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Bughio, N.*; Nakanishi, Hiromi*; Kiyomiya, Shoichiro*; Matsuhashi, Shimpei; Ishioka, Noriko; Watanabe, Satoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; Osa, Akihiko; Kume, Tamikazu; et al.
Planta, 213(5), p.708 - 715, 2001/09
Times Cited Count:16 Percentile:36.85(Plant Sciences)[C]Methionine ([C]Met) was supplied through barley roots and the C signal was follwoed using a real-time imaging system (PETIS), with subsequent development of autoradiographic images of the whole plant. In all cases, [C]Met was first translocated to the discrimination center, and this part was strongly labeled. Met absorbed by roots of the plants was subsequently translocated to other parts of the plant. In Fe-deficient (-Fe) barley plants, a drastic reduction in [C]Met translocation from the roots to the shoot was observed, while a greater amount of C was found in the leaves of Fe-sufficient or Met-pretreated -Fe plants. Treatment of -Fe plants with amiooxyacetic acid increased the translocation of [C]Met to the shoot. The retention of exogenously supplied [C]Met in the roots of -Fe barley indicates that the Met is used in the biosynthesis of mugineic acid (MA) in barley roots. This and the absence of Met movement from shoots to the roots suggest that the MA phytosiderophores precursor Met originates in the roots of plants.
Furukawa, Jun*; Yokota, Harumi*; Tanoi, Keitaro*; Ueoka, Shiori*; Matsuhashi, Shimpei; Ishioka, Noriko; Watanabe, Satoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; Ito, Takehito*; et al.
Journal of Radioanalytical and Nuclear Chemistry, 249(2), p.495 - 498, 2001/08
Times Cited Count:16 Percentile:73.21(Chemistry, Analytical)We present real time Vanadate (V) uptake imaging in a cowpea plant by Positron Emitting Tracer Imaging System (PETIS). Vanadium-48 was produced by bombarding a Sc foil target with 50 MeV -particles at Takasaki Ion accelerators for Advanced Radiation Application (TIARA) AVF cyclotron. Then V was added to the culture solution to investigate the V distribution in a cowpea plant. The real time uptake of the V was monitored by PETIS. We measured the distribution of V in a whole plant after 3, 6 and 20 hours of V treatment by Bio-imaging Analyzer System (BAS). After the 20 hour treatment, vanadate was detected at the up-ground part of the plant. To know the effect of V uptake on plant activity, F-labeled water uptake was analyzed by PETIS. When a cowpea plant was treated with V for 20 hours before F-labeled water uptake experiment, the total amount of F-labeled water absorption was drastically desreased. Results suggest the inhibition of water uptake was mainly caused by the vanadate already moved to the up-ground part of the plant.
Nakanishi, Tomoko*; Tanoi, Keitaro*; Yokota, Harumi*; Kang, D.-J.*; Ishii, Ryuichi*; Ishioka, Noriko; Watanabe, Satoshi; Osa, Akihiko; Sekine, Toshiaki; Matsuhashi, Shimpei; et al.
Journal of Radioanalytical and Nuclear Chemistry, 249(2), p.503 - 507, 2001/08
We present the water uptake ability of cowpea () which has been regarded as one of the most drought resistant species among the pulse crops. It has been suggested that in the lower part of the stem, parenchymatous tissue for storing water had been developed for the function of deought resistance. We confirmed that in this tissue, water amount was high compared to the other stems by neutron radiography. Then the water uptake manner was measured by positron emitting tracer imaging system (PETIS) using F labeled water produced by a cyclotron. Comparing the water uptake manner of cowpea plant with that of common bean, cowpea plant was found to maintain high water uptake activity after drying treatment, suggesting the high drought resistant character.
Nakanishi, Tomoko*; Yokota, Harumi*; Tanoi, Keitaro*; Ikeue, Natsuko*; Okuni, Yoko*; Furukawa, Jun*; Ishioka, Noriko; Watanabe, Satoshi; Osa, Akihiko; Sekine, Toshiaki; et al.
Radioisotopes, 50(6), p.265 - 269, 2001/06
O-Labeled and F-Labeled water uptake manner in a soybean plant was compared by PETIS (Positron Emitting Tracer Imaging System). O-Labeled water (half-life: 2 min) and F-Labeled water (half-life: 110 min) were produced by a cyclotron by N(d,n)O and O(,pn)F reactions, respectively. A root of a soybean plant was cut off and each labeled water was supplied from the basal part of the plant. The gamma-rays emitted from the sample was measured by a BGO counter with a detection area of 5 cm15 cm. The radioactivity from each stem was accumlated every 15 s till 20 min. It was found that F-labeled water was taken up much faster than O-labeled water, suggesting that in F-labeled water, fluorine was moved in the form of F-ion. When BAS image of O-labeled water in a plant after 5 min and 10 min of water supply was taken, it was found that the labeled water was observed only in the lowest internode, between a root and the first leaves.
Mori, Satoshi*; Kiyomiya, Shoichiro*; Nakanishi, Hiromi*; Ishioka, Noriko; Watanabe, Satoshi; Osa, Akihiko; Matsuhashi, Shimpei; Hashimoto, Shoji; Sekine, Toshiaki; Uchida, Hiroshi*; et al.
Soil Science and Plant Nutrition, 46(4), p.975 - 979, 2000/12
Times Cited Count:29 Percentile:57.37(Plant Sciences)no abstracts in English
Nakanishi, Hiromi*; N.Bughio*; Matsuhashi, Shimpei; Ishioka, Noriko; Uchida, Hiroshi*; Tsuji, Atsunori*; Osa, Akihiko; Sekine, Toshiaki; Kume, Tamikazu; Mori, Satoshi*
Journal of Experimental Botany, 50(334), p.637 - 643, 1999/05
no abstracts in English
Ishioka, Noriko; Matsuoka, Hiromitsu; Watanabe, Satoshi; Osa, Akihiko; Koizumi, Mitsuo; Matsuhashi, Shimpei; Kume, Tamikazu; Sekine, Toshiaki; Uchida, Hiroshi*; Tsuji, Atsunori*; et al.
Journal of Radioanalytical and Nuclear Chemistry, 239(2), p.417 - 421, 1999/00
Times Cited Count:27 Percentile:86.12(Chemistry, Analytical)no abstracts in English
Uchida, Hiroshi*; *; *; Tsuji, Atsunori*; *; Fujimura, Takashi; Matsuhashi, Shimpei; Kume, Tamikazu
Hoshasen To Sangyo, (80), p.6 - 10, 1998/00
no abstracts in English
Kume, Tamikazu; Matsuhashi, Shimpei; Ito, Hitoshi; G.W.Roeb*; Ishioka, Noriko; Osa, Akihiko; Matsuoka, Hiromitsu; Sekine, Toshiaki; Uchida, Hiroshi*; Tsuji, Atsunori*; et al.
JAERI-Review 97-015, p.51 - 53, 1997/11
no abstracts in English
Kume, Tamikazu; Fujimura, Takashi; Ishioka, Noriko; Uchida, Hiroshi*; Tsuji, Atsunori*
Nature, 389(6649), 1 Pages, 1997/09
no abstracts in English
Kume, Tamikazu; Matsuhashi, Shimpei; *; Ito, Hitoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; ; Matsuoka, Hiromitsu; Osa, Akihiko; Sekine, Toshiaki
Plant Nutrition for Sustainable Food Production and Environment, 0, p.169 - 170, 1997/00
no abstracts in English