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Journal Articles

Effect of ionizing radiation upon dehydrated Pv11 cultured cells originated from the sleeping chironomid

Watanabe, Kazuyo*; Akitsuki, Takashi*; Shimura, Sachiko*; Gusev, O.*; Cornette, R.*; Kikawada, Takahiro*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko; Okuda, Takashi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 87, 2015/03

The Sleeping Chironomid, ${it Polypedilum vanderplanki}$ can stand complete desiccation (anhydrobiosis) and also shows radio-resistance. Recently, we have generated cultured cell (Pv11) originated from ${it P. vanderplanki}$ embryo which can also stand complete dehydration. In this study, we examine the tolerance of cultured cell Pv11 against ionbeam irradiation.

Journal Articles

Anhydrobiosis-associated nuclear DNA damage and repair in the Sleeping Chironomid; Linkage with radioresistance

Gusev, O.*; Nakahara, Yuichi*; Vanyagina, V.*; Malutina, L.*; Cornette, R.*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Kikawada, Takahiro*; Kobayashi, Yasuhiko; Okuda, Takashi*

PLoS ONE (Internet), 5(11), p.e14008_1 - e14008_9, 2010/11

 Times Cited Count:49 Percentile:75.31(Multidisciplinary Sciences)

Anhydrobiotic chironomid larvae can withstand prolonged complete desiccation as well as other external stresses including ionizing radiation. To understand the cross-tolerance mechanism, we have analyzed the damage and repair in the nuclear DNA using DNA comet assays and gene expression in relation to anhydrobiosis and radiation. We found that dehydration causes alterations in chromatin structure and a severe fragmentation of nuclear DNA in the cells of the larvae despite successful anhydrobiosis. Furthermore, while the larvae have restored physiological activity within an hour following rehydration, nuclear DNA restoration typically took 72 to 96 h.

Oral presentation

Radiation tolerance and anhydrobiosis in African chironomid, ${it Polypedilum vanderplanki}$

Nakahara, Yuichi*; Watanabe, Masahiko*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Gusev, O.*; Fujita, Akihiko*; Kikawada, Takahiro*; Horikawa, Daiki*; Kobayashi, Yasuhiko; Okuda, Takashi*

no journal, , 

The present study aims to evaluate effects of anhydrobiosis on radiation tolerance in an anhydrobiotic insect, ${it Polypedilum vanderplanki}$. Wet larvae were killed in a dose-dependent manner at doses higher than 2000 Gy, and all died within 8 h after 4000 Gy exposure. However, anhydrobiosis did not protect against radiation damage in terms of producing viable offspring. These results indicate that anhydrobiosis enhances radiotolerance in larvae, but not in the delayed effects of development.

Oral presentation

Spore of Funaria hygrometrica (moss) which exposed on the outer wall of the Pirs docking node of ISS for 13 months germinated

Takahashi, Yuichi*; Shibata, Shimpei*; Yokoyama, Jun*; Hashimoto, Hirofumi*; Yokobori, Shinichi*; Yamagishi, Akihiko*; Kawaguchi, Yuko*; Gusev, O.*; Narumi, Issei; Sato, Katsuya; et al.

no journal, , 

no abstracts in English

Oral presentation

16S rRNA gene amplicon sequencing analysis of microorganisms derived from environmental samples near the Fukushima Daiichi Nuclear Power Station

Warashina, Tomoro*; Sato, Asako*; Shagimardanova, E.*; Maruyama, Shigenori*; Mori, Hiroshi*; Narumi, Issey*; Gusev, O.*; Saito, Motofumi*; Sanada, Yukihisa; Sasaki, Yoshito; et al.

no journal, , 

no abstracts in English

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