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Journal Articles

Medaka blastoderm cells are capable of compensating the injured cells irradiated by carbon-ion micro-beam

Yasuda, Takako*; Oda, Shoji*; Asaka, Tomomi*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Yasuhiko; Mitani, Hiroshi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 85, 2015/03

In this present study, we examined the effects of heavy carbon-ions on development in pre-implantation period utilizing medaka blastula stage embryos (st. 11: blastderm diameter is about 500 $$mu$$m). We performed targeted irradiation by carbon-ion micro-beam (diameters of 120, 180 $$mu$$m) to a central parts of blastoderm and observed the abnormalities during development compared with whole-body irradiated embryos. As a results, retardation and characteristic malformed eyes were observed during development when blastoderm cells were partially irradiated, However, more than half of 50 Gy-irradiated embryos (area size=120 $$mu$$m diameter) could hatch normally in contrast to all embryos with 2 Gy of whole-body irradiation being lethal before hutching.

JAEA Reports

2nd power-up test for JRR-2

Kambara, Toyozo; Uno, Hidero; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Kohayakawa, Toru; Takayanagi, Hiroshi; Fujimura, Tsutomu; Morita, Morito; Ichihara, Masahiro; et al.

JAERI 1045, 11 Pages, 1963/03

JAERI-1045.pdf:0.72MB

no abstracts in English

JAEA Reports

The First loading fuel elements and power-up for JRR-2

JRR-2 Control Office; Kambara, Toyozo; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Kohayakawa, Toru; Morozumi, Minoru; Kambayashi, Yuichiro; Shitomi, Hajimu; Kokanezawa, Takashi; et al.

JAERI 1027, 57 Pages, 1962/09

JAERI-1027.pdf:4.76MB

no abstracts in English

JAEA Reports

Treatment and analysis of the water and gas in JRR-2

JRR-2 Operations Office; Kambara, Toyozo; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Haginoya, Kinichi; Kohayakawa, Toru; Yamaki, Jikei; Yokota, Mitsuo; Horiki, Oichiro; et al.

JAERI 1024, 79 Pages, 1962/08

JAERI-1024.pdf:5.66MB

no abstracts in English

JAEA Reports

Critical experiments and characteristic measurement for JRR-2

JRR-2 Critical Experiments Group; Kambara, Toyozo; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Kohayakawa, Toru; Morozumi, Minoru; Kambayashi, Yuichiro; Shitomi, Hajimu; Kokanezawa, Takashi; et al.

JAERI 1025, 62 Pages, 1962/03

JAERI-1025.pdf:4.6MB

no abstracts in English

Oral presentation

Target irradiation of the testis of Medaka fish using carbon-ion microbeam

Oda, Shoji*; Yasuda, Takako*; Asaka, Tomomi*; Mitani, Hiroshi*; Ikeda, Hiroko; Muto, Yasuko*; Yokota, Yuichiro; Suzuki, Michiyo; Funayama, Tomoo; Kobayashi, Yasuhiko

no journal, , 

The method for targeting and irradiating the testis of fry and adult of Medaka fish using carbon-ion microbeam was established. To irradiate the testis of the fry, a transgenic Medaka that expresses GFP protein specifically in its testis was used. The position of the testis was observed under fluorescent microscopy and then the testis was targeted and irradiated with carbon ion microbeam. To irradiate the testis of the adult Medaka, a adult male of p53 knock out transgenic fish was used. The microbeam was irradiated on the estimated testis position; thereafter the success of irradiation was confirmed by the induction of testis-ova, which is known as induced by the radiation of testis in p53 knock out fish.

Oral presentation

3D analysis by whole body serial sections of the systemic responses induced by local carbon-ion irradiation in medaka (${it Oryzias latipes}$)

Asaka, Tomomi*; Hashimoto, Chika*; Ito, Kazusa*; Yasuda, Takako*; Nagata, Kento*; Nishimaki, Toshiyuki*; Katsumura, Takafumi*; Ota, Hiroki*; Suzuki, Michiyo; Funayama, Tomoo; et al.

no journal, , 

It is thought that the irradiation to the particular area of the individual could induce the irradiation impact to the outside of the irradiated area in addition to the irradiated region. In this study, we established the procedures to conduct carbon ion irradiation on adult Medaka, which can irradiate only either dorsal or ventral side of the fish, using the broad-beam irradiation system of JAEA-Takasaki. The irradiated fish were examined heart rate before the fixation. Thereafter, the systemic alteration was evaluated by serial cross sections of the whole body of the fish. Tissues at the irradiated side were seriously injured that corresponded to the range of the beam. However, secondary gill lamella showed the characteristic edema in either the ventral or dorsal irradiated fish unless the gill located at ventral, suggesting the systemic irradiation effect. There was no alteration in the heart rate after the irradiation suggesting no severe effects in blood flow in this condition.

Oral presentation

The Systemic effects of irradiation revealed by the local irradiation to the medaka testis using carbon-ion microbeam system in TIARA

Oda, Shoji*; Yasuda, Takako*; Hibi, Yusuke*; Asaka, Tomomi*; Ikeda, Hiroko; Muto, Yasuko*; Yokota, Yuichiro; Sakashita, Tetsuya; Suzuki, Michiyo; Funayama, Tomoo; et al.

no journal, , 

We have developed a new irradiation protocol for tissue specific irradiation to living embryo and adult medaka, ${it Oryzias latipes}$. In the study, a testis of p53 knockout fish were irradiated with multi-shots of microbeam. Less numbers of testis-ova were induced in the in p53- lacking testis 1 week after the irradiation compared to whole body irradiated testis. Specific irradiation of microbeam onto the right (or left) lobe of the optic tectum in medaka embryos of 2 days post fertilization ectopically induced apoptotic cells in the body trunk and the tail in addition to the irradiated position. The systemic effects as endocrine system, autonomic nervous system and immunity reactions occur in adult medaka and these results suggested that the systemic effects might be functional even in developing embryos.

Oral presentation

Availability of medaka as systemic effect model of local irradiation by histological analysis

Asaka, Tomomi*; Ito, Kazusa*; Hashimoto, Chika*; Yasuda, Takako*; Nagata, Kento*; Nishimaki, Toshiyuki*; Katsumura, Takafumi*; Ota, Hiroki*; Suzuki, Michiyo; Funayama, Tomoo; et al.

no journal, , 

Radiotherapy has been widely used in cancer target. It is thought that the irradiation to the particular area of the individual could induce the irradiation impact to the nearby and distant tissues of the irradiated area in addition to the irradiated region. However, it is still difficult to analyze influences to the whole body using mice because of its big body size. Medaka, ${it Oryzias latipes}$, is small enough to examine the acute somatic effects induced in all tissues at the cellular levels. In this study, we developed a method to conduct local irradiation on adult medaka using low energy carbon-ion beam in TIARA, JAEA-Takasaki. The irradiated fish were measured the heart rate to evaluate the impact to the heart, chemically fixed, serially sectioned and Hematoxylin/eosin stained. The results suggested that there would be the systemic effects by local irradiation in addition to the effects in the irradiated region, which is probably caused by immune system though blood.

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