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Matsuda, Shohei; Nakashima, Nobuaki*; Yokoyama, Keiichi; Taniguchi, Seiji*; Chosrowjan, H.*; Somekawa, Toshihiro*; Yatsuhashi, Tomoyuki*
Chemical Physics Letters, 802, p.139759_1 - 139759_6, 2022/09
Times Cited Count：0 Percentile：0.01(Chemistry, Physical)no abstracts in English
-sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for -form prion proteinKubota, Toshiya*; Hamazoe, Yuta*; Hashiguchi, Shuhei*; Ishibashi, Daisuke*; Akasaka, Kazuyuki*; Nishida, Noriyuki*; Katamine, Shigeru*; Sakaguchi, Suehiro*; Kuroki, Ryota; Nakashima, Toshihiro*; et al.
Journal of Biological Chemistry, 287(17), p.14023 - 14039, 2012/04
Times Cited Count：5 Percentile：11.64(Biochemistry & Molecular Biology)We prepared -sheet-rich recombinant full-length prion protein (-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this -form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to -form but not 
-form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates. The SAF32 antibody recognizing the N-terminal octarepeat region of full-length PrP stained distinct granules in these cells as determined by confocal laser microscopy observation. When the accumulation of proteinase K-resistant PrP was examined in prion-infected ScN2a cells cultured in the presence of PRB7 IgG or SAF32, it was strongly inhibited by SAF32 but not at all by PRB7 IgG. Thus, we demonstrated direct evidence of the generation and accumulation of -sheet-rich PrP in ScN2a cells de novo. These results suggest first that PRB7-bound PrP is not responsible for the accumulation of -form PrP aggregates, which are rather an end product resulting in the triggering of apoptotic cell death, and second that SAF32-bound PrP lacking the PRB7-recognizing -form may represent so-called PrPSc with prion propagation activity. PRB7 is the first human antibody specific to -form PrP and has become a powerful tool for the characterization of the biochemical nature of prion and its pathology.
Takenaga, Hidenobu; Ogawa, Yuichi*; Takizuka, Tomonori; Yagi, Masatoshi*; Yamada, Hiroshi*; Sakamoto, Yoshiteru; Toi, Kazuo*; Fukuda, Takeshi*; Fukuyama, Atsushi*; Fujita, Takaaki; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 84(7), p.465 - 467, 2008/07
no abstracts in English
Sasao, Mamiko*; Kusama, Yoshinori; Kawano, Yasunori; Kawahata, Kazuo*; Mase, Atsushi*; Sugie, Tatsuo; Fujita, Takaaki; Fukuda, Takeshi*; Fukuyama, Atsushi*; Sakamoto, Yoshiteru; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 83(9), p.779 - 782, 2007/09
This is a report of highlights from 2007 spring meetings of seven Topical Groups (TG) of International Tokamak Physics Activity (ITPA). In each meeting, high priority issues in physics of International Thermonuclear Experimental Reactor (ITER) and other burning plasma experiments have been discussed and investigated. Twenty-seven scientists from Japan have participated in those meetings. Dates and places of the meetings are shown below. (1) Diagnostics TG: 26-30 March, Princeton (USA), (2) Transport Physics TG: 7-10 May, Lausanne (Switzerland), (3) Confinement Database and Modeling TG: 7-10 May, Lausanne (Switzerland), (4) Edge Pedestal Physics TG: 7-10 May, Garching (Germany) (5) Steady State Operation TG: 9-11 May, Daejeon (South Korea), (6)MHD TG: 21-24 May, San Diego (USA), (7) Scrape-off-layer and Divertor Physics TG: 7-10 May, Garching (Germany).
Kawahata, Kazuo*; Kawano, Yasunori; Kusama, Yoshinori; Mase, Atsushi*; Sasao, Mamiko*; Sugie, Tatsuo; Fujita, Takaaki; Fukuda, Takeshi*; Fukuyama, Atsushi*; Sakamoto, Yoshiteru; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 83(2), p.195 - 198, 2007/02
no abstracts in English
Asakura, Nobuyuki; Kato, Takako*; Nakano, Tomohide; Takamura, Shuichi*; Tanabe, Tetsuo*; Iio, Shunji*; Nakajima, Noriyoshi*; Ono, Yasushi*; Ozeki, Takahisa; Takechi, Manabu; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 82(7), p.448 - 450, 2006/07
no abstracts in English
Sakamoto, Yoshiteru; Toi, Kazuo*; Fukuda, Takeshi*; Fukuyama, Atsushi*; Fujita, Takaaki; Ogawa, Yuichi*; Takizuka, Tomonori; Takenaga, Hidenobu; Yagi, Masatoshi*; Yamada, Hiroshi*; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 82(2), p.109 - 110, 2006/02
no abstracts in English
Sakamoto, Yoshiteru; Toi, Kazuo*; Fukuda, Takeshi*; Fukuyama, Atsushi*; Fujita, Takaaki; Ogawa, Yuichi*; Takizuka, Tomonori; Takenaga, Hidenobu; Yagi, Masatoshi*; Yamada, Hiroshi*; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 81(8), p.626 - 627, 2005/08
no abstracts in English
Kawano, Yasunori; Kawahata, Kazuo*; Kusama, Yoshinori; Sasao, Mamiko*; Sugie, Tatsuo; Mase, Atsushi*; Asakura, Nobuyuki; Kato, Takako*; Takamura, Shuichi*; Tanabe, Tetsuo*; et al.
Purazuma, Kaku Yugo Gakkai-Shi, 81(2), p.128 - 130, 2005/02
no abstracts in English
by multiphoton excitation in alcohol solutionsMatsuda, Shohei; Nakashima, Nobuaki*; Yokoyama, Keiichi; Yatsuhashi, Tomoyuki*; Chosrowjan, H.*; Taniguchi, Seiji*; Somekawa, Toshihiro*
no journal, ,
The photoredox reactions of lanthanides as a photochemical separative technique proposed in the 1970s are expected to be applied for recovery and recycling of rare metals and nuclear fuel reprocessing. In this study, Eu ions in methanol and ethanol solutions were reduced to Eu
ions upon irradiation with nanosecond laser pulses. Eu was observed when the laser wavelength was tuned to f-f transition bands at 394 and 465 nm. The formation rate of Eu was proportional to the square of the incident laser intensity, indicating that the photoreduction is induced by a two-photon process. The efficiency of the photoreduction in ethanol was higher than that in methanol.
Matsuda, Shohei; Nakashima, Nobuaki*; Yokoyama, Keiichi; Yatsuhashi, Tomoyuki*; Chosrowjan, H.*; Taniguchi, Seiji*; Somekawa, Toshihiro*
no journal, ,
The photochemical reactions of lanthanide and actinide ions are expected to be utilized for recovery and recycling of rare earths, and nuclear fuel reprocessing. Heading towards research for implementation of photochemical purification, a unified evaluation of the efficiency of multiphoton processes is required. In this study, we have evaluated the fluence dependence of photoreduction of Eu(III) more precisely than before by measuring the beam profile of the excitation laser. The reduction rate of Eu(III)
Eu(II) is proportional to the square of the laser fluence, indicating that the photoreduction of Eu(III) is induced by a two-photon process.
