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condensed state in 
Mg using the 
C+C scatteringFujikawa, Y.*; Kawabata, T.*; Adachi, S.*; Hirose, Kentaro; Makii, Hiroyuki; Nishio, Katsuhisa; Orlandi, R.; Suzaki, Fumi; 13 of others*
Physics Letters B, 848, p.133834_1 - 133834_6, 2024/01
Times Cited Count：0 Percentile：0.02(Astronomy & Astrophysics)
Tamii, Atsushi*; Pellegri, L.*; S
derstrm, P.-A.*; Allard, D.*; Goriely, S.*; Inakura, Tsunenori*; Khan, E.*; Kido, Eiji*; Kimura, Masaaki*; Litvinova, E.*; et al.
European Physical Journal A, 59(9), p.208_1 - 208_21, 2023/09
Times Cited Count：1 Percentile：0.02(Physics, Nuclear)no abstracts in English
Tang, P.*; Kita, Kazuyuki*; Igarashi, Yasuhito*; Satou, Yukihiko; Hatanaka, Kotaro*; Adachi, Koji*; Kinase, Takeshi*; Ninomiya, Kazuhiko*; Shinohara, Atsushi*
Progress in Earth and Planetary Science (Internet), 9(1), p.17_1 - 17_15, 2022/03
Times Cited Count：5 Percentile：69.58(Geosciences, Multidisciplinary)
Chiera, N. M.*; Sato, Tetsuya; Eichler, R.*; Tomitsuka, Tomohiro; Asai, Masato; Adachi, Sadia*; Dressler, R.*; Hirose, Kentaro; Inoue, Hiroki*; Ito, Yuta; et al.
Angewandte Chemie; International Edition, 60(33), p.17871 - 17874, 2021/08
Times Cited Count：2 Percentile：14.88(Chemistry, Multidisciplinary)The formation and the chemical characterization of single atoms of dubnium (Db, element 105), in the form of its volatile oxychloride, was investigated using the on-line gas phase chromatography technique, in the temperature range 350 - 600 
C. Under the exact same chemical conditions, comparative studies with the lighter homologs of group-5 in the Periodic Table clearly indicate the volatility sequence being NbOCl
TaOCl
DbOCl. From the obtained experimental results, thermochemical data for DbOCl were derived. The present study delivers reliable experimental information for theoretical calculations on the chemical properties of transactinides.
Suzuki, Shintaro*; Takubo, Ko*; Kuga, Kentaro*; Higemoto, Wataru; Ito, Takashi; Tomita, Takahiro*; Shimura, Yasumichi*; Matsumoto, Yosuke*; Bareille, C.*; Wadachi, Hiroki*; et al.
Physical Review Research (Internet), 3(2), p.023140_1 - 023140_12, 2021/05
We report our experimental discovery of the transition temperature reaching 20 K in a Yb-based compound at ambient pressure. The Mn substitution at the Al site in an intermediate valence state of -YbAlB
not only induces antiferromagnetic transition at a record high temperature of 20 K but also transforms the heavy-fermion liquid state in -YbAlB into a highly resistive metallic state proximate to a Kondo insulator.
Cs to web-building spiders, 
, and its pathways; A Preliminary study using stable carbon and nitrogen isotope analysesTanaka, Sota; Kakinuma, Hotaru*; Adachi, Taro*; Atarashi-Andoh, Mariko; Koarashi, Jun
Journal of Nuclear Science and Technology, 58(4), p.507 - 514, 2021/04
Times Cited Count：0 Percentile：0.01(Nuclear Science & Technology)Understanding the pathways of Cs transfer to predatory spiders is useful to assess long-term behavior of Cs in the environment, because spiders obtain food resources from both the grazing and detritus food chains in terrestrial-aquatic linking forest ecosystems. In the present study, we collected spider samples at forest interior and riverside. The sample collection was approximately 6.5 years after the FDNPP accident. Moreover, a transfer factor value (T
) for Cs in spiders was quantified. Stable carbon and nitrogen isotope ratios (
C and 
N) of the spiders were also investigated to estimate the transfer pathways of Cs to the spiders.
