Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Sakamoto, Fuminori; Onuki, Toshihiko; Kozai, Naofumi; Igarashi, Shosuke*; Yamasaki, Shinya; Yoshida, Zenko; Tanaka, Shunichi*
Nihon Genshiryoku Gakkai Wabun Rombunshi, 11(1), p.1 - 7, 2012/01
The autoradiography analyses of plants and soils collected in Fukushima showed radioactive Cs was distributed on the branch and leaves of trees that were present at the accident and that only small fraction may be transported to new branch and leaves grown after the accident. Radioactive Cs was present on the grass and rice stubble on the soils, but not in the soils beneath the grass and rice stubble, indicating that the radioactive Cs was deposited on the grass and the rice plant. In addition the ratio of the radioactive Cs penetrated into soil layer by weathering was very small for two months after the accident. These results indicate that trees and plant would be the reservoir of the fallout Cs and function for retardation of the fallout Cs migration with rain water.
ray irradiation at base pair levelSakamoto, Fuminori; Igarashi, Shosuke*; Onuki, Toshihiko
no journal, ,
It is known that DNA is damaged by the radiation, and many researchers studied the type of DNA damage, the DNA repair mechanism, and so on. However, identification of scission site and specificity of scission site have not been studied in detail up to now. We, therefore, cleaved DNA by irradiation of 
Co ray, and specified the scission sites at one base pair level by using the technique that we developed. We considered the relationship between the scission sites and the type of base as well as DNA secondary structure.
Onuki, Toshihiko; Sakamoto, Fuminori; Kozai, Naofumi; Igarashi, Shosuke*; Yamasaki, Shinya; Yoshida, Zenko; Tanaka, Shunichi*
no journal, ,
Radioactive Cs was present on the grass and rice stubble on the soils, but not in the soils beneath the grass and rice stubble, indicating that the radioactive Cs was deposited on the grass and the rice plant. In addition the ratio of the radioactive Cs penetrated into soil layer by weathering was very small for two months after the accident. These results indicate that trees and plant would be the reservoir of the fallout Cs and function for retardation of the fallout Cs migration with rain water.
Igarashi, Shosuke*; Onuki, Toshihiko; Sakamoto, Fuminori; Tachibana, Akira*
no journal, ,
Effects of radiation on the cisplatin associated DNA have been studied in base level by using Sanger method. The results indicated that presence of cisplatin enhanced strand break by X-ray irradiation, and positions of the strand break bases are related to the association of cisplatin with bases.
Igarashi, Shosuke; Onuki, Toshihiko; Sakamoto, Fuminori
no journal, ,
We have found specific base positions in the DNA cleavage by the irradiation of X-rays after association of a cisplatin with DNA. In cancer therapy it is well known that application of X-rays radiation with cisplatin dosage enhances the anti-cancer effect. However, the mechanism of enhancement in the anti-cancer has not been elucidated in the level of DNA base. We have developed a new approach for the determination of the DNA cleavage position using the Sanger method. Here we have specified the positions of base cleaved by X-ray radiation with cisplatin association with DNA. The cleavage patterns of the DNA irradiated by X-ray show that the DNA was cleaved by 100 Gy irradiation, and the four kinds of bases were cleaved. Ciplatin associated DNA without the irradiation was not cleaved. On the other hand, the ciplatin associated DNA specifically enhanced the cleavage at the bases of "guanine" and "adenine". When the irradiation increased to 150 Gy, the specific cleavage at the "guanine" and "adenine" were still detected. These results indicate that supra-additive effect by cisplatin association and X-ray irradiation on the DNA damage was caused by the specific cleavage at the base positions of "guanine" and "adenine".
