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Shibata, Masahiro; Sawada, Atsushi; Tachi, Yukio; Makino, Hitoshi; Wakasugi, Keiichiro; Mitsui, Seiichiro; Kitamura, Akira; Yoshikawa, Hideki; Oda, Chie; Ishidera, Takamitsu; et al.
JAEA-Research 2014-030, 457 Pages, 2015/03
JAEA-Research-2014-030.pdf:199.23MB
JAEA and NUMO have conducted a collaborative research work which is designed to enhance the methodology of repository design and post-closure performance assessment in preliminary investigation stage. With regard to (1) study on rock suitability in terms of hydrology, based on some examples of developing method of hydro-geological structure model, acquired knowledge are arranged using the tree diagram, and model uncertainty and its influence on the evaluation items were discussed. With regard to (2) study on scenario development, the developed approach for "defining conditions" has been reevaluated and improved from practical viewpoints. In addition, the uncertainty evaluation for the effect of use of cementitious material, as well as glass dissolution model, was conducted with analytical evaluation. With regard to (3) study on setting radionuclide migration parameters, based on survey of precedent procedures, multiple-approach for distribution coefficient of rocks was established, and the adequacy of the approach was confirmed though its application to sedimentary rock and granitic rock. Besides, an approach for solubility setting was developed including the procedure of selection of solubility limiting solid phase. The adequacy of the approach was confirmed though its application to key radionuclides.
Shibata, Masahiro; Sawada, Atsushi; Tachi, Yukio; Hayano, Akira; Makino, Hitoshi; Wakasugi, Keiichiro; Mitsui, Seiichiro; Oda, Chie; Kitamura, Akira; Osawa, Hideaki; et al.
JAEA-Research 2013-037, 455 Pages, 2013/12
JAEA-Research-2013-037.pdf:42.0MB
Following FY2011, JAEA and NUMO have conducted a collaborative research work which is designed to enhance the methodology of repository design and performance assessment in preliminary investigation stage. With regard to (1) study on rock suitability in terms of hydrology, the tree diagram of methodology of groundwater travel time has been extended for crystalline rock, in addition, tree diagram for sedimentary rock newly has been organized. With regard to (2) study on scenario development, the existing approach has been improved in terms of a practical task, and applied and tested for near field focusing on the buffer. In addition, the uncertainty of some important processes and its impact on safety functions are discussed though analysis. With regard to (3) study on setting radionuclide migration parameters, the approaches for parameter setting have been developed for sorption for rocks and solubility, and applied and tested through parameter setting exercises for key radionuclides.
Shibata, Masahiro; Sawada, Atsushi; Tachi, Yukio; Makino, Hitoshi; Hayano, Akira; Mitsui, Seiichiro; Taniguchi, Naoki; Oda, Chie; Kitamura, Akira; Osawa, Hideaki; et al.
JAEA-Research 2012-032, 298 Pages, 2012/09
JAEA-Research-2012-032.pdf:33.68MB
JAEA and NUMO have conducted a collaborative research work which is designed to enhance the methodology of repository design and performance assessment in preliminary investigation phase. The topics and the conducted research are follows; (1) Study on selection of host rock: in terms of hydraulic properties, items for assessing rock property, and assessment methodology of groundwater travel time has been organized with interaction from site investigation. (2) Study on development of scenario: the existing approach has been embodied, in addition, the phenomenological understanding regarding dissolution of and nuclide release from vitrified waste, corrosion of the overpack, long-term performance of the buffer are summarized. (3) Study on setting nuclide migration parameters: the approach for parameter setting has been improved for sorption and diffusion coefficient of buffer/rock, and applied and tested for parameter setting of key radionuclides. (4) Study on ensuring quality of knowledge: framework for ensuring quality of knowledge has been studied and examined aimed at the likely disposal facility condition.
-sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for -form prion proteinKubota, Toshiya*; Hamazoe, Yuta*; Hashiguchi, Shuhei*; Ishibashi, Daisuke*; Akasaka, Kazuyuki*; Nishida, Noriyuki*; Katamine, Shigeru*; Sakaguchi, Suehiro*; Kuroki, Ryota; Nakashima, Toshihiro*; et al.
