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Journal Articles

Autoradiography system with phosphor powder (ZnS:Ag) for imaging radioisotope dynamics in a living plant

Kurita, Keisuke; Sakai, Takuro; Suzui, Nobuo*; Yin, Y.-G.*; Sugita, Ryohei*; Kobayashi, Natsuko*; Tanoi, Keitaro*; Kawachi, Naoki*

Japanese Journal of Applied Physics, 60(11), p.116501_1 - 116501_4, 2021/11

 Times Cited Count:1 Percentile:8.23(Physics, Applied)

Radioisotope tracer imaging is useful for studying plant physiological phenomena. In this study, we developed an autoradiography system with phosphor powder (ZnS:Ag), "Live-autoradiography", for imaging radioisotope dynamics in a living plant. This system visualizes the element migration and accumulation in intact plants continuously under a light environment. An imaging test was performed on point sources of $$^{137}$$Cs, with a radioactivity of 10-100 kBq of being observed; this indicates satisfactory system linearity between the image intensity and the radioactivity of $$^{137}$$Cs. Moreover, dynamics imaging of $$^{137}$$Cs was performed on an intact soybean plant for four days. The serial images indicated $$^{137}$$Cs accumulation in the node, vein, and growing point of the plant. The developed system can be used for studying plant physiological phenomena and can be employed for quantitative measurement of radionuclides.

Journal Articles

Celebration of 30th anniversary of the experimental fast reactor Joyo

Nakai, Satoru; Aoyama, Takafumi; Ito, Chikara; Yamamoto, Masaya; Iijima, Minoru; Nagaoki, Yoshihiro; Kobayashi, Atsuko; Onoda, Yuichi; Ohgama, Kazuya; Uwaba, Tomoyuki; et al.

Kosoku Jikkenro "Joyo" Rinkai 30-Shunen Kinen Hokokukai Oyobi Gijutsu Koenkai, 154 Pages, 2008/06

no abstracts in English

Oral presentation

Characterization of a novel Cd sensitive mutant in Arabidopsis

Nakamura, Atsuko*; Ichikawa, Kazuki*; Kojima, Kazuaki*; Ono, Yutaka; Kobayashi, Yasuhiko; Tanaka, Shigeo*; Sakata, Yoichi*

no journal, , 

Phytochelatin (PC) has been shown to play an important role in heavy metal detoxification. However, Arabidopsis transgenic plants overexpressing the PC synthase gene failed to show an increase in Cd tolerance. To explore a novel heavy metal detoxification mechanism, we screened for an Arabidopsis Cd-sensitive mutant (${it cds1}$) by carbon ion beam mutagenesis, which causes large base pair deletions at random loci in the genome. Genetic analyses and GSH synthesis inhibitor studies revealed that the ${it cds1}$ phenotype was different from those of PC synthesis mutants. Moreover, Cd content in shoots of ${it cds1}$ cultured on Cd-containing media was similar to wild type, suggesting that Cd-sensitive phenotype of ${it cds1}$ was not attributed to the Cd transport system from roots to shoots. In the rough mapping, ${it cds1}$ was localized to approximately 300kbp region of chromosome 4. One of the genes was found to have a 2.5 kbp deletion in the promoter region.

Oral presentation

Evaluation of labile Cs-137 in Fukushima rivers by diffusive gradients in thin films (DGT, 2; Examination in river basins

Tanaka, Takuro*; Saito, Takumi*; Toda, Kanako*; Fujiwara, Kenso; Terashima, Motoki; Nakanishi, Takahiro; Kobayashi, Natsuko*; Tanoi, Keitaro*

no journal, , 

Cs-137 dispersed by the Fukushima Daiichi Nuclear Power Plant accident deposited in Fukushima area, that are not decontaminated in mountainous areas, may migrate through river water to animals and plants. Most of Cs-137 in river are fixed in clay minerals, but there are some Cs that can be easily desorbed, named as labile components. It has been suggested that labile components affect the bioavailability of Cs-137. In this study, the labile Cs-137 was sampled in situ from upstream to downstream in several rivers of Fukushima using a passive sampler called diffusive gradients in thin films (DGT). Sampling was conducted at a different season in addition to the previous studies.

Oral presentation

Evaluation of labile $$^{137}$$Cs in Fukushima rivers by diffusive gradients in thin films (DGT), 3; Relations between geochemical parameters

Tanaka, Takuro*; Saito, Takumi*; Toda, Kanako*; Fujiwara, Kenso; Terashima, Motoki; Nakanishi, Takahiro; Kobayashi, Natsuko*; Tanoi, Keitaro*; Kato, Hiroaki*

no journal, , 

$$^{137}$$Cs dispersed by the Fukushima Daiichi Nuclear Power Plant accident deposited in Fukushima area, that are not decontaminated in mountainous areas, may migrate through river water to animals and plants. Most of $$^{137}$$Cs in river are fixed in clay minerals, but there are some Cs that can be easily desorbed, named as labile components. It has been suggested that labile components affect the bioavailability of $$^{137}$$Cs. In this study, the labile $$^{137}$$Cs was sampled in situ from upstream to downstream in several rivers of Fukushima using a passive sampler called diffusive gradients in thin films (DGT). The desorption behavior of the labile component and factors affecting it will be discussed by examining the relationship with particulate $$^{137}$$Cs and geochemical parameters in river water.

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