JOPSS - Search Results

Journal Articles

The Catalytic mechanism of decarboxylative hydroxylation of salicylate hydroxylase revealed by crystal structure analysis at 2.5 resolution

Uemura, Takuya*; Kita, Akiko*; Watanabe, Yoshihiko*; Adachi, Motoyasu; Kuroki, Ryota; Morimoto, Yukio*

Biochemical and Biophysical Research Communications, 469(2), p.158 - 163, 2016/01

https://doi.org/10.1016/j.bbrc.2015.11.087

Times Cited Count：16 Percentile：50.44(Biochemistry & Molecular Biology)

Journal Articles

An Approach to DNA crystallization using the thermal reversible process of DNA duplexes

Chatake, Toshiyuki*; Sazaki, Gen*; Kikko, Tatsuhiko*; Fujiwara, Satoru; Ishikawa, Takuya*; Matsumoto, Osamu*; Morimoto, Yukio*

Crystal Growth & Design, 10(3), p.1090 - 1095, 2010/03

https://doi.org/10.1021/cg9007075

Times Cited Count：4 Percentile：48.31(Chemistry, Multidisciplinary)

We propose a technique for DNA crystallization using the thermal reversible process of DNA: a conversion between a double-stranded DNA (dsDNA) and two single-stranded DNAs (ssDNAs) with temperatures. We investigated the solubility of the crystals of a DNA hexamer d(CGCGCG) and their melting temperature, at which the thermal conversion occurs. The results obtained suggest that the conversion from a dsDNA to ssDNAs results in an increase in solubility. It was shown that using this temperature-controlled technique, high-grade single crystals of the DNA hexamer could be obtained from a small amount of DNA samples. This easy-to-apply technique would be superior to the conventional vapor diffusion technique in that it allows the solubility of DNA crystals to be controlled with no need for expensive setups.

Journal Articles

Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography

Chatake, Toshiyuki*; Shibayama, Naoya*; Park, S. Y.*; Kurihara, Kazuo; Tamada, Taro; Tanaka, Ichiro*; Niimura, Nobuo*; Kuroki, Ryota; Morimoto, Yukio*

Journal of the American Chemical Society, 129(48), p.14840 - 14841, 2007/12

https://doi.org/10.1021/ja0749441

Times Cited Count：25 Percentile：60.26(Chemistry, Multidisciplinary)

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