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Journal Articles

Status of the neutron time-of-flight single-crystal diffraction data-processing software STARGazer

Yano, Naomine*; Yamada, Taro*; Hosoya, Takaaki*; Ohara, Takashi; Tanaka, Ichiro*; Niimura, Nobuo*; Kusaka, Katsuhiro*

Acta Crystallographica Section D; Structural Biology (Internet), 74(11), p.1041 - 1052, 2018/11

 Times Cited Count:11 Percentile:74.45(Biochemical Research Methods)

Journal Articles

Structure analysis and derivation of deformed electron density distribution of polydiacetylene giant single crystal by the combination of X-ray and neutron diffraction data

Tashiro, Koji*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; Ohara, Takashi; Hanesaka, Makoto*; Yoshizawa, Yoshinori*; Yamamoto, Hiroko*; Niimura, Nobuo*; Tanaka, Ichiro*; Kurihara, Kazuo*; et al.

Macromolecules, 51(11), p.3911 - 3922, 2018/06

 Times Cited Count:4 Percentile:14.27(Polymer Science)

Journal Articles

Application of profile fitting method to neutron time-of-flight protein single crystal diffraction data collected at the iBIX

Yano, Naomine*; Yamada, Taro*; Hosoya, Takaaki*; Ohara, Takashi; Tanaka, Ichiro*; Kusaka, Katsuhiro*

Scientific Reports (Internet), 6, p.36628_1 - 36628_9, 2016/12

 Times Cited Count:11 Percentile:61.85(Multidisciplinary Sciences)

Journal Articles

Accurate structure analyses of polymer crystals on the basis of wide-angle X-ray and neutron diffractions

Tashiro, Koji*; Hanesaka, Makoto*; Yamamoto, Hiroko*; Wasanasuk, K.*; Jayaratri, P.*; Yoshizawa, Yoshinori*; Tanaka, Ichiro*; Niimura, Nobuo*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; et al.

Kobunshi Rombunshu, 71(11), p.508 - 526, 2014/11

 Times Cited Count:6 Percentile:22.47(Polymer Science)

The crystal structure analysis of various polymer substances has been reviewed on the basis of wide-angle high-energy X-ray and neutron diffraction data. The progress in structural analytical techniques of polymer crystals have been reviewed at first. The structural models proposed so far were reinvestigated and new models have been proposed for various kinds of polymer crystals including polyethylene, poly(vinyl alcohol), poly(lactic acid) and its stereocomplex etc. The hydrogen atomic positions were also clarified by the quantitative analysis of wide-angle neutron diffraction data, from which the physical properties of polymer crystals have been evaluated theoretically. The bonded electron density distribution has been estimated for a polydiacetylene single crystal on the basis of the so-called X-N method or by the combination of structural information derived from X-ray and neutron diffraction data analysis. Some comments have been added about future developments in the field of structure-property relationship determination.

Journal Articles

Detector system of the $$SENJU$$ single-crystal time-of-flight neutron diffractometer at J-PARC/MLF

Kawasaki, Takuro; Nakamura, Tatsuya; To, Kentaro; Hosoya, Takaaki*; Oikawa, Kenichi; Ohara, Takashi; Kiyanagi, Ryoji; Ebine, Masumi; Birumachi, Atsushi; Sakasai, Kaoru; et al.

Nuclear Instruments and Methods in Physics Research A, 735, p.444 - 451, 2014/01

 Times Cited Count:18 Percentile:80.26(Instruments & Instrumentation)

Journal Articles

Evaluation of performance for IBARAKI biological crystal diffractometer iBIX with new detectors

Kusaka, Katsuhiro*; Hosoya, Takaaki*; Yamada, Taro*; Tomoyori, Katsuaki; Ohara, Takashi; Katagiri, Masaki*; Kurihara, Kazuo; Tanaka, Ichiro*; Niimura, Nobuo*

Journal of Synchrotron Radiation, 20(6), p.994 - 998, 2013/11

 Times Cited Count:35 Percentile:86.09(Instruments & Instrumentation)

The IBARAKI biological crystal diffractometer, iBIX, is a high-performance time-of-flight neutron single-crystal diffractometer for elucidating mainly the hydrogen, protonation and hydration structures of biological macromolecules in various life processes. Since the end of 2008, iBIX has been available to user's experiments supported by Ibaraki University. Since August 2012, an upgrade of the 14-existing detectors has begun and 16 new detectors have been installed for iBIX. The total measurement efficiency of the present diffractometer has been impoved by one order of magnitude from the previous one with the increasing of accelerator power. In December 2012, commissioning of the new detectors was successful, and collection of the diffraction dataset of ribonucrease A as a standard protein was attempted in order to estimate the performance of the upgraded iBIX in comparison with previous results. The resolution of diffraction data, equivalence among intensities of symmetry-related reflections and reliability of the refined structure have been improved dramatically. iBIX is expected to be one of the highest-performance neutron single-crystal diffractometers for biological macromolecules in the world.

