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-glucosyltransferase from 
Hiromoto, Takeshi; Honjo, Eijiro*; Noda, Hisanobu*; Tamada, Taro; Kazuma, Kohei*; Suzuki, Masahiko*; Blaber, M.; Kuroki, Ryota
Protein Science, 24(3), p.395 - 407, 2015/03
Times Cited Count：62 Percentile：88.99(Biochemistry & Molecular Biology)UDP-glucose: anthocyanidin 3--glucosyltransferase (UGT78K6) from catalyzes the transfer of glucose from UDP-glucose to anthocyanidins such as delphinidin. To understand the acceptor-recognition scheme of UGT78K6, the crystal structure of UGT78K6 and its complex forms with anthocyanidin delphinidin and petunidin, and flavonol kaempferol were determined to resolutions of 1.85 
, 2.55 , 2.70 and 1.75 respectively. The anthocyanidin- and flavonol-acceptor binding details are almost identical in each complex structure, although the glucosylation activities against each acceptor were significantly different. The acceptor substrates in UGT78K6 are reversely bound to its binding site by a 180
rotation about the O1-O3 axis of the flavonoid backbones observed in 
GT1 and UGT78G1. These substrate recognition schemes suggest the potential for controlled synthesis of natural pigments.
-glucosyltransferase from Hiromoto, Takeshi; Honjo, Eijiro*; Tamada, Taro; Noda, Hisanobu*; Kazuma, Kohei*; Suzuki, Masahiko*; Kuroki, Ryota
Journal of Synchrotron Radiation, 20(6), p.894 - 898, 2013/11
Times Cited Count：38 Percentile：86.45(Instruments & Instrumentation)Flowers of the butterfly pea () accumulate a group of polyacylated anthocyanins, named ternatins, in their petals. The first step in ternatin biosynthesis is the transfer of glucose from UDP-glucose to anthocyanidins such as delphinidin, a reaction catalyzed in 
by UDP-glucose:anthocyanidin 3--glucosyltransferase (
3GT-A; AB185904). To elucidate the structure-function relationship of 3GT-A, recombinant 3GT-A was expressed in 
and its tertiary structure was determined to 1.85 , resolution by using X-ray crystallography. The structure of 3GT-A shows a common folding topology, the GT-B fold, comprised of two Rossmann-like 
/
/ domains and a cleft located between the N- and C-domains containing two cavities that are used as binding sites for the donor (UDP-Glc) and acceptor substrates. By comparing the structure of 3GT-A with that of the flavonoid glycosyltransferase GT1 from red grape (
) in complex with UDP-2-deoxy-2-fluoro glucose and kaempferol, locations of the catalytic His-Asp dyad and the residues involved in recognizing UDP-2-deoxy-2-fluoro glucose were essentially identical in 3GT-A, but certain residues of GT1 involved in binding kaempferol were found to be substituted in 3GT-A. These findings are important for understanding the differentiation of acceptor-substrate recognition in these two enzymes.
Nomura, Masahiro; Schnase, A.; Shimada, Taihei; Suzuki, Hiromitsu; Tamura, Fumihiko; Yamamoto, Masanobu; Ezura, Eiji*; Omori, Chihiro*; Hara, Keigo*; Hasegawa, Katsushi*; et al.
Proceedings of 1st International Particle Accelerator Conference (IPAC '10) (Internet), p.3723 - 3725, 2010/05
We have been operating the RF cavities loaded with MA cores with a high field gradient of more than 20 kV/m since October 2007. We have been measuring the RF cavity impedance at the shutdown periods, and we detected the impedance reductions of RCS RF cavities on January and June 2009. Taking out the RF cavities from the beam line and opening them, we found that many of cores showed a buckling at the inner radius. We report the detail of condition of MA cores and the relation between the impedance reduction and core condition.
Schnase, A.; Omori, Chihiro*; Tamura, Fumihiko; Ezura, Eiji*; Hara, Keigo*; Hasegawa, Katsushi*; Nomura, Masahiro; Shimada, Taihei; Suzuki, Hiromitsu; Takagi, Akira*; et al.
Proceedings of 6th Annual Meeting of Particle Accelerator Society of Japan (CD-ROM), p.1077 - 1079, 2010/03
Shimada, Taihei; Yamamoto, Masanobu; Suzuki, Hiromitsu; Toda, Makoto*; Hasegawa, Katsushi*; Omori, Chihiro*; Tamura, Fumihiko; Schnase, A.; Hara, Keigo*; Nomura, Masahiro; et al.