Miyazaki, Yasunori; Adachi, Junichi*; Masuda, Ryotaro*; Gejo, Tatsuo*; Hoshino, Masamitsu*
Photon Factory Activity Report 2021 (Internet), 2 Pages, 2021/00
no abstracts in English
-olefin using small-angle X-ray scatteringOba, Yojiro; Motokawa, Ryuhei; Hino, Masahiro*; Adachi, Nozomu*; Todaka, Yoshikazu*; Inoue, Rintaro*; Sugiyama, Masaaki*
Chemistry Letters, 49(7), p.823 - 825, 2020/07
Times Cited Count：0 Percentile：0(Chemistry, Multidisciplinary)Tanaka, Sota; Adachi, Taro*; Takahashi, Tomoyuki*; Takahashi, Sentaro*
Low-Dose Radiation Effects on Animals and Ecosystems; Long-Term Study on the Fukushima Nuclear Accident, p.43 - 52, 2020/00
Times Cited Count：1 Percentile：12.16(Ecology)To understand the behavior of radiocesium in terrestrial invertebrates, chronological changes in the concentration of radiocesium in arthropods and earthworms from different trophic levels were investigated after the Fukushima Daiichi Nuclear Power Plant accident. The median radiocesium concentrations in the rice grasshopper, Oxya yezoensis, and the Emma field cricket, Teleogryllus emma, were 0.46 and 0.15 Bq/g fw in 2012, respectively, which dropped continuously to 0.05 and 0.01 Bq/g fw in 2016. In contrast, no significant reduction in radiocesium concentration was observed in the Joro Spider, Nephila clavata, in which the concentration was 0.31 Bq/g fw in 2012 and remained at 0.14 Bq/g fw in 2016. The comparison of radiocesium concentrations at each trophic level showed that the amount in detritivorous earthworms was 85 times higher than in herbivorous grasshoppers. This suggests that detritus food web could be a primary pathway of radiocesium through food webs.
Tanaka, Sota; Kakinuma, Hotaru*; Adachi, Taro*; Takahashi, Tomoyuki*; Takahashi, Sentaro*
KEK Proceedings 2019-2, p.179 - 182, 2019/11
The concentration of Cs in flying insects was investigated after the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident. The insects were collected at about 11 km north west of the FDNPP at two different sites in the forest and riverside. The median concentration of Cs in flying insects in the forest was significantly higher than riverside. However, high concentration of Cs in detritus fly (Diptera: Calliphoridae) was observed at both sites. The concentration of fly at the forest and riverside were 16
10
Bq/kg fw and 1810 Bq/kg fw, respectively. The result shows that the concentration of radiocesium in detritus fly were still high 8 years after the accident. This suggests that detritus food chain is a dominant transfer pathway of radiocesium to higher trophic level species such as spiders.
Oba, Yojiro*; Morooka, Satoshi; Oishi, Kazuki*; Sato, Nobuhiro*; Inoue, Rintaro*; Adachi, Nozomu*; Suzuki, Junichi*; Tsuchiyama, Toshihiro*; Gilbert, E. P.*; Sugiyama, Masaaki*
Journal of Applied Crystallography, 49(5), p.1659 - 1664, 2016/10
Times Cited Count：13 Percentile：66.73(Chemistry, Multidisciplinary)
binding site of a 
-lactamase from the halophile by anomalous X-ray diffractionArai, Shigeki; Shibazaki, Chie; Shimizu, Rumi; Adachi, Motoyasu; Tamada, Taro; Tokunaga, Hiroko*; Ishibashi, Matsujiro*; Tokunaga, Masao*; Kuroki, Ryota
Kyushu Shinkurotoronko Kenkyu Senta Nempo, 2014, p.17 - 19, 2016/03
no abstracts in English
-lactamase from the moderate halophile 
sp.560 and the discovery of a Cs-selective binding siteArai, Shigeki; Yonezawa, Yasushi*; Okazaki, Nobuo*; Matsumoto, Fumiko*; Shibazaki, Chie; Shimizu, Rumi; Yamada, Mitsugu*; Adachi, Motoyasu; Tamada, Taro; Kawamoto, Masahide*; et al.
