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Fujii, Kentaro; Fujii, Shinichiro*; Akimitsu, Nobuyoshi*; Tsukimoto, Mitsutoshi*; Kojima, Shuji*
no journal, ,
ATP (Adenosine tri-phosphate), one of ribonucleic acids, acts as an intracellular energy transfer. ATP is used as a substrate to synthesized messenger RNA and as a ligand of inter-cellular signaling. SPring-8 beam line BL23SU can produce high resolution monochromatic soft X-rays. In this study, we analyzed the soft X-ray induced radiation damage of ATP by various biological assessments, such as energy donor activity, genetic information transfer activity and inter-cellular signaling activity.
Fujii, Kentaro; Fujii, Shinichiro*; Akimitsu, Nobuyoshi*; Tsukimoto, Mitsutoshi*; Kojima, Shuji*
no journal, ,
We analyze the luciferase activity to assess the energy transfer activity and the genetic information transfer activity and evaluate the change in activity as an inter-cellular signaling molecules by measuring ATP receptor-mediated phosphorylation of extra cellular signal-regulated kinase 1/2 (ERK1/2). In addition to the biological effect, we have also measured the molecular alterations using X-ray absorption and electro spray mass spectrometry (ESIMS). We observed attenuation of ATP receptor-mediated phosphorylation of ERK 1/2 and luciferase activity by the soft X-ray irradiations. Moreover, we observed the reduction of nucleotide from the X-ray absorption spectra and ESIMS. These results suggest that certain alteration of ATP structure induced by soft X-ray affects the biological function of ATP.
Fujii, Kentaro; Fujii, Shinichiro*; Kato, Dai*; Akimitsu, Nobuyoshi*; Tsukimoto, Mitsutoshi*; Yokoya, Akinari; Niwa, Osamu*; Kojima, Shuji*
no journal, ,
no abstracts in English
Fujii, Kentaro; Fujii, Shinichiro*; Kato, Dai*; Tsukimoto, Mitsutoshi*; Akimitsu, Nobuyoshi*; Narita, Ayumi; Nakajima, Takafumi*; Ide, Shunta*; Salam, K. A.*; Niwa, Osamu*; et al.
no journal, ,