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Journal Articles

Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays

Noguchi, Miho; Kanari, Yukiko; Yokoya, Akinari; Narita, Ayumi; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.101 - 103, 2015/09

 Times Cited Count:1 Percentile:9.79(Environmental Sciences)

Journal Articles

Real-time observation of irradiated HeLa-cell modified by fluorescent ubiquitination-based cell-cycle indicator using synchrotron X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.192 - 196, 2015/09

 Times Cited Count:3 Percentile:26.04(Environmental Sciences)

For the knowledge about irradiation effects of mammalian cells depending on the cell cycle, most of them had been analyzed by statistical approches. Our purpose is to establish the method for real-time observation of irradiated cells under a microscope. Fluorescent ubiquitination-based cell cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were selectively irradiated according to cell color observed in the same microscopic field in a culture dish. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. The cell cycle was strongly arrested by irradiation at S/G2 and never progressed to G1. In contrast, cells irradiated at G1 progress to S/G2 with a similar time course as non-irradiated control cells. These results show single FUCCI cell exposure and live cell imaging are powerful methods for studying radiation effects on the cell cycle.

Journal Articles

A Novel technique using DNA denatureation to detect multiplly induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and $$^{4}$$He$$^{2+}$$ ion irradiation

Yokoya, Akinari; Shikazono, Naoya; Fujii, Kentaro; Noguchi, Miho; Urushibara, Ayumi

Radiation Protection Dosimetry, 143(2-4), p.219 - 225, 2011/02

 Times Cited Count:3 Percentile:26.09(Environmental Sciences)

Multiple single-strand breaks (m-SSBs), which are predicted to be preferentially induced by high LET radiation, would be underestimated if one uses the conventional method using plasmid DNA, because m-SSBs will not cause additionally conformational changes if they are on the same or on the opposite strand but separated each other sufficiently so as not to induce a double strand break. In order to observe the invisible m-SSBs, we have developed a novel technique using DNA denaturation. The m-SSBs arising in both strands of DNA are revealed as molecular size change in single strand DNA (SS-DNA) by gel electrophoresis. We have applied this method to the X- and He$$^{2+}$$ ion irradiated sample of hydrated pUC18 plasmid DNA. A half of SS-DNA population remains as intact within the experimental resolution ($$<$$ 140 bases) for both irradiations. Contrary to our initial expectation, these results indicate that SSBs are not multiply induced over 140 bp even by high-LET irradiation.

Journal Articles

A Model for analysis of the yield and the level of clustering of radiation-induced DNA-strand breaks in hydrated plasmids

Shikazono, Naoya; Yokoya, Akinari; Urushibara, Ayumi; Noguchi, Miho; Fujii, Kentaro

Radiation Protection Dosimetry, 143(2-4), p.181 - 185, 2011/02

 Times Cited Count:2 Percentile:18.37(Environmental Sciences)

Journal Articles

The Yield, processing, and biological consequences of clustered DNA damage induced by ionizing radiation

Shikazono, Naoya; Noguchi, Miho; Fujii, Kentaro; Urushibara, Ayumi*; Yokoya, Akinari

Journal of Radiation Research, 50(1), p.27 - 36, 2009/01

 Times Cited Count:112 Percentile:88.08(Biology)

After living cells are exposed to ionizing radiation, a variety of chemical modifications of DNA are induced either directly by ionization of DNA or indirectly through interactions with water-derived radicals. Clustered DNA damage, which is defined as two or more of such lesions within one to two helical turns of DNA induced by a single radiation track, is considered to be a unique feature of ionizing radiation. A double strand break (DSB) is a type of clustered DNA damage. Formation and repair of DSBs have been studied in great detail over the years as they have been linked to important biological endpoints. Although non-DSB clustered DNA damage has received less attention, there is growing evidence of its biological significance. This review focuses on the current understanding of (1) the yield of non-DSB clustered damage induced by ionizing radiation (2) the processing, and (3) biological consequences of non-DSB clustered DNA damage.

Journal Articles

Laser prepulse dependency of proton-energy distributions in ultraintense laser-foil interactions with an online time-of-flight technique

Yogo, Akifumi; Daido, Hiroyuki; Fukumi, Atsushi*; Li, Z.*; Ogura, Koichi; Sagisaka, Akito; Pirozhkov, A. S.; Nakamura, Shu*; Iwashita, Yoshihisa*; Shirai, Toshiyuki*; et al.

