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Journal Articles

Crystal structure, photomagnetic and dielectric properties of a cyanido-bridged Cu-Mo assembly film

Ikeda, Yusuke*; Matsumura, Daiju; Tsuji, Takuya; Namai, Asuka*; Imoto, Kenta*; Tokoro, Hiroko*; Nakabayashi, Koji*; Okoshi, Shinichi*

Inorganica Chimica Acta, 550, p.121434_1 - 121434_8, 2023/03

 Times Cited Count:0 Percentile:0.01(Chemistry, Inorganic & Nuclear)

Journal Articles

Updating HBase/Hadoop in the J-PARC operation data archiver with making the data collection tool redundant by ZooKeeper

Ikeda, Hiroshi; Kikuzawa, Nobuhiro; Yoshii, Akinobu*; Kato, Yuko

Proceedings of 13th Annual Meeting of Particle Accelerator Society of Japan (Internet), p.637 - 640, 2016/11

no abstracts in English

Journal Articles

The Role of nitric oxide in radiation-induced bystander cell-killing effect

Yokota, Yuichiro; Funayama, Tomoo; Ikeda, Hiroko; Sakashita, Tetsuya; Suzuki, Michiyo; Kobayashi, Yasuhiko

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 67, 2016/02

The role of nitric oxide (NO) in bystander effect was investigated. Human fibroblasts were irradiated with $$gamma$$-rays (LET: 0.2 keV/$$mu$$m) or carbon-ion beam (108 keV/$$mu$$m), and then, co-cultured with the non-irradiated cells. After 24 h culture, the survival rates of non-irradiated cells and the concentrations of nitrate, an oxide of NO, in the medium were measured. The survival rates of non-irradiated cells decreased in dose-dependent and radiation quality-independent manners. Negative relationships between survival rates and nitrite concentrations existed, indicating the amounts of produced NO are an important determinant of bystander effects. Next, a reagent producing two molecules of NO in a half-life of 100 min was added in the culture medium. After incubation of 24 h the survival rates of treated cells did not decrease, suggesting NO produced intracellularly has an important role to lead the bystander effect but is not the signal molecule for intercellular communication.

Journal Articles

Characteristics of radiation-induced bystander effect; Participation of nitric oxide

Yokota, Yuichiro; Funayama, Tomoo; Ikeda, Hiroko; Kobayashi, Yasuhiko

Isotope News, (741), p.21 - 25, 2016/01

Our article published on the International Journal of Radiation Biology (2015) was reviewed. We investigated the dependence of the bystander cell-killing effect on radiation dose and quality, and related molecular mechanisms. Human fibroblasts were irradiated with $$gamma$$-rays or carbon ions and co-cultured with non-irradiated cells. Survival rates of non-irradiated cells decreased and nitrite concentrations in co-culture medium increased with dose. Their dose responses were similar between $$gamma$$-rays and carbon ions. Treatment of the specific nitric oxide (NO) radical scavenger prevented reductions in survival rates of non-irradiated cells. Negative relationships were observed between survival rates and nitrite concentrations. From these results, it was concluded that the bystander cell-killing effect mediated by NO radicals depends on irradiation doses, but not on radiation quality. NO radical production appears to be an important determinant of bystander effects.

Journal Articles

Development of the J-PARC time-series data archiver using a distributed database system, 2

Kikuzawa, Nobuhiro; Ikeda, Hiroshi; Kato, Yuko; Yoshii, Akinobu*

Proceedings of 15th International Conference on Accelerator and Large Experimental Physics Control Systems (ICALEPCS 2015) (Internet), p.818 - 821, 2016/00

J-PARC (Japan Proton Accelerator Research Complex) consists of much equipment. In Linac and 3 GeV rapid cycling synchrotron ring (RCS), the data of over the 64,000 EPICS records for these equipment has been collected. The Data volume will be about 10 TB in 2020. The data have been being stored by a Relational Data Base (RDB) system using PostgreSQL, but it is not enough in availability, performance, and capability to increase of data volume flexibility. Hadoop/HBase, which is known as a distributed, scalable and big data store, has been proposed for our next-generation archive system to solve these problems. The archiving system was built and verified about data transition or database utilization. This report shows the result of the modification of the archive system.

