Genetic survey of indigenous microbial eukaryotic communities, mainly fungi, in sedimentary rock matrices of deep terrestrial subsurface

Saito, Yoshimoto*; Hirano, Shinichi*; Nagaoka, Toru*; Amano, Yuki

Ecological Genetics and Genomics, 12, p.100042_1 - 100042_9, 2019/10

https://doi.org/10.1016/j.egg.2019.100042

Culture-independent molecular techniques enable us to analyze microflora in various environments. Many uncultured prokaryotes have been detected by the molecular methods from extreme environments, including anaerobic, no light, high-pressure, and high temperature. Recently, microbial eukaryotes were also detected in deep-sea environments, suggesting that microbial eukaryotes can adapt to a wider range of environments than previously thought. Here, we performed a culture-independent analysis of eukaryotes from approximately -250 m depth in the Horonobe Underground Research Laboratory at Horonobe, Japan. Our results indicate that fungi are the dominant eukaryotic flora in deep sedimentary rocks of Horonobe. We detected a wide range of species, including Zygomycete, Basidiomycete, and Ascomycete fungi from the rocks. This study is the first report of eukaryotic diversity in deep subsurface sedimentary rocks.

A Study to control chemical reactions using Si:2p core ionization; Site-specific fragmentation

Nagaoka, Shinichi*; Fukuzawa, Hironobu*; Prmper, G.*; Takemoto, Mai*; Takahashi, Osamu*; Yamaguchi, Takuhiro*; Kakiuchi, Takuhiro*; Tabayashi, Kiyohiko*; Suzuki, Isao*; Harries, J.; et al.

Journal of Physical Chemistry A, 115(32), p.8822 - 8831, 2011/07

https://doi.org/10.1021/jp203664r

Hafnium adsorption on clean Si(110)162 single domain surface studied by low energy electron diffraction and electron spectroscopy

Kakiuchi, Takuhiro*; Katsuragi, Takuma*; Nakano, Yuji*; Yoshigoe, Akitaka; Nagaoka, Shinichi*; Mase, Kazuhiko*

no journal, , 

HfO has been becoming important as a future dielectric material for future metal-oxide-semiconductor devices. In this study, surface structure, surface chemical state, and valence electronic structure after oxidation of single domain Si(110)-162 surface have precisely measured by using low-energy-electron diffraction and synchrotron radiation photroemission spectroscopy. It was found that five possible adsorption sites (SC1-SC5) of the single domain Si(110)-162 surface are uniformely reacted with Hf atoms with increasing its exposure, indicating that random Hf adsorption takes place on the surface.

Bio-corrosion of carbon steel in compacted buffer materials

Nagaoka, Toru*; Hirano, Shinichi*; Matsumoto, Norio*; Amano, Yuki

no journal, , 

no abstracts in English

Laboratory and in-situ examinations of microbial abundance, composition and activity in compacted bentonite buffer materials

Amano, Yuki; Beppu, Hikari*; Ono, Hirokazu; Taniguchi, Naoki; Hirano, Shinichi*; Matsumoto, Norio*; Nagaoka, Toru*

no journal, , 

Microbial influenced corrosion of carbon steel in compacted buffer material

Hirano, Shinichi*; Nagaoka, Toru*; Matsumoto, Norio*; Amano, Yuki

no journal, , 

no abstracts in English