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Journal Articles

Development of the irradiation method for the first instar silkworm larvae using locally targeted heavy-ion microbeam

Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.

Journal of Radiation Research, 48(3), p.247 - 253, 2007/05

https://doi.org/10.1269/jrr.06066
 Times Cited Count：17 Percentile：47.71(Biology)

To carry out the radio-microsurgery study using silkworm, ${it Bombyx mori}$, we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon ($$^{12}$$C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.

Journal Articles

Regeneration mechanism of hemopoietic organs in the silkworm, ${it Bombyx mori}$, after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strain

Kiguchi, Kenji*; Shirai, Koji*; Sakata, Toshiyuki*; Fukamoto, Kana; Kakizaki, Takehiko; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 117, 2007/02

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2006-042.pdf

no abstracts in English

Journal Articles

Process of ${it in vivo}$ cyst formation from the implanted larval integument in the sweet potato hornworm, ${it Agrius convolvuli}$; A Simple model for studying wound healing

Fukamoto, Kana; Shirai, Koji*; Sato, Shigeru*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.99 - 106, 2006/10

no abstracts in English

Journal Articles

Effects of heavy-ion irradiation on the differentiation of epidermal cells in the silkworm, ${it Bombyx mori}$

Fukamoto, Kana; Shimura, Sachiko*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.107 - 114, 2006/10

no abstracts in English

Journal Articles

Contribution of circulating hemocytes to the regeneration of heavy ion beams ($$^{12}$$C$$^{5+}$$) irradiated hematopoietic organs in the silkworm, ${it Bombyx mori}$, through the way of phagocytosis of injured cells after invasion

Ling, E.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Funayama, Tomoo; Watanabe, Hiroshi*

Developmental and Comparative Immunology, 30(6), p.531 - 543, 2006/00

https://doi.org/10.1016/j.dci.2005.08.003
 Times Cited Count：13 Percentile：54.26(Fisheries)

no abstracts in English

Journal Articles

Regeneration of hemopoietic organs in the silkworm, ${it bombyx mori}$, after locally targeted irradiation with heavy ion beams

Ling, E.*; Fukamoto, Kana*; Xu, S.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*

Journal of Insect Biotechnology and Sericology, 72(2), p.95 - 100, 2003/09

no abstracts in English

Journal Articles

Why does hemocyte density rise during the wandering stage of the silkworm, ${it bombyx mori}$?

Ling, E.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*

Journal of Insect Biotechnology and Sericology, 72(2), p.101 - 109, 2003/09

no abstracts in English

Journal Articles

Effects of local irradiation of heavy ion beams to the hematopoietic organs of the silkworm, Bombyx mori

Tu, Z. L.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

Nihon Sanshigaku Zasshi, 68(6), p.491 - 500, 1999/00

https://doi.org/10.11416/kontyushigen1930.68.491

no abstracts in English

Journal Articles

Effects of general and local irradiation of heavy ion beams on the development and morphogenesis of the silkworm, Bombyx mori

Tu, Z. L.*; Yamasaki, Shuhei*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi

Nihon Sanshigaku Zasshi, 68(6), p.443 - 453, 1999/00

https://doi.org/10.11416/kontyushigen1930.68.443

no abstracts in English

Oral presentation

Analysis of the regeneration mechanism of heavy-ion irradiated hematopoietic organs in the silkworm

Sakata, Toshiyuki*; Shirai, Koji*; Kiguchi, Kenji*; Fukamoto, Kana; Kakizaki, Takehiko; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Oral presentation

Inhibition of abnormal proliferation of epidermal cells in the knobbed mutant silkworm larva by the heavy-ion microbeam irradiation

Fukamoto, Kana; Sakata, Toshiyuki*; Shirai, Koji*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; Hara, Takamitsu*; Suzuki, Michiyo*; et al.