Journal Articles

Improvement of the J-PARC operation data archiver using HBase/Hadoop

Ikeda, Hiroshi; Kikuzawa, Nobuhiro; Yoshii, Akinobu*; Kato, Yuko

Proceedings of 12th Annual Meeting of Particle Accelerator Society of Japan (Internet), p.1312 - 1316, 2015/09

The Linac and the RCS in J-PARC provide enormous operation data and we have been stored them into PostgreSQL. We are planning to replace it with HBase, a data store specialized for big-data and is on a distributed file system provided by Hadoop, using a cluster with advantages including automatically recovering and easily extending the capacity. In the previous presentation we reported we updated HBase/Hadoop in our test system and we showed issues to fix our tools in the new system, while we also mentioned issues about the construction of our cluster itself. In this presentation we are reporting we have re-designed and re-constructed the cluster with resolving the issues, including enhancing hardware of master nodes, creating automatic scripts to construct nodes, and introducing monitoring tools for nodes. Having accordingly adjusted the configurations of HBase/Hadoop and measured the performance of our new system, we are also reporting its results and considerations.

Journal Articles

The Bystander cell-killing effect mediated by nitric oxide in normal human fibroblasts varies with irradiation dose but not with radiation quality

Yokota, Yuichiro; Funayama, Tomoo; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Yasuhiko

International Journal of Radiation Biology, 91(5), p.383 - 388, 2015/05

 Times Cited Count:9 Percentile:64.82(Biology)

We investigated the dependence of the bystander cell-killing effect on radiation dose and quality, and related molecular mechanisms. Human fibroblasts were irradiated with $$gamma$$-rays or carbon ions and co-cultured with non-irradiated cells. Survival rates of non-irradiated cells decreased and nitrite concentrations in culture medium increased with increasing doses. Their dose responses were similar between $$gamma$$-rays and carbon ions. Treatment of the specific nitric oxide (NO) radical scavenger prevented reductions in survival rates of non-irradiated cells. Negative relationships were observed between survival rates and nitrite concentrations. From these results, it was concluded that the bystander cell-killing effect mediated by NO radicals in human fibroblasts depends on irradiation doses, but not on radiation quality. NO radical production appears to be an important determinant of $$gamma$$-ray- and carbon-ion-induced bystander effects.

Journal Articles

Responses of the salt chemotaxis learning in ${it C. elegans}$ mutants to microbeam irradiation

Sakashita, Tetsuya; Suzuki, Michiyo; Hattori, Yuya; Ikeda, Hiroko; Muto, Yasuko*; Yokota, Yuichiro; Funayama, Tomoo; Hamada, Nobuyuki*; Shirai, Kana*; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 74, 2015/03

An increasing body of data indicates that ionizing radiation affects the nervous system and alters its function. Recently, we reported that chemotaxis of ${it C. elegans}$ during the salt chemotaxis learning (SCL), that is conditioned taste aversion to NaCl, was modulated by carbon ion irradiation, i.e. accelerated decrease in chemotaxis to NaCl during the SCL. However, we had no direct evidence for the interaction of ionizing radiation with the central neuronal tissue (nerve ring) in ${it C. elegans}$. Microbeam irradiation is useful to analyze direct radiation effects at a cellular or tissue level. Thus, we applied the microbeam irradiation of the ${it C. elegans}$ nerve ring and examined the effect on the SCL.

Journal Articles

Effects of carbon-ion microbeam irradiation on locomotion and pharyngeal pumping motion in $textit{C. elegans}$

Suzuki, Michiyo; Hattori, Yuya; Sakashita, Tetsuya; Funayama, Tomoo; Yokota, Yuichiro; Ikeda, Hiroko; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 88, 2015/03

Journal Articles

Bystander effect mediated by nitric oxide depends on irradiation dose but not on radiation quality

Yokota, Yuichiro; Funayama, Tomoo; Ikeda, Hiroko; Sakashita, Tetsuya; Suzuki, Michiyo; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 75, 2015/03

We investigated the bystander effect induced by $$gamma$$-rays or carbon ions and analyzed the role of nitric oxide (NO) in the effect. Normal human fibroblasts were used. Cells inoculated on a porous membrane were irradiated with varying doses of $$gamma$$-rays or carbon ions. Irradiated cells were then non-contact co-cultured with non-irradiated cells for 24 h. After co-culture, the survival rates of non-irradiated bystander cells co-cultured with irradiated cells decreased with increasing dose and bottomed out at 0.5 Gy or higher doses. This indicates that the bystander effect is dependent on irradiation dose but independent of radiation quality. Next, a specific NO scavenger c-PTIO was added to the culture medium during irradiation and co-culture. This treatment prevented the reduction in survival rates of bystander cells, clearly indicating that NO has an important role in the bystander effect.