Proceedings of 6th Annual Meeting of Particle Accelerator Society of Japan (CD-ROM), p.1080 - 1082, 2010/03
Few magnetic alloys cores of the RF cavities which are installed in the 3 GeV rapid cycling synchrotron of the J-PARC have buckled after the operation of about two years. We heated cores in the air and measured thermal deformation in order to get information about the buckling process. We report the results.
Ito, Takachika*; Suzuki, Shoichi*; Kanaji, Sachiko*; Shiraishi, Hiroshi*; Ota, Shoichiro*; Arima, Kazuhiko*; Tanaka, Go*; Tamada, Taro; Honjo, Eijiro*; Garcia, K. C.*; et al.
Journal of Biological Chemistry, 284(36), p.24289 - 24296, 2009/09
Times Cited Count：24 Percentile：45.24(Biochemistry & Molecular Biology)Both IL-4 and IL-13 can bind to the shared receptor composed of the IL-4 receptor chain and the IL-13 receptor -1 chain (IL-13R1); however, the assembly mechanisms of these ligands to the receptor is different, enabling the principal functions of these ligands to be different. We have previously shown that the N-terminal Ig-like domain in IL-13R1, called the D1 domain, is the specific and critical binding unit for IL-13. However, it has still remained obscure which the amino acid has specific binding capacity to IL-13 and why the D1 domain acts as the binding site for IL-13, but not IL-4. To address these questions, in this study, we performed the mutational analyses for the D1 domain, combining the structural data to identify the amino acids critical for binding to IL-13. Mutations of Lys76, Lys77, or Ile78 in c' strand in which the crystal structure showed interact with IL-13 and those of Trp65 and Ala79 adjacent to the interacting site, resulted in significant impairment of IL-13 binding, demonstrating that these amino acids generate the binding site. Furthermore, mutations of Val35, Leu38, or Val42 at N-terminal -strand also resulted in loss of IL-13 binding, probably from decrease structural stability. None of the mutations employed here affected IL-4 binding. These results demonstrate that the hydrophobic patch composed of Lys76, Lys77, and Ile78 is the IL-13 recognition site and solidify our understanding that the differential requirements of the D1 domain in IL-13R1 allows the shared receptor to respond differentially to IL-4 and IL-13.
Schnase, A.; Tamura, Fumihiko; Yamamoto, Masanobu; Nomura, Masahiro; Hasegawa, Katsushi; Shimada, Taihei; Suzuki, Hiromitsu; Omori, Chihiro*; Yoshii, Masahito*; Ezura, Eiji*; et al.
Proceedings of 2009 Particle Accelerator Conference (PAC '09) (DVD-ROM), p.1988 - 1990, 2009/05
Schnase, A.; Ezura, Eiji*; Hara, Keigo*; Hasegawa, Katsushi; Nomura, Masahiro; Omori, Chihiro*; Shimada, Taihei; Suzuki, Hiromitsu; Takagi, Akira*; Tamura, Fumihiko; et al.
Proceedings of 5th Annual Meeting of Particle Accelerator Society of Japan and 33rd Linear Accelerator Meeting in Japan (CD-ROM), p.340 - 342, 2008/00
Tamura, Fumihiko; Schnase, A.; Nomura, Masahiro; Yamamoto, Masanobu; Suzuki, Hiromitsu; Shimada, Taihei; Hasegawa, Katsushi; Yoshii, Masahito*; Omori, Chihiro*; Toda, Makoto*; et al.
Proceedings of 5th Annual Meeting of Particle Accelerator Society of Japan and 33rd Linear Accelerator Meeting in Japan (CD-ROM), p.337 - 339, 2008/00
The beam commissioning of the J-PARC RCS was started in October 2007 and acceleration of 
protons has been successfully achieved in February 2008. Also, the beam commissioning of MR began in May 2008. The RF capture of the beam and the extraction at the injection energy of 3 GeV was successfully performed. In both of the RCS and MR, MA (magnetic alloy) cavities are employed to achieve high accelerating voltages which are necessary to accelerate the high intensity proton beams. We employ full-digital LLRF control systems to realize precise and reproducible control of RF frequencies, voltages and phases. We present the status of the RF acceleration with examples of the beam signals. Also we discuss the future plans.