Acta Crystallographica Section D, 71(3), p.541 - 554, 2015/03
Times Cited Count：7 Percentile：50.76(Biochemical Research Methods)The crystal structure of halophilic -lactamase from sp.560 (HaBLA) was determined using X-ray crystallography. Moreover, the locations of bound Sr
and Cs ions were identified by anomalous X-ray diffraction. The location of one Cs specific binding site was identified on HaBLA even in the presence of 9-fold molar excess of Na (90 mM Na /10 mM Cs). This Cs binding site is formed by two main-chain O atoms and an aromatic ring of a side chain of Trp. An aromatic ring of Trp interacts with Cs by the cation-
interaction. The observation of a selective and high-affinity Cs binding site provides important information that is useful for designing artificial Cs binding sites useful in bioremediation of radioactive isotopes.
sp.593Arai, Shigeki; Yonezawa, Yasushi*; Ishibashi, Matsujiro*; Matsumoto, Fumiko*; Adachi, Motoyasu; Tamada, Taro; Tokunaga, Hiroko*; Blaber, M.; Tokunaga, Masao*; Kuroki, Ryota
Acta Crystallographica Section D, 70(3), p.811 - 820, 2014/03
Times Cited Count：12 Percentile：63.07(Biochemical Research Methods)In order to clarify the structural basis of halophilic characteristics of an alkaline phosphatase derived from the moderate halophile sp.593 (HaAP), the tertiary structure of HaAP was determined to 2.1
resolution by X-ray crystallography. Structural properties of surface negative charge and core hydrophobicity are shown to be intermediate between halophile and non-halophile characteristics, and may explain the unique functional adaptation to a wide-range of salt concentration.
Tamada, Taro; Adachi, Motoyasu; Kurihara, Kazuo; Kuroki, Ryota
Nihon Kessho Gakkai-Shi, 55(1), p.47 - 51, 2013/02
Neutron crystallography enables us to identify the accurate hydrogen positions in proteins, which play important roles in many chemical reactions in living system. Here we show our results of neutron structure determination of enzymes in complex with its inhibitors which corresponds to transition state analogues. Neutron structure analysis elucidated the detail catalytic reaction of each enzyme by direct observation of hydrogen atoms. Furthermore we would like to introduce a new neutron beam line for neutron structural biology planned at MLF in J-PARC.
Nakaniwa, Tetsuko*; Fukata, Harumi*; Inoue, Tatsuya*; Goda, Masaki*; Nakai, Ryoko*; Kirii, Yasuyuki*; Adachi, Motoyasu; Tamada, Taro; Segawa, Shinichi*; Kuroki, Ryota; et al.
Biochemistry, 51(42), p.8410 - 8421, 2012/10
Times Cited Count：18 Percentile：42.99(Biochemistry & Molecular Biology)Protein kinase is a vital drug target for the treatment of a wide range of diseases. To investigate the effect of cysteine mutation on the function, stability and structure of kinase, free cysteines of c-Jun N-terminal kinase 1 (JNK1) were systematically removed by mutation. Two cysteine-destructed mutants in which three (M3) and seven (M7) cysteine residues are removed, yielded about 5 and 2 times than wild type JNK-1 (M0). SDS PAGE analysis showed that the aggregation was less in the case of M3 and M7. Thermal unfolding experiment of M0, M3 and M7 using by differential scanning calorimetry proceeded at least three state unfolding. Crystal structure of the M3 mutant was determined to 2.6 resolution, which was identical to that of the wild-type. Consequently, due to the highest yield, its improved stability against aggregation and its structural similarity to the wild type, the M3 mutant is suitable for the use of further characterization of its function and structure.
