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Journal Articles

"Live-autoradiography" technique reveals genetic variation in the rate of Fe uptake by barley cultivars

Higuchi, Kyoko*; Kurita, Keisuke; Sakai, Takuro; Suzui, Nobuo*; Sasaki, Minori*; Katori, Maya*; Wakabayashi, Yuna*; Majima, Yuta*; Saito, Akihiro*; Oyama, Takuji*; et al.

Plants (Internet), 11(6), p.817_1 - 817_11, 2022/03

 Times Cited Count:2 Percentile:34.8(Plant Sciences)

Genetic diversity in the rate of Fe uptake by plants has not been broadly surveyed among plant species or genotypes, although plants have developed various Fe acquisition mechanisms. We adopted the "Live-autoradiography" technique with radioactive $$^{59}$$Fe to directly evaluate the uptake rate of Fe by barley cultivars from a nutrient solution containing a very low concentration of Fe. Our observations revealed that the ability to acquire Fe from the low Fe solution was not always the sole determinant of tolerance to Fe deficiency among the barley genotypes.

Oral presentation

The Relationship between Fe influx into new leaf and downregulation of SUF machinery in Fe-deficient barley

Higuchi, Kyoko*; Sasaki, Minori*; Katori, Maya*; Kurita, Keisuke; Sakai, Takuro; Suzui, Nobuo*; Kawachi, Naoki*; Saito, Akihiro*; Oyama, Takuji*

no journal, , 

Expression of genes involved in the Sulfur mobilization (SUF) machinery which assembles and inserts Fe-S clusters into client proteins in chloroplast is downregulated by depletion of Fe as a substrate. The pattern and timing of their downregulation are different among barley cultivars, and that difference seems to be related to the maintenance of Photosystem I under Fe-deficient condition. These characters of barley cultivars are, however, not always correlated with overall tolerance to Fe deficiency nor total Fe concentration of leaves. Thus, we investigated the relationship between Fe influx into new leaf and downregulation of SUF machinery in Fe-deficient barley.

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