Nomura, Masahiro; Tamura, Fumihiko; Schnase, A.; Yamamoto, Masanobu; Hasegawa, Katsushi; Shimada, Taihei; Suzuki, Hiromitsu; Ezura, Eiji*; Hara, Keigo*; Toda, Makoto*; et al.
Proceedings of 5th Annual Meeting of Particle Accelerator Society of Japan and 33rd Linear Accelerator Meeting in Japan (CD-ROM), p.246 - 248, 2008/00
We have been developing MA core for J-PARC Ring RF Cavity. From long run tests, we found that poor electrical isolation between layers caused damages to the cores. We improved the electrical isolation mainly by introducing a smooth tension control system to the winding process. The degree of the each core's electrical isolation was evaluated by the results of DC resistance and radial RF impedance measurements. After long run tests of all of the cores, we installed 10 RF cavities in a tunnel in May 2007 and beam commissioning was started from October 2007. Until now, total operation time of the RF cavities in the tunnel is about 1500 hours. To check the condition of the cores, we measured the RF cavity impedance at intervals of beam operations. The result of impedance measurements shows that no impedance reduction related to core damage was observed.
Yamamoto, Masanobu; Anami, Shozo*; Ezura, Eiji*; Hasegawa, Katsushi; Hara, Keigo*; Horino, Mitsuyoshi*; Nomura, Masahiro; Omori, Chihiro*; Schnase, A.; Shimada, Taihei; et al.
Proceedings of 5th Annual Meeting of Particle Accelerator Society of Japan and 33rd Linear Accelerator Meeting in Japan (CD-ROM), p.358 - 360, 2008/00
The beam commissioning has been started at the J-PARC RCS in October 2007, and the beam acceleration and the extraction at 3 GeV has been succeeded. Some longitudinal bunch gymnastics has been tested toward the high intensity operation, one of the issues is arriviating the space charge effect at the injeciton. Since the multi-turn injection scheme is employed at the RCS, the electric charge density of the bunch near the center of the RF bucket tends to be high with only normal fundamental RF capture. Some schemes such as adding 2nd higher harmonic RF, the momentum offset and the phase offset of the 2nd one are simulated by longitudinal particle tracking code to make the bunching factor low. The comparison between the beam test result and the particle tracking simulation is described.
; ; ; Yokoyama, Kaoru*
Consultaints Meeting on Nuclean Fuel Czcle 
, 0 Pages, 1999/00
None
Suzuki, Eiji; Suto, Osamu; Mikami, Hisashi*
Journal of Nuclear Science and Technology, 35(6), p.425 - 436, 1998/00
Times Cited Count：1 Percentile：15.02(Nuclear Science & Technology)None
Suzuki, Eiji; Suto, Osamu
Journal of Nuclear Science and Technology, 35(11), p.788 - 795, 1998/00
Times Cited Count：0 Percentile：0.01(Nuclear Science & Technology)None
Saito, Tatsuo; Mizukaki, T.; Suzuki, Eiji; Suto, O.; Mikami, Taiji*
Proceedings of 6th Workshop on Separation phenomena in liquids and gases (SPLG '98), 298- Pages, 1998/00
None
-glucosyltransferase from Hiromoto, Takeshi; Honjo, Eijiro*; Tamada, Taro; Kuroki, Ryota; Noda, Hisanobu*; Kazuma, Kohei*; Suzuki, Masahiko*
no journal, ,
UDP-glucose: anthocyanidin 3--glucosyltransferase from (3GT-A) catalyzes the transfer of glucose from UDP-glucose to anthocyanidins such as delphinidin. The glucosylation of delphinidin at the 3-hydroxyl group has been proposed as an initial glucosylation step toward the biosynthesis of ternatins, which are blue anthocyanins found in the petals of . Although the crystal structures of several flavonoid glycosyltransferases (UGTs) were determined, the acceptor-substrate complexes were limited to the flavonol-bound forms. Here, in order to understand the acceptor-recognition scheme of 3GT-A, the crystal structures in complex with anthocyanidin delphinidin and petunidin, and flavonol kaempferol were determined to resolutions of 2.6 , 2.7 , and 1.8 respectively. The enzyme recognition of unstable anthocyanidins was firstly observed in this structural determination; nevertheless, the molecular orientations of these three acceptors in the binding site are different from those of the known flavonoid UGTs, GT1 and UGT78G1. The crystal structures of 3GT-A provide insight not only into anthocyanidin configurations in enzyme, but also into a different binding scheme for acceptor-substrate recognition compared with the known UGTs.