Journal Articles

Hydrogen-bond network and pH sensitivity in human transthyretin

Yokoyama, Takeshi*; Mizuguchi, Mineyuki*; Nabeshima, Yuko*; Kusaka, Katsuhiro*; Yamada, Taro*; Hosoya, Takaaki*; Ohara, Takashi; Kurihara, Kazuo; Tanaka, Ichiro*; Niimura, Nobuo*

Journal of Synchrotron Radiation, 20(6), p.834 - 837, 2013/11

 Times Cited Count:6 Percentile:33.75(Instruments & Instrumentation)

Transthyretin (TTR) is a tetrameric protein. TTR misfolding and aggregation are associated with human amyloid diseases. Dissociation of the TTR tetramer is believed to be the rate-limiting step in the amyloid fibril formation cascade. Low pH is known to promote dissociation into monomer and the formation of amyloid fibrils. In order to reveal the molecular mechanisms underlying pH sensitivity and structural stabilities of TTR, neutron diffraction studies were conducted using the IBARAKI Biological Crystal Diffractometer with the time-of-flight method. Crystals for the neutron diffraction experiments were grown up to 2.5 mm$$^{3}$$ for four months. The neutron crystal structure solved at 2.0 ${AA}$ revealed the protonation states of His88 and the detailed hydrogen-bond network depending on the protonation states of His88. This hydrogen-bond network is involved in monomer-monomer and dimer-dimer interactions, suggesting that the double protonation of His88 by acidification breaks the hydrogen-bond network and causes the destabilization of the TTR tetramer. Structural comparison with the X-ray crystal structure at acidic pH identified the three amino acid residues responsible for the pH sensitivity of TTR. Our neutron model provides insights into the molecular stability related to amyloidosis.

Journal Articles

Structure of morpholinium tribromoplumbate C$$_{4}$$H$$_{8}$$ONH$$_{2}$$PbBr$$_{3}$$ studied using single-crystal neutron diffraction

Kawasaki, Takuro; Takahashi, Miwako*; Ohara, Takashi*; Tanaka, Ichiro*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; Yamada, Taro*; Kurihara, Kazuo

Journal of the Physical Society of Japan, 81(9), p.094602_1 - 094602_6, 2012/09

 Times Cited Count:7 Percentile:46.8(Physics, Multidisciplinary)

Journal Articles

Hydrogen-bond network and pH sensitivity in transthyretin; Neutron crystal structure of human transthyretin

Yokoyama, Takeshi*; Mizuguchi, Mineyuki*; Nabeshima, Yuko*; Kusaka, Katsuhiro*; Yamada, Taro*; Hosoya, Takaaki*; Ohara, Takashi*; Kurihara, Kazuo; Tomoyori, Katsuaki*; Tanaka, Ichiro*; et al.

Journal of Structural Biology, 177(2), p.283 - 290, 2012/02

 Times Cited Count:47 Percentile:83.59(Biochemistry & Molecular Biology)

Journal Articles

Neutron structure analysis using the IBARAKI biological crystal diffractometer (iBIX) at J-PARC

Tanaka, Ichiro*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; Niimura, Nobuo*; Ohara, Takashi*; Kurihara, Kazuo; Yamada, Taro*; Onishi, Yuki*; Tomoyori, Katsuaki*; Yokoyama, Takeshi*

Acta Crystallographica Section D, 66(11), p.1194 - 1197, 2010/11

 Times Cited Count:48 Percentile:94.66(Biochemical Research Methods)

The IBARAKI Biological Crystal Diffractometer (iBIX), a new diffractometer for protein crystallography at the next-generation neutron source at J-PARC (Japan Proton Accelerator Research Complex), has been constructed and has been operational since December 2008. Preliminary structure analyses of organic crystals showed that iBIX has high performance even at 120 kW operation and the first full data set is being collected from a protein crystal.