Journal Articles

Mechanisms for the induction of radioadaptive response by radiation-induced bystander response

Matsumoto, Hideki*; Tomita, Masanori*; Otsuka, Kensuke*; Hatashita, Masanori*; Maeda, Munetoshi*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Ikeda, Hiroko; et al.

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 76, 2015/03

The objective of this project is to elucidate molecular mechanisms for the induction of radioadaptive response through radiation-induced bystander responses induced by irradiation with heavy ion microbeams in JAEA. We found that the adaptive response was induced by Ar (520 MeV $$^{40}$$Ar$$^{14+}$$) microbeam-irradiation of a limited number of cells, followed by the broad beam-irradiation and that the adaptive response was almost completely suppressed by the addition of carboxy-PTIO, as a nitric oxide (NO) scavenger. In addition, we found several genes induced specifically and preferentially when radioadaptive response could be induced. We confirmed that ${it iNOS}$ expression was specifically induced only when radioadaptive response could be induced. Our findings strongly suggested that radioadaptive response can be induced by NO-mediated bystander responses evoked by irradiation with heavy ion microbeams.

Journal Articles

Ion-species dependent bystander mutagenic effect on ${it HPRT}$ locus in normal human fibroblasts induced by C-, Ne- and Ar-ion microbeams

Suzuki, Masao*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Suzuki, Michiyo; Ikeda, Hiroko; Hattori, Yuya; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 78, 2015/03

We have been studying the radiation-quality dependent bystander cellular effects, such as cell killing, mutation induction and chromosomal damage, using heavy-ion microbeams with different ion species. This year we focused on the ion-species dependent bystander mutagenic effect on ${it HPRT}$ locus in normal human fibroblasts. The confluent culture were irradiated using a 256 (16$$times$$16)-cross-stripe method using C, Ne and Ar microbeam. Gene mutation on ${it HPRT}$ locus was detected with 6-thioguanine resistant clones. The mutation frequency in cells irradiated with C-ion microbeams was 6 times higher than that of non-irradiated control cells and of the sample treated with specific inhibitor of gap-junction cell-to-cell communication. On the other hand, no enhanced mutation frequencies were observed in cells irradiated with either Ne- or Ar-ion microbeams. There is clear evidence that the bystander mutagenic effect via gap-junction communication depends on radiation quality.

Journal Articles

Medaka blastoderm cells are capable of compensating the injured cells irradiated by carbon-ion micro-beam

Yasuda, Takako*; Oda, Shoji*; Asaka, Tomomi*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Yasuhiko; Mitani, Hiroshi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 85, 2015/03

In this present study, we examined the effects of heavy carbon-ions on development in pre-implantation period utilizing medaka blastula stage embryos (st. 11: blastderm diameter is about 500 $$mu$$m). We performed targeted irradiation by carbon-ion micro-beam (diameters of 120, 180 $$mu$$m) to a central parts of blastoderm and observed the abnormalities during development compared with whole-body irradiated embryos. As a results, retardation and characteristic malformed eyes were observed during development when blastoderm cells were partially irradiated, However, more than half of 50 Gy-irradiated embryos (area size=120 $$mu$$m diameter) could hatch normally in contrast to all embryos with 2 Gy of whole-body irradiation being lethal before hutching.

Journal Articles

Status of operation data archiving system using Hadoop/HBase for J-PARC

Kikuzawa, Nobuhiro; Ikeda, Hiroshi; Kato, Yuko; Ouchi, Nobuo; Yoshii, Akinobu*

Proceedings of 10th International Workshop on Personal Computers and Particle Accelerator Controls (PCaPAC 2014) (Internet), p.193 - 195, 2015/02

J-PARC (Japan Proton Accelerator Research Complex) consists of much equipment. In Linac and 3 GeV rapid cycling synchrotron ring (RCS), the data of over the 64,000 EPICS records for these equipment has been collected. The Data volume is about 2 TB in every year, and the stored total data volume is about 10 TB. The data have been being stored by a Relational Data Base (RDB) system using PostgreSQL, but it is not enough in availability, performance, and capability to increase of data volume flexibility. Hadoop/HBase, which is known as a distributed, scalable and big data store, has been proposed for our next-generation archive system to solve these problems. The test system was built and verified about data transition or database utilization. This report shows the current status of the new archive system, and its advantages and problems which have been obtained through our verification.