Journal of Biological Chemistry, 287(17), p.14023 - 14039, 2012/04
Times Cited Count：5 Percentile：11.64(Biochemistry & Molecular Biology)We prepared -sheet-rich recombinant full-length prion protein (-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this -form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to -form but not 
-form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates. The SAF32 antibody recognizing the N-terminal octarepeat region of full-length PrP stained distinct granules in these cells as determined by confocal laser microscopy observation. When the accumulation of proteinase K-resistant PrP was examined in prion-infected ScN2a cells cultured in the presence of PRB7 IgG or SAF32, it was strongly inhibited by SAF32 but not at all by PRB7 IgG. Thus, we demonstrated direct evidence of the generation and accumulation of -sheet-rich PrP in ScN2a cells de novo. These results suggest first that PRB7-bound PrP is not responsible for the accumulation of -form PrP aggregates, which are rather an end product resulting in the triggering of apoptotic cell death, and second that SAF32-bound PrP lacking the PRB7-recognizing -form may represent so-called PrPSc with prion propagation activity. PRB7 is the first human antibody specific to -form PrP and has become a powerful tool for the characterization of the biochemical nature of prion and its pathology.
Sugihara, Yutaka*; Kubota, Shigeru*; Yamamoto, Takuya*; Kamemura, Katsumi*; *; *
JNC TJ1400 2001-005, 383 Pages, 2003/03
JNC-TJ1400-2001-005.pdf:18.73MB
no abstracts in English
Nobuto, Jun*; Noda, Masaru*; Toida, Masaru*; Kubota, Shigeru*
JNC TJ7400 2002-010, 379 Pages, 2003/02
JNC-TJ7400-2002-010.pdf:27.97MB
The Mizunami Underground Research Laboratory, which Japan Nuclear Cycle Development Institute (hereafter, JNC) plans to construct for expanding further the geoscientific researches being conducted at Tono Geoscience Center and the vicinity, consists of the surface facilities and the underground research facilities extending up to the depth of 1000m. This study is conducted to investigate engineering technologies related to the construction of the Underground Research Laboratory, since the construction of the laboratory started in fiscal 2002. Concretely, following four items were studied. (1)Study on the technologies for feeding back measurement results to the design and the construction scheme (2)Study on the technologies for securing the qualities of research and construction (3)Study on construction technologies to treat the outbreak of unexpected water inflow (4)Study on the influence of earthquakes on underground research facilities. The former three items are related to the construction technologies under the deep subterranean environment.
Kubota, Shigeru*; *; Noda, Masaru*; Furuichi, Mitsuaki*; *
JNC TJ1400 2002-003, 1053 Pages, 2003/02
JNC-TJ1400-2002-003.pdf:55.13MB
none
Toida, Masaru*; Noda, Masaru*; *; Kubota, Shigeru*
JNC TJ7400 2002-001, 519 Pages, 2002/03
JNC-TJ7400-2002-001.pdf:19.21MB
no abstracts in English
Ijiri, Yuji*; Hattori, Hiromichi*; Suzuki, Shunichi*; Oishi, Masaya*; Kubota, Shigeru*; Adachi, Tetsuya*; Yamamoto, Takuya*
JNC TJ8400 2001-010, 143 Pages, 2001/03
JNC-TJ8400-2001-010.pdf:17.19MB
The evaluation of uncertainty of geological environment is a key issue in assessing a performance of a deep geological disposal system for high-level nuclear waste. The geological environment uncertainty is arose from data obtained from surveys and model used for a performance assessment.The objectives of this study is to develop a rational planning methodology of site characterization using the Horonobe site as a model case, and to establish a modeling methodology using the Tono site as a model case. At the Horonobe site, first, a conceptual model is constructed based on the information at every stage of site characterization procedure. A sensitivity analysis is performed using various conceptual models and data. If conceptual models uncertainty have a great impact on the result of performance assessment analysis, surveys that detect the most appropriate conceptual model are conducted in the next stage of site characterization. If data uncertainty have a great impact on the result of performance assessment analysis, surveys that decrease the data uncertainty are conducted. Thus, a rational site characterization methodology in which the most sensitive items are preferentially detected in the next stage of site characterization is developed. This concept of a rational site characterization methodology is described in this report. In addition, preliminary conceptual model is developed based on geological information around Horonobe site, and data uncertainty for the sensitivity analysis which will be conducted in next year is investigated. At the Tono site, literature on model uncertainty is surveyed, and data obtained at the Tono site is reviewed from modeling point of view.