no journal, , 

Silkworm is an experimental insect good to investigate developmental biology or cell differentiation. Knobbed mutant is a quite unique and important model of cell differentiation, in that cells in the knob region consist of abnormally proliferated and stratified cells. In this study, the new application of irradiation with heavy ion microbeam for the first instar silkworm larvae was developed to clarify that when and where the knob mutant would form abnormal proliferation of epidermal cells. The holed aluminum plates were designed to fix the first instar larvae of silkworm during irradiation. After carbon ions microbeam irradiation, larvae were reared to evaluate the accuracy of irradiation. The deletion of knob was observed in over 70% of the larvae at fifth instar. The epidermal cells stayed, as it was a monolayer at irradiated region. These results indicate that heavy ion beam irradiation can control the abnormal cell division of epidermis in the knob mutant.

Oral presentation

Inhibition of abnormal proliferation of epidermal cells in the knobbed mutant silkworm larva by the heavy-ion microbeam irradiation

Fukamoto, Kana; Sakata, Toshiyuki*; Shirai, Koji*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; Hara, Takamitsu*; Suzuki, Michiyo; et al.

no journal, , 

no abstracts in English

Oral presentation

Regeneration of heavy-ion exposed hematopoietic forming organ in silkworm, ${it Bombyx mori}$

Sakata, Toshiyuki*; Shirai, Koji*; Tsuchiya, Shiori*; Kiguchi, Kenji*; Fukamoto, Kana; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Sato, Shigeru*

no journal, , 

no abstracts in English

Oral presentation

Effects of $$gamma$$ rays on the cell line SS4 of a silkworm, ${it Bombyx. mori}$

Fukamoto, Kana; Tsuchiya, Shiori*; Shirai, Koji*; Kiguchi, Kenji*; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Imanishi, Shigeo*

no journal, , 

no abstracts in English

Oral presentation

Effects of $$gamma$$ irradiation on cell proliferation of the newly established cell line, SS4

Fukamoto, Kana; Tsuchiya, Shiori*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Suzuki, Michiyo*; Kakizaki, Takehiko*; Wada, Seiichi*; Hara, Takamitsu*; Imanishi, Shigeo*; Shirai, Koji*; et al.

no journal, , 

no abstracts in English

Oral presentation

Post-translational processing of epidermal carotenoid-binding protein at the larval epidermis of ${it Agrius convolvuli}$

Shirai, Koji*; Sumita, Yoshinobu*; Fukushima, Hisato*; Fukamoto, Kana; Kiguchi, Kenji*

no journal, , 

no abstracts in English

Oral presentation

Regeneration mechanisms of hemopoietic organs in the silkworm, ${it Bombyx mori}$, after heavy-ion irradiation; Detection and identification of the stage-specific marker

Kobayashi, Satoshi*; Sakata, Toshiyuki*; Tsuchiya, Shiori*; Shirai, Koji*; Kiguchi, Kenji*; Fukamoto, Kana; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Oral presentation

Effects of heavy-ion irradiation on the insect culture cell; Sf9 examination of the phosphorylation of eukaryotic initiation factor 2 alfa

Tsuchiya, Shiori*; Fukamoto, Kana; Shirai, Koji*; Kiguchi, Kenji*; Funayama, Tomoo; Sakashita, Tetsuya; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Oral presentation

Low dose heavy-ion irradiation is enough to stop the hemopoiesis in the hemopoietic organs of the silkworm, ${it Bombyx mori}$

Fukamoto, Kana; Tsuchiya, Shiori*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Shirai, Koji*; Kiguchi, Kenji*; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Oral presentation

Regeneration mechanism of hemopoietic organs in the silkworm, ${it Bombyx mori}$, after heavy-ion irradiation; Analysis by transplantation of the irradiated organs using a transgenic silkworm strain

Shirai, Koji*; Kiguchi, Kenji*; Sakata, Toshiyuki*; Fukamoto, Kana; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko

no journal, , 

22 (Records 1-20 displayed on this page)