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Nakaniwa, Tetsuko*; Fukata, Harumi*; Inoue, Tatsuya*; Goda, Masaki*; Nakai, Ryoko*; Kirii, Yasuyuki*; Adachi, Motoyasu; Tamada, Taro; Segawa, Shinichi*; Kuroki, Ryota; et al.
Biochemistry, 51(42), p.8410 - 8421, 2012/10
Times Cited Count：18 Percentile：42.99(Biochemistry & Molecular Biology)Protein kinase is a vital drug target for the treatment of a wide range of diseases. To investigate the effect of cysteine mutation on the function, stability and structure of kinase, free cysteines of c-Jun N-terminal kinase 1 (JNK1) were systematically removed by mutation. Two cysteine-destructed mutants in which three (M3) and seven (M7) cysteine residues are removed, yielded about 5 and 2 times than wild type JNK-1 (M0). SDS PAGE analysis showed that the aggregation was less in the case of M3 and M7. Thermal unfolding experiment of M0, M3 and M7 using by differential scanning calorimetry proceeded at least three state unfolding. Crystal structure of the M3 mutant was determined to 2.6 
resolution, which was identical to that of the wild-type. Consequently, due to the highest yield, its improved stability against aggregation and its structural similarity to the wild type, the M3 mutant is suitable for the use of further characterization of its function and structure.
Sakanaka, Shogo*; Akemoto, Mitsuo*; Aoto, Tomohiro*; Arakawa, Dai*; Asaoka, Seiji*; Enomoto, Atsushi*; Fukuda, Shigeki*; Furukawa, Kazuro*; Furuya, Takaaki*; Haga, Kaiichi*; et al.
Proceedings of 1st International Particle Accelerator Conference (IPAC '10) (Internet), p.2338 - 2340, 2010/05
Future synchrotron light source using a 5-GeV energy recovery linac (ERL) is under proposal by our Japanese collaboration team, and we are conducting R&D efforts for that. We are developing high-brightness DC photocathode guns, two types of cryomodules for both injector and main superconducting (SC) linacs, and 1.3 GHz high CW-power RF sources. We are also constructing the Compact ERL (cERL) for demonstrating the recirculation of low-emittance, high-current beams using above-mentioned critical technologies.
Kusakabe, Toshio*; Miyamoto, Yoshiharu*; Ishida, Rikiya*; Ito, Kojiro*; Kuroyanagi, Nobuhiro*; Nakai, Yota*; Shirai, Toshizo
Nuclear Instruments and Methods in Physics Research B, 205, p.600 - 604, 2003/05
Times Cited Count：6 Percentile：43.07(Instruments & Instrumentation)no abstracts in English
Shirai, Toshizo; *; Nakai, Yota; Sugar, J.*; *; *
Journal of Physical and Chemical Reference Data, 20(1), p.1 - 81, 1991/00
no abstracts in English
Shirai, Toshizo; *; *; Sugar, J.*; Wiese, W. L.*; Nakai, Yota
Journal of Physical and Chemical Reference Data, 19(1), p.127 - 275, 1990/00
Times Cited Count：94 Percentile：87.33(Chemistry, Multidisciplinary)no abstracts in English
with H
,O,CH
,CO and COSataka, Masao; *; Nakai, Yota
Journal of Physics B; Atomic, Molecular and Optical Physics, 23, p.1225 - 1234, 1990/00
Times Cited Count：29 Percentile：79(Optics)no abstracts in English
Kawatsura, Kiyoshi*; Sataka, Masao; *; Komaki, Kenichiro*; Kanai, Yasuyuki*; Naramoto, Hiroshi; *; Kambara, Tadashi*; *; Nakai, Yota; et al.
