriaki*; Wada, Seiichi*; et al.
Journal of Radiation Research, 50(4), p.371 - 375, 2009/07
Times Cited Count：9 Percentile：32.08(Biology)Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, . On one hand, carbon-ion exposure of embryo to 0.5 - 6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.
Harada, Kosaku*; Nonaka, Tetsuo*; Hamada, Nobuyuki*; Sakurai, Hideyuki*; Hasegawa, Masatoshi*; Funayama, Tomoo; Kakizaki, Takehiko*; Kobayashi, Yasuhiko; Nakano, Takashi*
Cancer Science, 100(4), p.684 - 688, 2009/04
Times Cited Count：56 Percentile：75.67(Oncology)
Miyazawa, Yutaka*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; et al.
Journal of Radiation Research, 49(4), p.373 - 379, 2008/07
Times Cited Count：28 Percentile：62.78(Biology)We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporary suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed 
expression of 
gene, but not 
gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
Furusawa, Toshiharu*; Suzuki, Eiko*; Nagaoka, Shunji*; Suzuki, Hiromi*; Ishioka, Noriaki*; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko; Sakashita, Tetsuya; Kakizaki, Takehiko*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 115, 2008/03
Using heavy ion microbeam, we investigated the somatic mutation arising on the larvae in embyro and yolk in the egg of silkworm, . The incidence of the somatic mutation was 12%, and the same level of mutation following the microbeam irradiation at the center of the egg. However, the microbeam irradiation to the abdomen of the silkworm larvae induced the increase of somatic mutation, 63% (3 Gy) and 80% (6 Gy).
using heavy-ion microbeamMiyazawa, Yutaka*; Sakashita, Tetsuya; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; Funayama, Tomoo; Hamada, Nobuyuki*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 116, 2008/03
We examined the role of root cap and elongation zone cells in root hydrotropism of using heavy ion and laser microbeam. Heavy ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporarily suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy ion broad beam irradiation suppressed de novo expression of INDOLE ACETIC ACID 5 gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that de novo gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
-rays and heavy-ion beam in 
Bolige, A.*; Sakashita, Tetsuya; Kakizaki, Takehiko*; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko; Goto, Ken*
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 117, 2008/03
We report here how these rhythms are related to each other. First, irradiation temperatures influenced neither survival after exposure to UV, rays, nor C ions, whereas the incubation at high temperatures (25 
C) after irradiation considerably lowered the survival particularly after irradiation, but not after UV irradiation at all. Secondly, although the antioxidants 
carotene and dimethylsulfoxide (DMSO) increased the survival of the alga at the least resistant phase after UV C and UV B irradiation, respectively, to the level of the most resistant alga, none of these were not so effective for or C ion irradiation. Finally, FITC dextran was incorporated into the alga only after C ion irradiation but not irradiation, suggesting that C ion may perforate cell membranes to kill the alga.
to high-LET carbon ion beam irradiationSakashita, Tetsuya; Suzuki, Michiyo; Kakizaki, Takehiko*; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 111, 2008/03
We investigated the high LET carbon ions induced response of salt chamotaxis learning in . The performance of the salt chemotaxis learning was normal even after the exposure at the dose of 100 - 500 Gy. Also, the normal chemotaxis to benzaldehyde was normal, whereas the salt chemotaxis learning was affected by carbon irradiation. These results suggest the specific effect of carbon ions on the neuron network in .
Funayama, Tomoo; Wada, Seiichi*; Yokota, Yuichiro; Fukamoto, Kana; Sakashita, Tetsuya; Taguchi, Mitsumasa; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Suzuki, Michiyo; Furusawa, Yoshiya*; et al.
Journal of Radiation Research, 49(1), p.71 - 82, 2008/01
Times Cited Count：47 Percentile：78.7(Biology)Research concerning cellular responses to low dose irradiation, radiation-induced bystander effects, and the biological track structure of charged particles has recently received particular attention in the field of radiation biology. Target irradiation employing a microbeam represents a useful means of advancing this research by obviating some of the disadvantages associated with the conventional irradiation strategies. The heavy-ion microbeam system at JAEA-Takasaki can provide target irradiation of heavy charged particles to biological material at atmospheric pressure using a minimum beam size 5 m in diameter. The system can be applied to the investigation of mechanisms within biological organisms not only in the context of radiation biology, but also in the fields of general biology such as physiology, developmental biology and neurobiology, and should help to establish and contribute to the field of "microbeam biology".
Kakizaki, Takehiko; Hamada, Nobuyuki*; Sakashita, Tetsuya; Wada, Seiichi*; Hara, Takamitsu*; Funayama, Tomoo; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Kobayashi, Yasuhiko; et al.
Journal of Veterinary Medical Science, 69(6), p.605 - 609, 2007/06
Times Cited Count：1 Percentile：13.67(Veterinary Sciences)no abstracts in English
Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.
Journal of Radiation Research, 48(3), p.247 - 253, 2007/05
Times Cited Count：17 Percentile：47.8(Biology)To carry out the radio-microsurgery study using silkworm, , we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon (C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.
Kanasugi, Yuichi*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Sakashita, Tetsuya; Kakizaki, Takehiko; Kobayashi, Yasuhiko; Takakura, Kaoru*
International Journal of Radiation Biology, 83(2), p.73 - 80, 2007/02
Times Cited Count：31 Percentile：87.87(Biology)no abstracts in English
Kobayashi, Yasuhiko; Funayama, Tomoo; Sakashita, Tetsuya; Furusawa, Yoshiya*; Wada, Seiichi*; Yokota, Yuichiro; Kakizaki, Takehiko; Hamada, Nobuyuki*; Hara, Takamitsu*; Fukamoto, Kana; et al.
JAEA-Conf 2007-002, p.28 - 35, 2007/02
no abstracts in English
Funayama, Tomoo; Kakizaki, Takehiko; Wada, Seiichi*; Yokota, Yuichiro; Sakashita, Tetsuya; Hamada, Nobuyuki*; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 100, 2007/02
Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Sakashita, Tetsuya; Kakizaki, Takehiko; Ni, M.*; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 101, 2007/02
embryosTatei, Kazuaki*; Tamaki, Tomoaki*; Kawamura, Hidemasa*; Hamada, Nobuyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Kakizaki, Takehiko; Nonaka, Tetsuo*; Obinata, Hideru*; et al.
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 103, 2007/02
no abstracts in English
Yorifuji, Hiroshi*; Hino, Mizuki*; Wada, Seiichi*; Tajika, Yuki*; Morimura, Yoshihiro*; Hamada, Nobuyuki*; Funayama, Tomoo; Sakashita, Tetsuya; Kakizaki, Takehiko; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 106, 2007/02
no abstracts in English
to heavy ion beam irradiationSakashita, Tetsuya; Hamada, Nobuyuki*; Suzuki, Michiyo*; Kakizaki, Takehiko; Wada, Seiichi*; Funayama, Tomoo; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 108, 2007/02