HB8Okazaki, Nobuo; Adachi, Motoyasu; Tamada, Taro; Kurihara, Kazuo; Oga, Takushi*; Kamiya, Nobuo*; Kuramitsu, Seiki*; Kuroki, Ryota
Acta Crystallographica Section F, 68(1), p.49 - 52, 2012/01
Times Cited Count：2 Percentile：32.79(Biochemical Research Methods)Kuroki, Ryota; Okazaki, Nobuo; Adachi, Motoyasu; Ohara, Takashi; Kurihara, Kazuo; Tamada, Taro
Acta Crystallographica Section D, 66(11), p.1126 - 1130, 2010/11
Times Cited Count：2 Percentile：30.11(Biochemical Research Methods)It is generally known that enzymes represent important drug-target proteins. Elucidation of the catalytic function and the molecular-recognition mechanisms of enzymes provides important information for structure-based drug design. Neutron crystallography provides accurate information on the locations of H atoms that are essential in enzymatic function and molecular recognition. Recent examples are described of the structure determination of the drug-target proteins human immunodeficiency virus protease and porcine pancreatic elastase in complex with transition-state analogue inhibitors using the neutron diffractometers for biological crystallography (BIX-3 and BIX-4) installed at the JRR-3 research reactor.
Kakinouchi, Keisuke*; Nakamura, Tsutomu*; Tamada, Taro; Adachi, Hiroaki*; Sugiyama, Shigeru*; Maruyama, Mihoko*; Takahashi, Yoshinori*; Takano, Kazufumi*; Murakami, Satoshi*; Inoue, Tsuyoshi*; et al.
Journal of Applied Crystallography, 43(4), p.937 - 939, 2010/08
Times Cited Count：4 Percentile：48.31(Chemistry, Multidisciplinary)A method for growing large protein crystals is described. In this method, a cut pipette tip is used to hang large-scale droplets (maximum volume 200 
l) consisting of protein and precipitating agents. A crystal grows at the vapor-liquid interface; thereafter the grown crystal can be retrieved by droplet-droplet contact both for repeated macroseeding and for mounting crystals in a capillary. Crystallization experiments with peroxiredoxin of 
K1(thioredoxin peroxidase, ApTPx) and hen egg white lysozyme demonstrated that this large-scale hanging-drop method could produce a large-volume crystal very effectively. A neutron diffraction experiment confirmed that an ApTPx crystal (6.2 mm
) obtained by this method diffracted to beyond 3.5 resolution.
resolutionShimizu, Noriko*; Sugiyama, Shigeru*; Maruyama, Mihoko*; Takahashi, Yoshinori*; Adachi, Motoyasu; Tamada, Taro; Hidaka, Koshi*; Hayashi, Yoshio*; Kimura, Toru*; Kiso, Yoshiaki*; et al.
Crystal Growth & Design, 10(7), p.2990 - 2994, 2010/06
Times Cited Count：11 Percentile：72.02(Chemistry, Multidisciplinary)We report crystal growth of human immunodeficiency virus 1 protease (HIV PR) in a complex with its inhibitor KNI-272 by six different methods. Comparative analysis indicates that top-seeded solution growth (TSSG) and TSSG combined with the floating and stirring technique (TSSG-FAST) are efficient strategies for rapidly obtaining large single crystals and effectively preventing polycrystallization of the seed crystal. Neutron diffraction analysis confirmed that the crystalobtained by TSSG is a high-quality single crystal. Furthermore, crystal shape was observed to be influenced by solution flow, suggesting that the degree of supersaturation significantly affects the crystal growth direction of HIV PR complex. This finding implies that the shape of the HIV PR complex crystal might be controlled by the solution flow rate.