Physics of Plasmas, 14(4), p.043104_1 - 043104_6, 2007/04

 Times Cited Count:62 Percentile:88.22(Physics, Fluids & Plasmas)

Fast protons are observed by a newly-developed $textit{online}$ time-of-flight spectrometer, which provides $textit{shot-to-shot}$ proton-energy distributions immediately after the irradiation of a laser pulse having an intensity of $$sim 10^{18}$$ W/cm$$^2$$ onto a 5-$$mu$$m-thick copper foil. The maximum proton energy is found to increase when the intensity of a fs-prepulse arriving 9 ns before the main pulse increases from 10$$^{14}$$ to 10$$^{15}$$ W/cm$$^2$$. Interferometric measurement indicates that the preformed-plasma expansion at the front surface is smaller than 15 $$mu$$m, which corresponds to the spatial resolution of the diagnostics. This sharp gradient of the plasma makes a beneficial effect on increasing the absorption efficiency of the main-pulse energy, resulting in the increase in the proton energy. This is supported by the result that the X-ray intensity from the laser plasma clearly increases with the prepulse intensity.

Journal Articles

Natural circular dichroism of amino acid films observed in soft X-ray and VUV region using polarizing undulator

Nakagawa, Kazumichi*; Kaneko, Fusae*; Ota, Yoshimi*; Tanaka, Masahito*; Kitada, Tomo*; Agui, Akane; Fujii, Kentaro; Yokoya, Akinari; Watanabe, Kazutoshi*; Yamada, Toru*

Journal of Electron Spectroscopy and Related Phenomena, 144-147, p.271 - 273, 2005/06

 Times Cited Count:12 Percentile:50.42(Spectroscopy)

no abstracts in English

JAEA Reports

Vectorization, parallelization and porting of nuclear codes on the VPP500 system, porting; Progress report fiscal 1996

*; *; *; *; *; *; Harada, Hiro; ; Kume, Etsuo;

JAERI-Data/Code 97-055, 161 Pages, 1998/01

JAERI-Data-Code-97-055.pdf:4.57MB

no abstracts in English

JAEA Reports

Vectorization, parallelization and porting of nuclear codes on the VPP500 system (parallelization;Progress report fiscal 1996

*; *; *; *; *; *; Harada, Hiro; ; Kume, Etsuo;

JAERI-Data/Code 97-052, 160 Pages, 1997/12

JAERI-Data-Code-97-052.pdf:4.17MB

no abstracts in English

JAEA Reports

Vectorization, parallelization and porting of nuclear codes on the VPP500 system (vectorization); Progress report fiscal 1996

*; *; *; *; *; *; Harada, Hiro; ; Kume, Etsuo;

JAERI-Data/Code 97-051, 142 Pages, 1997/12

JAERI-Data-Code-97-051.pdf:3.91MB

no abstracts in English

JAEA Reports

AVS user's guide on the basis of practice

*; Kato, Katsumi*; Kume, Etsuo;

JAERI-Tech 97-028, 37 Pages, 1997/07

JAERI-Tech-97-028.pdf:1.32MB

no abstracts in English

Journal Articles

A New algorithm for resolving position reverse problems in stereo matching using dynamic programming

; *

Denshi Joho Tsushin Gakkai Rombunshi, D-II, J79-D-2(5), p.775 - 784, 1996/05

no abstracts in English

Journal Articles

Studies on the position reverse problem in stereo matching using dynamic programming methods

Ibaraki Daigaku Hakase Rombun, 0, 82 Pages, 1996/03

no abstracts in English

Journal Articles

Numerical analysis of buoyancy-driven exchange flow with regard to an HTTR air ingress accident

Fumizawa, Motoo; Kunugi, Tomoaki; Hishida, Makoto; *; Fujii, Sadao*; Igarashi, Minoru*

Nuclear Technology, 110, p.263 - 272, 1995/05

 Times Cited Count:4 Percentile:43.25(Nuclear Science & Technology)

no abstracts in English

JAEA Reports

Vectorization and improvement of nuclear codes, III: DGR, STREAM V3.1, Cella, GGR

; *; *; *; Yokokawa, Mitsuo;

JAERI-Data/Code 94-021, 119 Pages, 1995/01

JAERI-Data-Code-94-021.pdf:3.08MB

no abstracts in English

Journal Articles

High performance computing at Japan Atomic Energy Research Institute

; Higuchi, Kenji; ; Harada, Hiro; ; *; *

High Performance computing 1995; Grand Challenges in Computer Simulation, 0, p.9 - 15, 1995/00

no abstracts in English

JAEA Reports

Study on intelligent nuclear systems(HASP: Human Acts Simulation Program); Progress report, 1992

; Higuchi, Kenji; ; Kume, Etsuo; ; Ebihara, Kenichi; Otani, Takayuki

JAERI-M 94-051, 109 Pages, 1994/03

JAERI-M-94-051.pdf:3.86MB

no abstracts in English

72 (Records 1-20 displayed on this page)