Journal Articles

Development of tools for the J-PARC operation data archiving using HBase/Hadoop

Kikuzawa, Nobuhiro; Ikeda, Hiroshi; Yoshii, Akinobu*; Kato, Yuko; Ouchi, Nobuo

Proceedings of 11th Annual Meeting of Particle Accelerator Society of Japan (Internet), p.794 - 798, 2014/10

no abstracts in English

Journal Articles

Present status of J-PARC linac

Oguri, Hidetomo; Hasegawa, Kazuo; Ito, Takashi; Chishiro, Etsuji; Hirano, Koichiro; Morishita, Takatoshi; Shinozaki, Shinichi; Ao, Hiroyuki; Okoshi, Kiyonori; Kondo, Yasuhiro; et al.

Proceedings of 11th Annual Meeting of Particle Accelerator Society of Japan (Internet), p.389 - 393, 2014/10

no abstracts in English

Journal Articles

Status of J-PARC operation data archiving using Hadoop and HBase

Kikuzawa, Nobuhiro; Yoshii, Akinobu; Ikeda, Hiroshi; Kato, Yuko

Proceedings of 10th Annual Meeting of Particle Accelerator Society of Japan (Internet), p.230 - 234, 2014/06

J-PARC is consists of much equipment. In Linac and 3GeV synchrotron, the data of over the 64,000 EPICS records for these apparatus control is being collected. The data has been being stored by a RDB system using PostgreSQL now, but it is not enough in availability, performance, and extendibility. Therefore, the new system architecture is required, which is rich in the pliability and can respond to the data increasing continuously for years to come. In order to cope with this problem, we considered adoption of the distributed database architecture and constructed the demonstration system using HBase. We present results of these demonstration.

Journal Articles

Radiation-quality-dependent bystander effects induced by the microbeams with different radiation sources

Suzuki, Masao*; Autsavapromporn, N.*; Usami, Noriko*; Funayama, Tomoo; Plante, I.*; Yokota, Yuichiro; Muto, Yasuko*; Suzuki, Michiyo; Ikeda, Hiroko; Hattori, Yuya; et al.

Journal of Radiation Research, 55(Suppl.1), P. i54, 2014/03

Journal Articles

Development of J-PARC time-series data archiver using distributed database system

Kikuzawa, Nobuhiro; Yoshii, Akinobu; Ikeda, Hiroshi; Kato, Yuko

Proceedings of 14th International Conference on Accelerator and Large Experimental Physics Control Systems (ICALEPCS 2013) (Internet), p.584 - 586, 2013/10

J-PARC is consists of much equipment. In Linac and 3 GeV synchrotron, the data of over the 64,000 EPICS records about these apparatus control has been collected. The data has been being stored by a RDBMS (Rerational Data Base Management System) using Postgre SQL now, but it is not enough in availability, performance, and extendibility. Therefore, we think that the new system architecture excellent in the pliability will be required, which is rich in the pliability and can respond to the data which continues increasing for years to come. In order to deal with this problem, we considered use of distributed database system and constructed the demonstration system using HBase. We present results of these demonstration.

Journal Articles

Gap junction communication and the propagation of bystander effects induced by microbeam irradiation in human fibroblast cultures; The Impact of radiation quality

Autsavapromporn, N.*; Suzuki, Masao*; Funayama, Tomoo; Usami, Noriko*; Plante, I.*; Yokota, Yuichiro; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Katsumi*; Kobayashi, Yasuhiko; et al.

Radiation Research, 180(4), p.367 - 375, 2013/10

 Times Cited Count:57 Percentile:89.96(Biology)

We investigated the role of gapjunction intercellular communication (GJIC) in the propagation of stressful effects in confluent normal human fibroblast cultures wherein only 0.036-0.144% of cells in the population were traversed by primary radiation tracks. Confluent cells were exposed to graded doses from X ray, carbon ion, neon ion or argon ion microbeams in the presence or absence of an inhibitor of GJIC. After 4 h incubation, the cells were assayed for micronucleus (MN) formation. Micronuclei were induced in a greater fraction of cells than expected based on the fraction of cells targeted by primary radiation, and the effect occurred in a dose-dependent manner with any of the radiation sources. Interestingly, the inhibition of GJIC depressed the enhancement of MN formation in bystander cells from cultures exposed to high-LET radiation but not low-LET radiation. The results highlight the important role of radiation quality and dose in the observed effects.

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