Ijiri, Yuji*; Hattori, Hiromichi*; Suzuki, Shunichi*; Oishi, Masaya*; Kubota, Shigeru*; Adachi, Tetsuya*; Yamamoto, Takuya*; Igarashi, Takafumi*; Sugihara, Yutaka*
JNC TJ8400 2001-009, 41 Pages, 2001/03
JNC-TJ8400-2001-009.pdf:6.69MB
no abstracts in English
Kubota, Shigeru*; Noda, Masaru*; Toida, Masaru*; *
JNC TJ7400 2001-005, 290 Pages, 2001/03
JNC-TJ7400-2001-005.pdf:8.83MB
no abstracts in English
; Taniguchi, Wataru; Koo, Shigeru*; Hasegawa, Hiroshi; Sugino, Hiroyuki; Kubota, Shigeru*; Dewa, Katsuyuki*
JNC TN8400 99-037, 281 Pages, 1999/11
It is planned to construct the tunnels and emplace waste packages at several hundred meters to 1,000 meters under the ground for the repository of high-level radioactive waste based on a policy to assure the safe life environment. It is required to be mechanically stable for the tunnels to assure the work safety throughout the construction, operation and closure phase. In this report, the mechanical stability of tunnels, that is a factor of design requisites, was evaluated by the analyses to present an outline of the technical reliability of geological disposal. To put it concretely, the tunnel sections were determined to have the required areas and shapes, and the analyses on the mechanical stability at tunnel excavations and earthquake, at tunnel intersections were conducted by the theoretical analysis and finite element method. The results obtained by these investigations are shown below: (1)It will be able to construct the tunnels with present techniques. The mechanical stability of tunnels will be assured if proper supports are given, and adequate tunnel spacing and disposal-pit pitches are set. (2)The mechanical stability will be assured at the tunnels intersections if proper reinforcement measures are taken. The reinforcement will be required for the intersection areas over the distance of 1D (D: diameter of tunnels) on the obtuse angle side, and 4D on the acute angle side, when intersection angle is set at 30 degrees. (3)The investigations were conducted on the assumption that the experienced big earthquake occurred. The results show that the effect of earthquake on the mechanical stability of tunnels is small, and tunnels are stable at the earthquake when the mechanical stability at tunnel excavations is assured.
Anezaki, Susumu*; Sugihara, Yutaka*; Igarashi, Takafumi*; Kubota, Shigeru*; Adachi, Tetsuya*
JNC TJ1410 98-001, 74 Pages, 1998/10
As a technical problem to be solved in the underground research of sedimentary rock formation, the specification for access drift from the surface to the experimental area in the underground was studied. Considering ground condition, safety for construction and experiment execution, maintenance of experimental environment, construction period and cost, the feasibility of access drift was carried out.
Kubota, Shigeru*; *; Okutsu, Kazuo*; Horita, M.*; Amemiya, Kiyoshi*
PNC TJ1449 98-001, 1944 Pages, 1998/02
None
Kubota, Masumitsu; Dojiri, Shigeru; Yamaguchi, Isoo; Morita, Yasuji; ; *; *
Proc. of the 1989 Joint Int. Waste Management Conf., Vol. 2, p.537 - 542, 1989/00
no abstracts in English
*; Dojiri, Shigeru; Kubota, Masumitsu
JAERI-M 88-188, 116 Pages, 1988/10
no abstracts in English
Hayano, Akira; Sawada, Atsushi; Goto, Junichi*; Ishii, Eiichi*; Moriya, Toshifumi*; Inagaki, Manabu*; Kubota, Shigeru*; Ebashi, Takeshi*
no journal, ,
Detailed Investigation Areas have to be selected with focusing on suitability of the host rock in the siting process for high-level radioactive waste disposal in Japan. The suitability of the host rock is evaluated in terms of the preferable disposal condition such as thermal, hydraulic, mechanical and geochemical condition, and the feasibility of the geological disposal project relevant to volumetric capacity of host rock and economics, based on the site model. However, due to a limited number of surveys at relatively early stage of the investigation, the lack of understanding of geological environment at the site causes an uncertainty of the site model. It is essential to clarify an association the important factors relevant to safety assessment (SA) and a repository design (Design) with the information obtained by the site investigation through the site model. In this study, the methodology not only for defining evaluation factors relevant to SA in term of hydrogeology, but also for visualizing the methods to evaluate the factors through the site model is organized into a tree diagram. A method to evaluate groundwater travel time which is one of evaluation factors in granitic rock is organized into a first version of the tree diagram.
