Behavioral resistance of against high-LET radiation exposure

Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Shimozawa, Yoko; Fukamoto, Kana*; Yokota, Yuichiro; Sora, Sakura*; Kakizaki, Takehiko*; Wada, Seiichi*; Funayama, Tomoo; et al.

Biological Sciences in Space, 26, p.7 - 11, 2012/07

https://doi.org/10.2187/bss.26.7

Here, we investigated the resistance to high-LET radiation exposure for two behaviors of the nematode, , which is known as a model organism for the nervous system. Tested behaviors were locomotion and chemotaxis to NaCl. In addition, egg hatchability was examined as an indicator of high-LET radiation sensitivity. Relative biological effectiveness (RBE) of high-LET radiation (C, 18.3 MeV/u, LET = 113 keV/m) relative to low-LET radiation for hatchability was 4.5, whereas RBEs for locomotion and chemotaxis were 1.4 and 1.1, respectively. This study shows that the behavioral system for locomotion and chemotaxis of is highly resistant to high-LET radiation exposure.

Targeted heavy-ion microbeam irradiation of the embryo but not yolk in the diapause-terminated egg of the silkworm, , induces the somatic mutation

Furusawa, Toshiharu*; Fukamoto, Kana*; Sakashita, Tetsuya; Suzuki, Eiko*; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Funayama, Tomoo; Suzuki, Hiromi*; Ishioka, Noriaki*; Wada, Seiichi*; et al.

Journal of Radiation Research, 50(4), p.371 - 375, 2009/07

https://doi.org/10.1269/jrr.09021

Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, . On one hand, carbon-ion exposure of embryo to 0.5 - 6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.

Insufficient membrane fusion in dysferlin-deficient muscle fibers after heavy-ion irradiation

Hino, Mizuki*; Hamada, Nobuyuki*; Tajika, Yuki*; Funayama, Tomoo; Morimura, Yoshihiro*; Sakashita, Tetsuya; Yokota, Yuichiro; Fukamoto, Kana*; Kobayashi, Yasuhiko; Yorifuji, Hiroshi*

Cell Structure and Function, 34(1), p.11 - 15, 2009/03

https://doi.org/10.1247/csf.08033

In vivo cyst formation from the implanted larval integument in the sweet potato hornworm, , as a simple model for studing wound healing

Fukamoto, Kana

Sanshi, Konchu Biotekku, 77(1), p.17 - 18, 2008/04

no abstracts in English

Influence of local irradiation with heavy-ion microbeam on the incidence of somatic mutation arising on the larvae in embryo and yolk in the egg of the silkworm,

Furusawa, Toshiharu*; Suzuki, Eiko*; Nagaoka, Shunji*; Suzuki, Hiromi*; Ishioka, Noriaki*; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko; Sakashita, Tetsuya; Kakizaki, Takehiko*; et al.

JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 115, 2008/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2007-060.pdf

Using heavy ion microbeam, we investigated the somatic mutation arising on the larvae in embyro and yolk in the egg of silkworm, . The incidence of the somatic mutation was 12%, and the same level of mutation following the microbeam irradiation at the center of the egg. However, the microbeam irradiation to the abdomen of the silkworm larvae induced the increase of somatic mutation, 63% (3 Gy) and 80% (6 Gy).

Development of the irradiation method for the first instar silkworm larvae using locally targeted heavy-ion microbeam

Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.

Journal of Radiation Research, 48(3), p.247 - 253, 2007/05

https://doi.org/10.1269/jrr.06066

To carry out the radio-microsurgery study using silkworm, , we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon (C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.

Regeneration mechanism of hemopoietic organs in the silkworm, , after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strain

Kiguchi, Kenji*; Shirai, Koji*; Sakata, Toshiyuki*; Fukamoto, Kana; Kakizaki, Takehiko; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 117, 2007/02

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2006-042.pdf

no abstracts in English

Effects of heavy-ion irradiation on the differentiation of epidermal cells in the silkworm,

Fukamoto, Kana; Shimura, Sachiko*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.107 - 114, 2006/10

no abstracts in English

LET dependence of radiation-induced delayed reproductive death in normal human diploid cells

Hamada, Nobuyuki*; Hara, Takamitsu*; Funayama, Tomoo; Sakashita, Tetsuya; Wada, Seiichi*; Kakizaki, Takehiko; Suzuki, Michiyo; Fukamoto, Kana; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Inhibition of abnormal proliferation of epidermal cells in the knobbed mutant silkworm larva by the heavy-ion microbeam irradiation

Fukamoto, Kana; Sakata, Toshiyuki*; Shirai, Koji*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; Hara, Takamitsu*; Suzuki, Michiyo*; et al.

no journal, , 

Silkworm is an experimental insect good to investigate developmental biology or cell differentiation. Knobbed mutant is a quite unique and important model of cell differentiation, in that cells in the knob region consist of abnormally proliferated and stratified cells. In this study, the new application of irradiation with heavy ion microbeam for the first instar silkworm larvae was developed to clarify that when and where the knob mutant would form abnormal proliferation of epidermal cells. The holed aluminum plates were designed to fix the first instar larvae of silkworm during irradiation. After carbon ions microbeam irradiation, larvae were reared to evaluate the accuracy of irradiation. The deletion of knob was observed in over 70% of the larvae at fifth instar. The epidermal cells stayed, as it was a monolayer at irradiated region. These results indicate that heavy ion beam irradiation can control the abnormal cell division of epidermis in the knob mutant.