Journal Articles

Beginning of open use of a New Biological Neutron Diffractometer (iBIX) in J-PARC

Tanaka, Ichiro*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; Ohara, Takashi*; Kurihara, Kazuo; Niimura, Nobuo*

Yakugaku Zasshi, 130(5), p.665 - 670, 2010/05

 Times Cited Count:0 Percentile:0.02(Pharmacology & Pharmacy)

Ibaraki Prefectural Government together with Ibaraki University and Japan Atomic Energy Agency (JAEA) has almost finished constructing a time-of-flight (TOF) neutron diffractometer for biological macromolecules for industrial use at J-PARC, IBARAKI Biological Crystal Diffractometer (iBIX). Since 2009, Ibaraki University has been asked to operate this machine in order for users to do experiments by Ibaraki Prefecture. The diffractometer is designed to cover sample crystals which have their cell edges up to around 150 ${AA}$. It is expected to measure more than 100 samples per year if they have 2 mm$$^{3}$$ in crystal volume, and to measure even around 0.1 mm$$^{3}$$ in crystal volume of biological samples. The efficiency of iBIX is also expected about 100 times larger than those of the present high performance diffractometers at JRR-3 in JAEA when 1MW power realizes in J-PARC. Since December 2008, iBIX has been open to users and several proteins and organic compounds were tested under 20 kW proton power of J-PARC. It was found that one of their proteins was diffracted up to 1.4 ${AA}$ in d-spacing, which was nearly comparable resolution to that of BIX-3 in JRR-3 when used the same crystal as at iBIX for reasonable exposure time. In May 2009, 14 detector units were set up. By the end of fiscal year 2009, the basic part of data reduction software will be finished and an equipment blowing low temperature gas to the sample will be installed with the cooperation of JAEA.

Journal Articles

Single-crystal neutron diffraction analysis in chemistry

Hosoya, Takaaki*; Ohara, Takashi

Radioisotopes, 59(4), p.279 - 287, 2010/04

no abstracts in English

Journal Articles

Direct observation of crystalline-state reactions by single crystal neutron diffraction analyses

Ohara, Takashi; Hosoya, Takaaki; Ohashi, Yuji*

Nihon Kessho Gakkai-Shi, 50(1), p.98 - 102, 2008/02

The exchange of some hydrogen atoms with the other hydrogen atoms in the crystalline-state reactions, in which the reaction proceeds with retention of the single crystal form, can be directory observed by single crystal neutron diffraction technique if only the transferred hydrogen atoms were replaced with deuterium atoms before the photoirradiation. The method was successfully applied in the photoisomerization of three kinds of cobalt complex crystals and the mechanism in each reaction has been made clear.

Journal Articles

Hydrogen migration mechanism in crystalline-state photoisomerization by analyzed neutron diffraction

Ohashi, Yuji*; Hosoya, Takaaki; Ohara, Takashi

Crystallography Reviews, 12(2), p.83 - 123, 2006/04

The crystal structure analysis with neutron diffraction can clearly make a distinction between the deuterium and hydrogen atoms although they cannot be distinguished by X-rays. Applying the neutron diffraction for the crystalline-state reaction, the mechanisms of the hydrogen transfer in several reactions have been made clear.

Journal Articles

Neutron diffraction analysis of deuterium transfer in chiral $$beta$$-thiolactam formation in the crystalline state

Hosoya, Takaaki*; Uekusa, Hidehiro*; Ohashi, Yuji*; Ohara, Takashi; Tanaka, Ichiro*; Niimura, Nobuo*

Acta Crystallographica Section B; Structural Science, Crystal Engineering and Materials (Internet), 62(1), p.153 - 160, 2006/02

 Times Cited Count:5 Percentile:48.77(Chemistry, Multidisciplinary)

Since N,N-dibenzyl-1-cyclohexenecarbothioamide is photoisomerized to the optically active $$beta$$-thiolactam with the retention of the single-crystal form, the mechanism of chirality induction was identified by X-ray crystal structure analyses during the process of the reaction. In order to clarify the mechanism of hydrogen transfer in the reaction, the H atomsof the benzyl groups were replaced with deuterium atoms. The crystal structure after photoisomerization was analyzed by neutron diffraction. One of four deuterium atoms of the two benzyl groups is transferred to the C atom of the cyclohexene ring. The absolute configuration of the -C$$^{*}$$HD- group(chiral methylene) in the photoproduct r-thiolactam revealed that the deuterium atom occupies the equatorial position. This suggests that the deuterium atom is not transferred from the benzyl group of a neighbouring molecule, but from one of the benzyl groups within the molecule.