Nuclear Instruments and Methods in Physics Research B, 48, p.103 - 106, 1990/00
Times Cited Count：17 Percentile：82.1(Instruments & Instrumentation)no abstracts in English
C and Ne ions with Ne; Sataka, Masao; Kawatsura, Kiyoshi; Shirai, Toshizo; Nakai, Yota
Journal of Physics B; Atomic, Molecular and Optical Physics, 22, p.263 - 270, 1989/00
Times Cited Count：7 Percentile：44.97(Optics)no abstracts in English
andHeNakai, Yota; Shirai, Toshizo; Tabata, Tatsuo*; Ito, Rinsuke*
Phys. Scr., T28, p.77 - 80, 1989/00
Times Cited Count：18 Percentile：73.81(Physics, Multidisciplinary)no abstracts in English
Sataka, Masao; Ozawa, Kunio*; Kawatsura, Kiyoshi; *; Komaki, Kenichiro*; Naramoto, Hiroshi; *; Nakai, Yota; *
Journal of the Physical Society of Japan, 57(10), p.3352 - 3356, 1988/10
no abstracts in English
Tabata, Tatsuo*; Ito, Rinsuke*; Nakai, Yota; Shirai, Toshizo; Sataka, Masao; *
Nuclear Instruments and Methods in Physics Research B, 31, p.375 - 381, 1988/00
no abstracts in English
Naramoto, Hiroshi; Kawatsura, Kiyoshi; Sataka, Masao; ; Nakai, Yota; Ozawa, Kunio*; *; Fujino, Yutaka*; *
Nuclear Instruments and Methods in Physics Research B, 33, p.595 - 598, 1988/00
no abstracts in English
Shirai, Toshizo; *; Sugar, J.*; Wiese, W. L.*; Nakai, Yota; Ozawa, Kunio*
Atomic Data and Nuclear Data Tables, 37(2), p.235 - 332, 1987/00
Times Cited Count：27 Percentile：38.98(Physics, Atomic, Molecular & Chemical)no abstracts in English
Nakai, Yota; Shirai, Toshizo; Tabata, Tatsuo*; Ito, Rinsuke*
Atomic Data and Nuclear Data Tables, 37, p.69 - 101, 1987/00
Times Cited Count：92 Percentile：96.09(Physics, Atomic, Molecular & Chemical)no abstracts in English
Sn(
,
) reactionEndo, Shunsuke; Abe, Ryota*; Ishizaki, Kohei*; Oku, Takayuki; Okudaira, Takuya*; Kameda, Kento*; Kitaguchi, Masaaki*; Kimura, Atsushi; Sakai, Kenji; Shima, Tatsushi*; et al.
no journal, ,
no abstracts in English
-rays from the neutron capture reaction of Pd isotopes in the eV energy rangeYoshikawa, Hiromoto*; Abe, Ryota*; Ishizaki, Kohei*; Ito, Yuki*; Endo, Shunsuke; Oku, Takayuki; Okudaira, Takuya*; Kameda, Kento*; Kitaguchi, Masaaki*; Kimura, Atsushi; et al.
no journal, ,
no abstracts in English
Fujiie, Takuhiro*; Abe, Ryota*; Ishizaki, Kohei*; Ito, Yuki*; Endo, Shunsuke; Oku, Takayuki; Okudaira, Takuya*; Kameda, Kento*; Kitaguchi, Masaaki*; Kimura, Atsushi; et al.
no journal, ,
no abstracts in English
In(n,) reaction toward a search for time-reversal violation using an indium targetTakada, Shusuke*; Abe, Ryota*; Ishizaki, Kohei*; Ito, Yuki*; Endo, Shunsuke; Oku, Takayuki; Okudaira, Takuya*; Kameda, Kento*; Kitaguchi, Masaaki*; Kimura, Atsushi; et al.
no journal, ,
no abstracts in English
-ray measurement from the neutron capture reaction of Pd isotopes in the eV energy rangeYoshikawa, Hiromoto*; Abe, Ryota*; Ishizaki, Kohei*; Ito, Yuki*; Endo, Shunsuke; Oku, Takayuki; Okudaira, Takuya*; Kameda, Kento*; Kitaguchi, Masaaki*; Kimura, Atsushi; et al.
no journal, ,
no abstracts in English