Journal Articles

Formation of a single-bunch beam in the booster synchrotron at SPring-8

Suzuki, Hiromitsu; Aoki, Tsuyoshi*; Ego, Hiroyasu*; Hara, Masahiro*; Hosoya, Naoyasu*; Kawashima, Yoshitaka*; Ohashi, Yuji*; Oshima, Takashi*; Tani, Norio; Yabashi, Makina*; et al.

Nuclear Instruments and Methods in Physics Research A, 444(3), p.515 - 533, 2000/04

 Times Cited Count:6 Percentile:44.21(Instruments & Instrumentation)

no abstracts in English

Oral presentation

Design of the neutron optics for Ibaraki biomolecular diffractometer

Ohara, Takashi; Kurihara, Kazuo; Kusaka, Katsuhiro; Hosoya, Takaaki; Tanaka, Ichiro*; Niimura, Nobuo*; Ozeki, Tomoji*; Aizawa, Kazuya; Morii, Yukio; Arai, Masatoshi; et al.

no journal, , 

no abstracts in English

Oral presentation

Current status of IBARAKI biological diffractometer in J-PARC; Optimization of design parameters

Kusaka, Katsuhiro; Ohara, Takashi; Tanaka, Ichiro*; Niimura, Nobuo*; Kurihara, Kazuo; Hosoya, Takaaki; Ozeki, Tomoji*; Aizawa, Kazuya; Morii, Yukio; Arai, Masatoshi; et al.

no journal, , 

Ibaraki prefecture has started to construct IBARAKI biological crystal diffractometer for industrial use at MLF, J-PARC. It is designed to achieve the efficiency which is more than 50 times larger than the present high performance diffractometer BIX-4. To realize this performance, the diffractometer will be installed on a coupled moderator has more intense peak but wider pulse shape than a decoupled one. It is expected that some neighbor Bragg spots will overlap partially each other along the time axis. The overlapping of Bragg spots should be considered for optimization of design parameters and it is necessary to de-convolute the overlapped spots. The three original simulation programs of TOF diffraction data with designed parameters of the diffractometer were developed to obtain information of spot-overlapping, completeness of Bragg spots and spot profiles along the time axis. The consideration of important designed parameters will be reported based on the simulation results.

Oral presentation

Optimization of designparameters for Ibaraki Biological diffractometer in J-PARC by simulations of TOF diffraction data

Kusaka, Katsuhiro; Ohara, Takashi; Tanaka, Ichiro*; Niimura, Nobuo*; Kurihara, Kazuo; Hosoya, Takaaki; Ozeki, Tomoji*; Aizawa, Kazuya; Morii, Yukio; Arai, Masatoshi; et al.

no journal, , 

no abstracts in English

Oral presentation

Current status of IBARAKI biological diffractometer in J-PARC; Design of the neutron optics

Ohara, Takashi; Kurihara, Kazuo; Kusaka, Katsuhiro; Hosoya, Takaaki; Tanaka, Ichiro*; Niimura, Nobuo*; Ozeki, Tomoji*; Aizawa, Kazuya; Morii, Yukio; Arai, Masatoshi; et al.

no journal, , 

Ibaraki Prefectural Government in Japan has started to construct a TOF single crystal neutron diffractometer for biological macromolecules for industrial use at J-PARC. For this diffractometer, design of an efficient neutron transportation system is important because this diffractometer has 40m source-sample distance. Recently, we designed a supermirror neutron guide which can transport 0.7-3.8 Angstrom neutron efficiently. The total length of the mirror section is 25m. At the first 17m, the mirror has curvature (R=4300m) for horizontal direction in order to remove high-energy neutron and $$gamma$$ ray. Simultaneously, all of the mirror section has tapered angle for vertical direction in order to reduce the frequency of neutron reflection at the surface of the supermirror. The neutron flux and profile at the sample position was calculated by Monte Carlo simulation softwares, McStas and IDEAS and compared with a curved, non-tapered guide we had designed previously. In result, the new supermirror system has 2 times gain for 0.7 Angstrom neutron and 1.5 times for 1.5 Angstrom neutron, and the beam profile at the sample position has a rectangular shape.

46 (Records 1-20 displayed on this page)